A Cherry Protein and Its Gene, Abundantly Expressed in Ripening Fruit, Have Been Identified as Thaumatin-Like

International audienc

Expression patterns of cell wall-modifying genes from banana during fruit ripening and in relationship with finger drop

Few molecular studies have been devoted to the finger drop process that occurs during banana fruit ripening. Recent studies revealed the involvement of changes in the properties of cell wall polysaccharides in the pedicel rupture area. In this study, the expression of cell-wall modifying genes was monitored in peel tissue during post-harvest ripening of Cavendish banana fruit, at median area (control zone) and compared with that in the pedicel rupture area (drop zone). To this end, three pectin methylesterase (PME) and seven xyloglucan endotransglycosylase/hydrolase (XTH) genes were isolated. The accumulation of their mRNAs and those of polygalaturonase, expansin, and pectate lyase genes already isolated from banana were examined. During post-harvest ripening, transcripts of all genes were detected in both zones, but accumulated differentially. MaPME1, MaPG1, and MaXTH4 mRNA levels did not change in either zone. Levels of MaPME3 and MaPG3 mRNAs increased greatly only in the control zone and at the late ripening stages. For other genes, the main molecular changes occurred 1–4 d after ripening induction. MaPME2, MaPEL1, MaPEL2, MaPG4, MaXTH6, MaXTH8, MaXTH9, MaEXP1, MaEXP4, and MaEXP5 accumulated highly in the drop zone, contrary to MaXTH3 and MaXTH5, and MaEXP2 throughout ripening. For MaPG2, MaXET1, and MaXET2 genes, high accumulation in the drop zone was transient. The transcriptional data obtained from all genes examined suggested that finger drop and peel softening involved similar mechanisms. These findings also led to the proposal of a sequence of molecular events leading to finger drop and to suggest some candidates

Maturation of sweet cherry is accompanied by an accumulation to a high level of a thaumatin-like protein (PR protein type V).

Texte du poster présenté à la Gordon Conference * INRA, URD, Domaine St Paul, Site Agroparc, 84914 Avignon cedex 9 Diffusion du document : INRA, URD, Domaine St Paul, Site Agroparc, 84914 Avignon cedex 9International audienc

Changes in glycosidase activities during development and ripening of melon

International audienc

Recolte mecanique de la cerise douce: le point sur les phenomenes d'abscission naturels ou provoques, et sur la prevision de la date de maturite

National audienc

Tomato peroxidasic system

National audienc

Tomato peroxidasic system

National audienc

Ripening and overripening of a non climacteric fruit : the sweet cherry Bigarreau Napoleon. 1. Requirements for extraction of polyribosomes from lyophilized tissue

International audienc

Sequence of AFTP1, a cysteine proteinase from apricot fruit (Accession nø U93166). Gene expression during fruit ripening

url=http://www.tarweed.com/pgr/PGR97-179.htmlInternational audienc

Molecular cloning and nucleotide sequence of a metallothionein-like protein from apricot fruit (Accession nø U97494). Gene expression during fruit ripening

url=http://www.tarweed.com/pgr/PGR97-160.htmlInternational audienc