Melhoramento genético de microrganismos para produção de biocombustíveis e químicos renováveis: integração de abordagens de biologia molecular, biologia sistêmica, biologia sintética e engenharia metabólica.

bitstream/item/80574/1/Agroenergia-em-revista-n.5-Leia.pd

Enzymes: Bioprospecting of fungi that produce enzyme complexes.

Elite strains of endophytic fungi, isolated from the Brazilian biodiversity, producers of efficient enzyme complexes for hydrolysis of the pre-treated sugarcane bagasse.TC 162

Production of cellulolytic and hemicellulolytic enzymes by Aspergillus niger 3T5B8 mutants and parental strains in solid-state fermentation.

Several studies have sought to increase the efficiency of the enzymatic hydrolysis of lignocellulosic biomass. In this context, the improvement of strains producing cellulolytic and hemicellulolytic enzymes may result in more efficient enzymatic mixtures. The parent strain of Aspergillus niger 3T5B8, referred as a polygalacturonase producer, was used for genetic improvement in order to increase the production of cellulases and hemicellulases. The production of the enzymes CMCase, xylanase, beta-glucosidase and polygalacturonase by solid-state fermentation using two mutant strains P49 and P83 was evaluated and compared with the parental strain. The results showed a prominence for the P83 strain with an increase in the production of 174% for xylanase and 402% for polygalacturonase.SINAFERM; SHEB. 3 a 6 de setembro. Seção Trabalhos. Ref. 56374