Optimization of cultivation conditions for extracellular lipase production by Yarrowia lipolytica using response surface method

A wild strain of Yarrowia lipolytica, identified as LMI 91, was isolated from an oleaginous Brazilian fruit called pequi (Caryocar brasiliense Camb), which is a native species of the Brazilian savannah and which acts as a potent lipase producer. The present study examined the effects of certain factors, such as the combination of casein and meat peptones, as well as the initial pH of a culture medium in lipase produced by Y. lipolytica LMI 91. Experiments were conducted within a low concentration of olive oil, at a temperature of 30°C and a fermentation time of 60 h, under orbital shaking. The response surface methodology was used to determine the optimum concentration of the constituents’ composition. A 22 experimental design with four axial points and three central points was employed in the optimization experiments, and interactive regression analysis was performed to obtain the optimum concentration. The experimental values were found to be in accordance with the predicted values, presenting a correlation coefficient of 0.866. The maximum lipase activity (13.0 U/mL) was obtained by peptone mixture of 70 g/L (1:1) and an initial pH of 5.0.Keywords: Yarrowia lipolytica, wild strain, extracellular lipase, fermentation, optimizationAfrican Journal of Biotechnology Vol. 12(17), pp. 2270-227

Fag Rag June 1971

Issue 1 of Fag Rag, a gay liberation newspaper published in Boston, MA in June 1971.https://digitalcommons.usm.maine.edu/fagrag/1000/thumbnail.jp

Fag Rag Fall-Winter 1973

Issue 6 of Fag Rag, a gay liberation newspaper published in Boston, MA in fall-winter of 1973.https://digitalcommons.usm.maine.edu/fagrag/1005/thumbnail.jp

Fag Rag Fall 1974

Issue 10 of Fag Rag, a gay liberation newspaper published in Boston, MA in the fall of 1974.https://digitalcommons.usm.maine.edu/fagrag/1008/thumbnail.jp

Fag Rag Summer 1975

Issue 13 of Fag Rag, a gay liberation newspaper published in Boston, MA in the summer of 1975.https://digitalcommons.usm.maine.edu/fagrag/1011/thumbnail.jp

Fag Rag Fall 1971

Issue 2 of Fag Rag, a gay liberation newspaper published in Boston, MA in the fall of 1971.https://digitalcommons.usm.maine.edu/fagrag/1001/thumbnail.jp

Fag Rag February-March 1976

Issue 15 of Fag Rag, a gay liberation newspaper published in Boston, MA in the February-March of 1976.https://digitalcommons.usm.maine.edu/fagrag/1013/thumbnail.jp

Fag Rag Fall 1978

Double issue 23 and 24 of Fag Rag, a gay liberation newspaper published in Boston, MA in the fall of 1978.https://digitalcommons.usm.maine.edu/fagrag/1016/thumbnail.jp

Fag Rag Winter 1974

Issue 11 of Fag Rag, a gay liberation newspaper published in Boston, MA in the winter of 1974.https://digitalcommons.usm.maine.edu/fagrag/1009/thumbnail.jp