466 research outputs found
Аргументация как лингвистический элемент в полемике. Виды аргументов
Thermoanaerobacter brockiifermented serine to acetate and ethanol. It oxidized leucine to isovalerate, isoleucine to 2-methylbutyrate, and valine to isobutyrate only in the presence of thiosulfate, or when co-cultured withMethanobacteriumsp. This oxidative deamination was rendered thermodynamically possible by the ability ofT. brockiito reduce thiosulfate to sulfide or the transfer of reducing equivalents to the hydrogenotrophic methanogen. The results suggest thatT. brockiimay be of ecological significance in thermal environments in the turnover of amino acids, especially with thiosulfate or H2-utilizing methanogens are present
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Several members of the order Thermotogales in the domain Bacteria, viz., Thermotoga neapolitana, Thermotoga maritima, Thermosipho africanus, Fervidobacterium islandicum, and Thermotoga strain SEBR 2665, an isolate from an oil well, reduced thiosulfate to sulfide. This reductive process enhanced cellular yields and growth rates of all the members but was more significant with the two hyperthermophiles T. neapolitana and T. maritima. This is the first report of such an occurrence in this group of thermophilic and hyperthermophilic anaerobic bacteria. The results suggest that thiosulfate reduction is important in the geochemical cycling of sulfur in anaerobic thermal environments such as the slightly acidic and neutral-pH volcanic hot springs and oil reservoirs
Effect of thiosulphate as electron acceptor on glucose and xylose oxidation by Thermoanaerobacter finnii and Thermoanaerobacter sp. isolated from oil field water
#Thermoanaerobacter finnii fermente le glucose et le xylose en lactate, acétate, H2, CO2 et éthanol qui est le produit majeur du métabolisme. Une autre bactérie du genre #Thermoanaerobacter isolée d'une eau de gisement pétrolier produit des quantités aussi importantes d'éthanol que de lactate à partir de ces sucres. L'oxydation du glucose et du xylose par ces deux micro-organismes est complètement modifiée en présence de thiosulfate puisque, dans ces conditions, l'acétate devient le produit majeur du métabolisme des sucres. Cette modification du métabolisme amène à de meilleurs rendements cellulaires et à un meilleur taux de croissance bactérienne lorsque le thiosulfate est utilisé comme accepteur d'électrons. Une plus grande production d'acétate en présence de thiosulfate indique que les bactéries testées récupèrent plus d'énergie des phosphorylations liées au substrat. Ces modifications observées sur le métabolisme des sucres en présence de thiosulfate nous amènent à tenir compte plus précisément du rôle que peut jouer cet accepteur d'électrons dans l'oxydation de la matière organique dans les sources chaudes riches en sulfate sulfuré ou les eaux de gisements pétroliers. (Résumé d'auteur
Peptide and amino acid oxidation in the presence of thiosulfate by members of the genus Thermoanaerobacter
#Thermoanaerobacter brockii, #T. ethanolicus, #T. thermohydrosulfuricus, #T. finnii, and #Thermoanaerobacter strain SEBR 5268 (an isolate from an oil-producting well) were studied for their ability to oxidize proteinaceous compounds that included gelatin, peptides, and casamino acids. All bacteria tested used peptides and amino acids, but only slightly. However, in the presence of thiosulfate all the #Thermoanaerobacter species showed a substantial improvement in growth and/or the production of acetate, isovalerate, isobutyrate, and sulfide. Propionate was a minor product of peptide or amino acid oxidation. The reduction of thiosulfate during growth on peptides by members of the #Thermoanaerobacter$ species is a trait that closely resembles that of archaeal hyperthermophiles during growth on peptides and amino acids with elemental sulfur as electron acceptor. (Résumé d'auteur
Monte Carlo simulation of migration of fusion plasma neutrons - optimization and traps
We recall some of the sensitive points and stages in random walk of neutron, frequent solutions and their consequences on quality and duration of MC neutronic and photonic simulations. We present some unconventional approaches we developed to precisely meet the double paramount MC goal: maximal probing at minimal variance, whence minimum CPU time. Instead of the traditional point observation, enhanced probing is used to limit collected random scatter dispersion. Vector probing by shower (through ”nuclear reaction‑channel” space) and drizzle (improved sampling throughout space involving even the deepest parts, with region fragmentation allowed) drastically reduces the collected variance. By treating analogically close‑collision flux-at-a-point tallies, the unphysical pole discontinuity at-the-detecting-point is avoided - this allows the study of even within-detector collisions. For deep shielding treatment the use of the two-step Cascade Monte Carlo is recommended as it reproduces from physical considerations the mathematical approach. Making sure to distinguish volumetric versus local destructive effects, the latter requiring the use of "statistics of extreme values"
Isolation and characterization of a new CO-utilizing strain, Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans, isolated from a geothermal spring in Turkey
A novel anaerobic, thermophilic, Gram-positive, spore-forming, and sugar-fermenting bacterium (strain TLO) was isolated from a geothermal spring in Ayaş, Turkey. The cells were straight to curved rods, 0.4–0.6 μm in diameter and 3.5–10 μm in length. Spores were terminal and round. The temperature range for growth was 40–80°C, with an optimum at 70°C. The pH optimum was between 6.3 and 6.8. Strain TLO has the capability to ferment a wide variety of mono-, di-, and polysaccharides and proteinaceous substrates, producing mainly lactate, next to acetate, ethanol, alanine, H2, and CO2. Remarkably, the bacterium was able to grow in an atmosphere of up to 25% of CO as sole electron donor. CO oxidation was coupled to H2 and CO2 formation. The G + C content of the genomic DNA was 35.1 mol%. Based on 16S rRNA gene sequence analysis and the DNA–DNA hybridization data, this bacterium is most closely related to Thermoanaerobacter thermohydrosulfuricus and Thermoanaerobacter siderophilus (99% similarity for both). However, strain TLO differs from Thermoanaerobacter thermohydrosulfuricus in important aspects, such as CO-utilization and lipid composition. These differences led us to propose that strain TLO represents a subspecies of Thermoanaerobacter thermohydrosulfuricus, and we therefore name it Thermoanaerobacter thermohydrosulfuricus subsp. carboxydovorans
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