Sweet-and-salty biocatalysis : fructooligosaccharides production using Cladosporium cladosporioides in seawater

Production of fructooligosaccharides (FOS) from sucrose was obtained using a bioprocess entirely performed in seawater. The halophilic fungus Cladosporium cladosporioides MUT 5506 was grown in a seawater-based medium and mycelium displayed an optimal activity in seawater at 50\u201360 \ub0C, being stable up to 60 \ub0C. Under optimized conditions in seawater (50 \ub0C, sucrose 600 g/L, lyophilized mycelium 40 g/L), C. cladosporioides gave a maximum FOS yield of 344 g/L after 72 h with a preponderance of 1F-FOS derivatives (1-kestose 184 g/L, 1-nystose 98 g/L and 1-fructofuranosylnystose 22 g/L), and the noteworthy presence of the non-conventional disaccharide blastose (30 g/L after 144 h). Lyophilized mycelium exhibited good stability in seawater (76% of the initial activity was retained after 15 cycles of reutilization). This proof-of-concept application reports for the first time the production of FOS in a non-conventional medium such as seawater

An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides

A sustainable and scalable process for the production of a new mixture of fructooligosaccharides (FOS) was developed using a continuous-flow approach based on an immobilized whole cells-packed bed reactor. The technological transfer from a classical batch system to an innovative flow environment allowed a significant improvement of the productivity. Moreover, the stability of this production system was ascertained by up to 7 days of continuous working. These results suggest the suitability of the proposed method for a large-scale production of the desired FOS mixture, in view of a foreseeable use as a novel prebiotic preparation

Stability and stabilization of biocatalysts

Centro de Informacion y Documentacion Cientifica (CINDOC). C/Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai

Solubility of glucose in mixtures containing 2-methyl-2- butanol, dimethyl sulfoxide, acids, esters, and water

Solubility measurements of glucose in a variety of binary, ternary, and multicomponent mixtures containing 2-methyl-2- butanol, dimethyl sulfoxide, acids, esters, and water at different temperatures are presented. The solubilities of crystalline beta-glucose, amorphous beta-glucose, and amorphous beta-glucose in 2-methyl-2-butanol at 60 degreesC have also been measured. The results show that the solubilities of the amorphous forms in 2-methyl-2-butanol are higher than that of the corresponding crystalline form. The presence of dimethyl sulfoxide significantly increases glucose solubility in 2- methyl-2-butanol. Finally, the presence of glucose ester increases glucose solubility in 2-methyl-2-butanol, while the presence of fatty acid has the opposite effect