176 research outputs found
Towards single-electron metrology
We review the status of the understanding of single-electron transport (SET)
devices with respect to their applicability in metrology. Their envisioned role
as the basis of a high-precision electrical standard is outlined and is
discussed in the context of other standards. The operation principles of single
electron transistors, turnstiles and pumps are explained and the fundamental
limits of these devices are discussed in detail. We describe the various
physical mechanisms that influence the device uncertainty and review the
analytical and numerical methods needed to calculate the intrinsic uncertainty
and to optimise the fabrication and operation parameters. Recent experimental
results are evaluated and compared with theoretical predictions. Although there
are discrepancies between theory and experiments, the intrinsic uncertainty is
already small enough to start preparing for the first SET-based metrological
applications.Comment: 39 pages, 14 figures. Review paper to be published in International
Journal of Modern Physics
Substrate Adhesion Regulates Sealing Zone Architecture and Dynamics in Cultured Osteoclasts
The bone-degrading activity of osteoclasts depends on the formation of a cytoskeletal-adhesive super-structure known as the sealing zone (SZ). The SZ is a dynamic structure, consisting of a condensed array of podosomes, the elementary adhesion-mediating structures of osteoclasts, interconnected by F-actin filaments. The molecular composition and structure of the SZ were extensively investigated, yet despite its major importance for bone formation and remodelling, the mechanisms underlying its assembly and dynamics are still poorly understood. Here we determine the relations between matrix adhesiveness and the formation, stability and expansion of the SZ. By growing differentiated osteoclasts on micro-patterned glass substrates, where adhesive areas are separated by non-adhesive PLL-g-PEG barriers, we show that SZ growth and fusion strictly depend on the continuity of substrate adhesiveness, at the micrometer scale. We present a possible model for the role of mechanical forces in SZ formation and reorganization, inspired by the current data
Somatic mosaicism and common genetic variation contribute to the risk of very-early-onset inflammatory bowel disease
Abstract: Very-early-onset inflammatory bowel disease (VEO-IBD) is a heterogeneous phenotype associated with a spectrum of rare Mendelian disorders. Here, we perform whole-exome-sequencing and genome-wide genotyping in 145 patients (median age-at-diagnosis of 3.5 years), in whom no Mendelian disorders were clinically suspected. In five patients we detect a primary immunodeficiency or enteropathy, with clinical consequences (XIAP, CYBA, SH2D1A, PCSK1). We also present a case study of a VEO-IBD patient with a mosaic de novo, pathogenic allele in CYBB. The mutation is present in ~70% of phagocytes and sufficient to result in defective bacterial handling but not life-threatening infections. Finally, we show that VEO-IBD patients have, on average, higher IBD polygenic risk scores than population controls (99 patients and 18,780 controls; P < 4 × 10−10), and replicate this finding in an independent cohort of VEO-IBD cases and controls (117 patients and 2,603 controls; P < 5 × 10−10). This discovery indicates that a polygenic component operates in VEO-IBD pathogenesis
- …