Fibromyalgia and Shoulder Surgery : a Systematic Review and a Critical Appraisal of the Literature

Fibromyalgia is a common musculoskeletal syndrome characterized by chronic widespread pain and other systemic manifestations, which has demonstrated a contribution to higher postoperative analgesic consumption to other surgeries such as hysterectomies and knee and hip replacements. The aim of this review is to search current literature for studies considering the impact of fibromyalgia on clinical outcomes of patients undergoing shoulder surgery. A systematic literature review was conducted in PubMed/Medline, Embase, and ClinicalTrials.gov in February 2019. Studies were selected based on the following participants, interventions, comparisons, outcomes, and study design criteria: adult patients undergoing surgery for shoulder pain (P); diagnosis of fibromyalgia (I); patients without fibromyalgia (C); outcome of surgery in terms of pain or analgesic or non-steroidal anti-inflammatory drugs consumption (O); case series, retrospective studies, observational studies, open-label studies, randomized clinical trials, systematic reviews and meta-analyses were included (S). Authors found 678 articles, of which four were found eligible. One retrospective study showed that patients with fibromyalgia had worse clinical postoperative outcomes; two retrospective studies reported a higher opioid prescription in patients with fibromyalgia and one prospective observational study found that a higher fibromyalgia survey score correlated with lower quality of recovery scores two days after surgery. The scarce and low-quality evidence available does not allow confirming that fibromyalgia has an impact on postoperative outcomes in shoulder surgery. Future studies specifically focusing on shoulder surgery outcomes may help improvement and personalization of the management of patients with fibromyalgia syndrome (PROSPERO 2019, CRD42019121180)

Study of the wettability behavior of stainless steel surfaces after ultrafast laser texturing

The interest in superhydrophobic surfaces has grown exponentially over recent decades. Since the lotus leaf dual hierarchical structure was discovered, researchers have investigated the foundations of this behavior and many methods have been developed to obtain superhydrophobic surfaces. In this paper the possibility to use ultrafast laser treatments to obtain hydrophobic and superhydrophobic stainless surfaces was investigated on a AISI 316L stainless steel, ranging the total energy doses provided to the surfaces from 178 to 1143 J/cm2. As SEM-FEG images reveals, different surface microstructures can be obtained at the increasing values of energy dose. Independently on the specific values of laser treatment, all the obtained samples showed hydrophobic values of static contact angle. However, only particular surface microstructures allowed obtaining a self-cleaning surface characterized by low values of both contact angle hysteresis and roll-off angle. The obtained results led to define the effect of the laser parameters on the morphological, chemical and wetting surface properties allowing one to design new textures with the desired wetting properties, from “lotus effect” surfaces to “rose petal effect” surfaces

A systematic review of the biological processes involved in deep-brain stimulation for parkinson’s disease: A focus on the potential disease-modifying effects

Deep-Brain Stimulation (DBS) is an important treatment option for the management of Parkinson’s disease (PD) and is a common symptomatic treatment. However, an increasing number of studies have examined the biological processes to assess if DBS can also modify the natural history of PD by acting on its pathophysiological mechanisms. Relevant literature published up to November 2020 was systematically searched on databases such as PubMed, ISI Web of Knowledge, Academic Search Index, and Science Citation Index. The following predefined inclusion criteria were applied to the full-text versions of the selected articles: I) recruiting and monitoring of PD subjects that were previously treated with DBS and ii) investigating the electrophysiological, biochemical, epigenetic, or neuroimaging effects of DBS. Studies focusing exclusively on motor and clinical changes were excluded. Reviews, case reports, studies on animal models, and computational studies were also not considered. Out of 2,960 records screened, 43 studies met the inclusion criteria. Only three studies described a potential disease-modifying effect of DBS. However, a wide heterogeneity was observed in the investigated biomarkers, and the design and methodological issues of several studies limited their ability to find potential disease-modifying features. Specifically, 60.4% of the trials followed-up subjects for no more than 1 year from the surgical intervention, and 67.4% observed patients with PD only once after DBS. Moreover, 64.2% of the studies enrolled late-stage PD patients. Most of the studies (88.4%) reported that DBS only had a symptomatic effect, with several of them showing some limitations in the study design and recruitment of patients. Further studies using shared biomarkers are encouraged to assess if and how DBS might affect the progression of PD. Based on the existing preclinical literature, prospective clinical trials examining the course of PD in early-stage patients are needed

