Limpeza clonal.

bitstream/item/140367/1/limpeza-clonal.pd

Limpeza clonal: cuidados com as mudas para obter um vinhedo sadio.

bitstream/item/140369/1/limpeza-clonal-pac.pd

Obtenção de material propagativo livre de vírus e diagnóstico de vírus em macieiras e pereiras.

Principais vírus da macieira e da pereira. Danos causados por vírus. Limpeza clonal: produção de macieiras e pereiras livres de vírus. Análises e testes de avaliação de sanidade.bitstream/item/60758/1/CNPUV-DOC.-69-09.pd

Detection of Viruses in Apples and Pears by Real Time RT-PCR Using 5'-Hydrolysis Probes

Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV) and Apple mosaic virus (ApMV) are common in apples and pears and the main targets of virus elimination from propagation material. The objective of this work was to design primers and probes for a real time RT-PCR protocol for detection of the four above viruses. FAM/TAMRA-labeled probes and primers were designed by searching for highly conserved nucleotide regions in the coat protein gene of the four viruses. Infection levels in analyzed apple samples were 92.6, 96.4, 100 and 88% for ASGV, ASPV, ACLSV and ApMV, respectively. In pears, all pre-existing ASPV infections were detected. Viral infections were confirmed in a selection of commercial cultivars of apples and pear scions, and quince rootstocks, demonstrating the sensitivity and reliability of the designed primers and probes. Real time RT-PCR using 5'-labeled probes is suitable for checking sanitary quality as a routine test in certification programs

Importância da origem do material de propagação na qualidade da muda de videira.

bitstream/CNPUV/8128/1/cir050.pdfDisponível também no formato online

Doenças de uva.

São conhecidas na videira (Vitis spp.) dezenas de doenças consideradas de origem viral. A videira, por ser propagada vegetativamente, facilita a disseminação destes patógenos e favorece o aparecimento de doenças complexas, pelo acúmulo de diferentes vírus numa mesma planta

First report on detection of three Bunya-Like Viruses in apples in Brazil.

Apple chlorotic leaf spot virus (ACLSV), apple stem grooving virus (ASGV), and apple stem pitting virus (ASPV) cause significant losses to Brazilian (BR) apple production. Looking beyond these latent viruses, high-throughput sequencing (HTS) of three samples (Vacaria, Brazil) was performed on an Illumina HiSeq X Ten platform (USA), cv. Braeburn (BB), and a BGISEQ-500 platform (China), cvs. Royal Gala (RG) and Mishima (MI)

Detecção e caracterização molecular dos genes da proteína capsidial de ilarvírus e ampelovírus que infectam fruteiras temperadas.

Dentre os principais patógenos que incidem em fruteiras temperadas, destacam-se o Prune dwarf virus (PDV), o Apple mosaic virus (ApMV) e o Grapevine leafroll-associated virus 1 (GLRaV-1). Neste trabalho foram realizadas a detecção e a caracterização molecular dos genes da proteína capsidial de isolados destas três espécies virais. RNAs totais foram extraídos de amostras de folhas de pessegueiros, macieiras e videiras e, nas reações de RT-PCR, foram utilizados oligonucleotídeos específicos para cada espécie viral. Os cDNAs amplificados foram clonados e sequenciados. Foram verificadas altas identidades entre as sequências de nucleotídeos dos genes da proteína capsidial dos isolados brasileiros de PDV, ApMV e GLRaV-1 e isolados de outros países, independente da origem geográfica e da hospedeira. O peso molecular da proteína capsidial destes vírus foi estimado por meio de Western blot em cerca de 24kDa (PDV), 26kDa (ApMV) e 39kDa (GLRaV-1).Nota técnica

First report of grapevine pinot gris virus infecting grapevine in Brazil.

Grapevine Pinot gris virus (GPGV) has been re-ported infecting grapevines (Vitisspp.) in several countriesof the world. In this study, 19.5% of grapevine samples col-lected in Brazil and indexed for GPGV by RT-qPCR wasinfected. The complete coat protein and the partial replicase genes of two Brazilian isolates (CF-BR and ME-BR) were sequenced and exhibited very high nucleotide and deduced amino acid identities with many foreign characterized isolates of GPGVavailable in the GenBank. This is the first detection of GPGV infecting grapevines in Brazil. Keywords GPGV . Trichovirus . RT-qPCR . Real time RT-PCR. Diagnosis.Disponível em: . Acesso em: 04 set. 2017