FAVARO, L. C. de L.

MARTINS, P. A.

PACHECO, T. F.

SALUM, T. F. C.

SCIUTO, D. L.

English

Repository Open Access to Scientific Information from Embrapa

05/12/2017 39th Symposium on Biotechnology for Fuels and Chemicalshttps://sim.confex.com/sim/39th/meetingapp.cgi/Paper/33736 1/1M40: A new lipase for biodiesel synthesisPAPERMonday, May 01, 20176:00 PM - 8:00 PMSan Francisco Marriott Marquis - Yerba Buena Salons 9, B2 Lower LevelBiodiesel is a fuel produced industrially through alkaline chemical transesterification. Lipases can be used to produce biodiesel with theadvantage of being more environmentally friendly. However, the enzymatic process is still expensive compared to the chemical process. Inthis context, new lipases discovery and the utilization of low-cost cultivation media are necessary to reach the economic viability of enzymaticbiodiesel production. Hence, this study aimed to prospect new microorganism strains and to produce lipases by solid-state fermentation inwheat bran. Then, 298 microorganisms (bacteria, yeasts and filamentous fungi) were isolated from oil palm fruits. They were evaluated byqualitative tests in solid media containing triolein and olive oil as a carbon source and quantitative tests in solid-state fermentation usingwheat bran. We selected the strain BDA-FI 7, an Aspergillus sp., to optimize lipase production. A Central Composite Rotatable Design (CCRD)was performed. The variables evaluated were: temperature (20-35 °C), moisture (45-65 %) and inoculum concentration (10 -10  spores/g).Flasks were incubated, and after 168 h, lipase was extracted from the solid substrate and its pNPP-hydrolyzing activity was determined. Thebest lipase activity obtained was 55.9 U/g dry substrate. The conditions that maximize the lipase activity are 35 °C, 65 % moisture and aninoculum of 5.05 x 10  spores/g. At this combination, the maximum predicted yield was 58.4 U/g. Preliminary studies showed that this lipasecan catalyze biodiesel synthesis (ethyl esters) in n-heptane as a solvent.6 87AuthorsPedro Alves MartinsDébora Lo SciutoLéia Cecília de Lima FavaroThályta Fraga PachecoThaís Fabiana Chan SalumEmbrapa AgroenergyEmbrapa AgroenergyEmbrapa AgroenergyEmbrapa AgroenergyEmbrapa AgroenergyView Related EventsDay: Monday, May 01, 2017

M40: A new lipase for biodiesel synthesis.

http://www.alice.cnptia.embrapa.br/bitstream/doc/1081676/1/ComprovacaoAINFO39thSymposiumonBiotechnologyforFuelsandChemicals.pdf

M40: A new lipase for biodiesel synthesis.

Abstract

Similar works

Full text

Available Versions

Repository Open Access to Scientific Information from Embrapa