Use of biomarkers in ongoing research protocols on alzheimer’s disease

The present study aimed to describe and discuss the state of the art of biomarker use in ongoing Alzheimer’s disease (AD) research. A review of 222 ongoing phase 1, 2, 3, and 4 protocols registered in the clinicaltrials.gov database was performed. All the trials (i) enrolling subjects with clinical disturbances and/or preclinical diagnoses falling within the AD continuum; and (ii) testing the efficacy and/or safety/tolerability of a therapeutic intervention, were analyzed. The use of biomarkers of amyloid deposition, tau pathology, and neurodegeneration among the eligibility criteria and/or study outcomes was assessed. Overall, 58.2% of ongoing interventional studies on AD adopt candidate biomarkers. They are mostly adopted by studies at the preliminary stages of the drug development process to explore the safety profile of novel therapies, and to provide evidence of target engagement and disease-modifying properties. The biologically supported selection of participants is mostly based on biomarkers of amyloid deposition, whereas the use of biomarkers as study outcomes mostly relies on markers of neurodegeneration. Biomarkers play an important role in the design and conduction of research protocols targeting AD. Nevertheless, their clinical validity, utility, and cost-effectiveness in the “real world” remain to be clarified

Effect of powder recycling in laser-based powder bed fusion of Ti-6Al-4V

Additive manufacturing (AM) has shown promise to process parts for end-use applications, however stringent requirements must be fulfilled in terms of reliability and predictability. The expensiveness of raw materials for AM, especially for metal-based Powder Bed Fusion (PBF), brings about the need for a careful recycling of powder, but the effect of powder reuse on both processing conditions and final part performance is still the focus of intensive research in the open literature. Although ASTM F2924-14 specifies the virgin-to-used powder ratio to be introduced to manufacture titanium-6aluminum-4vanadium (Ti-6Al-4V) components by PBF, a deeper understanding of the effect of powder recycling on the mechanical properties of finished parts is expected to foster a more efficient and safe reuse. The present contribution is therefore addressed to investigate the consequence of Ti- 6Al-4V powder recycling on the flowability, particle size distribution and morphology of the feedstock material as well as on the density and tensile performance of built parts. In order to quantify the recyclability of powders, a new "average usage time" (AUT) parameter is defined to account for both the real usage time of the powder and the virgin-to-used powder mixing ratio. The new parameter, whose applicability can be readily extended to any kind of feedstock powder, offers a significant contribution to achieve a more consistent and economical recycling of raw materials for PBF processing

Suppression of Parasitic Nonlinear Processes in Spontaneous Four-Wave Mixing with Linearly Uncoupled Resonators

We report on a signal-to-noise ratio characterizing the generation of identical photon pairs of more than 4 orders of magnitude in a ring resonator system. Parasitic noise, associated with single-pump spontaneous four-wave mixing, is essentially eliminated by employing a novel system design involving two resonators that are linearly uncoupled but nonlinearly coupled. This opens the way to a new class of integrated devices exploiting the unique properties of identical photon pairs in the same optical mode

Effect of a Collagen-Based Compound on Morpho-Functional Properties of Cultured Human Tenocytes

