Evolution of Quorum Sensing in <i>Pseudomonas aeruginosa</i> Can Occur via Loss of Function and Regulon Modulation

Pseudomonas aeruginosa populations evolving in cystic fibrosis lungs, animal hosts, natural environments and in vitro undergo extensive genetic adaption and diversification. A common mutational target is the quorum sensing (QS) system, a three-unit regulatory system that controls the expression of virulence factors and secreted public goods. Three evolutionary scenarios have been advocated to explain selection for QS mutants: (i) disuse of the regulon, (ii) cheating through the exploitation of public goods, or (ii) modulation of the QS regulon. Here, we examine these scenarios by studying a set of 61 QS mutants from an experimental evolution study. We observed nonsynonymous mutations in all three QS systems: Las, Rhl, and Pseudomonas Quinolone Signal (PQS). The majority of the Las mutants had large deletions of the Las regulon, resulting in loss of QS function and the inability to produce QS-regulated traits, thus supporting the first or second scenarios. Conversely, phenotypic and gene expression analyses of Rhl mutants support network modulation (third scenario), as these mutants overexpressed the Las and Rhl receptors and showed an altered QS-regulated trait production profile. PQS mutants also showed patterns of regulon modulation leading to strain diversification and phenotypic tradeoffs, where the upregulation of certain QS traits is associated with the downregulation of others. Overall, our results indicate that mutations in the different QS systems lead to diverging effects on the QS trait profile in P. aeruginosa populations. These mutations might not only affect the plasticity and diversity of evolved populations but could also impact bacterial fitness and virulence in infections

Evolution of the quorum sensing systems in Pseudomonas aeruginosa can involve both loss of regulon function and network modulation

Pseudomonas aeruginosa populations evolving in cystic fibrosis (CF) lungs, animal infection models, natural environments or in vitro undergo extensive genetic adaption and diversification. A common mutational target is the quorum sensing (QS) regulon, a three-unit regulatory system that controls the expression of a suite of virulence factors and secreted public goods. Three scenarios have been advocated to explain selection for QS mutants, which include (I) disuse of the regulon, (II) cheating on public goods, or (III) modulation of the network. Here, we test these scenarios by examining a set of 61 QS mutants from an experimental evolution study. We observed non-synonymous mutations in all three QS systems – Las, Rhl and PQS. Most Las mutants carried large deletions, resulting in loss of QS function, and the inability to produce QS-regulated traits (scenario I or II). Conversely, phenotypic and gene expression analyses of Rhl mutants support network modulation (scenario III), as these mutants overexpressed the Las and Rhl regulators and showed an altered QS-regulated trait production portfolio. PQS mutants also showed patterns of network modulation (scenario III), spurring strain diversification and phenotypic trade-offs, where the upregulation of certain QS traits is associated with the downregulation of others. Overall, our results indicate that mutations in different QS systems lead to diverging effects on the social portfolio of bacterial populations. These mutations might not only affect the plasticity and diversity of evolved populations but could also impact bacterial fitness and virulence in infections. Importance - Pseudomonas aeruginosa uses quorum sensing (QS), a three-unit multi-layered network, to coordinate expression of traits for growth and virulence in the context of infections. Despite its importance for bacterial fitness, the QS regulon appears to be a common mutational target during long-term adaptation of P. aeruginosa in the host, natural environments and experimental evolutions. This raises the questions why such an important regulatory system is under selection and how mutations change the portfolio of QS-regulated traits. Here, we examine a set of 61 naturally evolved mutants to address these questions. We found that mutations involving the master regulator, LasR, resulted in an almost complete breakdown of QS, while mutations in RhlR and PqsR resulted in modulations of the QS regulon, where both the QS regulon structure and the QS-regulated trait portfolio changed. Our work reveals that natural selection drives diversification in QS activity patterns in evolving populations

Desenvolvimento da área foliar e matéria seca da folha de feijão-caupi em relação aos graus-dia e radiação.

O acúmulo térmico (graus-dia) e a radiação têm sido utilizados em modelos de simulação de crescimento, desenvolvimento e produção em várias culturas. Objetivando verificar a relação entre a área foliar e peso seco de folhas com graus-dia e radiação, desenvolveu-se um estudo. A mensuração da área foliar e matéria seca das folhas, no total de 50 por coleta, foram realizadas de 7 em 7 dias. A área foliar foi medida utilizando um digitalizador de imagens. Após a digitalização das imagens, utilizou-se o programa Image Tool v. 2.0, no qual foi capaz determinar a área foliar. Após a leitura da área foliar as folhas foram submetidos a secagem em estufa de circulação de ar a 600C até peso constante para se obter a fitomassa. Os resultados mostraram que o aumento da área foliar não teve uma relação com o graus-dia e radiação, entretanto a quantidade de matéria seca de folha apresentou uma forte relação com estes dois fatores com a mesma magnitude. Portanto a possibilidade de se obter modelos para simulação da matéria seca de plantas de feijão-caupi poderá vir a ser uma alternativa em futuros estudos

Heat conduction in the hollow sphere with a power-law variation of the external heat transfer coefficient

The conduction phenomenon in an insulated sphere is re-worked through a dimensionless approach, where the heat transfer coefficient dependence on the external radius and on the surface temperature, as in the case of forced and free convection, is taken into account. Assuming a power law variation of the convection coefficient [1, 2], and using the results of Sparrow [3], equations and graphs for the most important dimensionless parameters are presented. The developed equations show, for example, that as the insulation thickness increases the heat transfer rate tends to a positive value, independent of the considered case: constant convection coefficient, forced or free convection.http://www.sciencedirect.com/science/article/B6V3J-42MP1M2-3/1/6228c6d77bbaac53e7833e6cd67d8ef

On extra forces from large extra dimensions

The motion of a classical test particle moving on a 4-dimensional brane embedded in an $n$-dimensional bulk is studied in which the brane is allowed to fluctuate along the extra dimensions. It is shown that these fluctuations produce three different forces acting on the particle, all stemming from the effects of extra dimensions. Interpretations are then offered to describe the origin of these forces and a relationship between the 4 and $n$-dimensional mass of the particle is obtained by introducing charges associated with large extra dimensions.Comment: 9 pages, no figuer