Background: Greater Trochanter Pain Syndrome (GTPS) is the main reason for recalcitrant lateral hip pain. Gluteus medius and minimus tendinopathy plays a key role in this setting. An injectable medical compound containing collagen type I (MD-Tissue, Guna) has been produced with the aim to counteract the physiological and pathological degeneration of tendons. In this study we aimed at characterizing the effect of this medical compound on cultured human gluteal tenocytes, focusing on the collagen turnover pathways, in order to understand how this medical compound could influence tendon biology and healing. Methods: Tenocytes were obtained from gluteal tendon fragments collected in eight patients without any gluteal tendon pathology undergoing total hip replacement through an anterior approach. Cell proliferation and migration were investigated by growth curves and wound healing assay, respectively. The expression of genes and proteins involved in collagen turnover were analysed by real-time PCR, Slot blot and SDS-zymography. Results: Our data show that tenocytes cultured on MD-Tissue, compared to controls, have increased proliferation rate and migration potential. MD-Tissue induced collagen type I (COL-I) secretion and mRNA levels of tissue inhibitor of matrix metalloproteinases (MMP)-1 (TIMP-1). Meanwhile, lysyl hydroxylase 2b and matrix metalloproteinases (MMP)-1 and -2, involved, respectively, in collagen maturation and degradation, were not affected. Conclusions: Considered as a whole, our results suggest that MD-Tissue could induce in tenocytes an anabolic phenotype by stimulating tenocyte proliferation and migration and COL-I synthesis, maturation, and secretion, thus favouring tendon repair. In particular, based on its effect on gluteal tenocytes, MD-Tissue could be effective in the discouraging treatment of GTPS. From now a rigorous clinical investigation is desirable to understand the real clinical potentials of this compound

Nothing is as it seems: genetic analyses on stranded fin whales unveil the presence of a fin-blue whale hybrid in the Mediterranean Sea (Balaenopteridae)

The fin whale Balaenoptera physalus is a large rorqual species occurring worldwide, mainly in temperate and subpolar zones. In contrast to many baleen whales, not all the fin whale populations show the same model of migration. In fact, migratory behaviours of this latter species range from long seasonal migration between high and low latitudes to a complete nonmigratory behaviour. A resident fin whale population was described in the Mediterranean Sea, which is also frequented by North Atlantic individuals entering through the Strait of Gibraltar in winter to feed. Between 2020 and 2021 three individuals initially identified as fin whales died along the Tyrrhenian coasts (Mediterranean Sea, Italy). Their mitochondrial DNA control region (mtDNA CR) was analysed and compared to fin whale haplotypes previously described in North Atlantic Ocean and Mediterranean Sea to identify their geographical origin. Our results show that two individuals most likely belong to the Mediterranean fin whale population, while an individual was recognised as a putative fin-blue whale hybrid (Balaenoptera physalus x Balaenoptera musculus) with a North Atlantic origin. The discovery of the first fin-blue whale hybrid in the Mediterranean Sea was confirmed by the analysis of a biparentally inherited marker, the α-lactalbumin (α-lac) nuclear gene, demonstrating that the morphological analysis alone does not allow to correctly identify hybrids, especially if intermediate characters of both parental species are not clearly distinguishable

The ATP-binding cassette transporter A1 regulates phosphoantigen release and Vγ39Vδ2 T cell activation by dendritic cells

V\uce\ub339V\uce\ub42 T cells are activated by phosphoantigens, such as isopentenyl pyrophosphate (IPP), which is generated in the mevalonate pathway of antigen-presenting cells. IPP is released in the extracellular microenvironment via unknown mechanisms. Here we show that the ATP-binding cassette transporter A1 (ABCA1) mediates extracellular IPP release from dendritic cells (DC) in cooperation with apolipoprotein A-I (apoA-I) and butyrophilin-3A1. IPP concentrations in the supernatants are sufficient to induce V\uce\ub339V\uce\ub42 T cell proliferation after DC mevalonate pathway inhibition with zoledronic acid (ZA). ZA treatment increases ABCA1 and apoA-I expression via IPP-dependent LXR\uce\ub1 nuclear translocation and PI3K/Akt/mTOR pathway inhibition. These results close the mechanistic gap in our understanding of extracellular IPP release from DC and provide a framework to fine-tune V\uce\ub339V\uce\ub42 T cell activation via mevalonate and PI3K/Akt/mTOR pathway modulation