17 research outputs found
Sulforaphane as an inducer of glutathione prevents oxidative stress-induced cell death in a dopaminergic-like neuroblatoma cell line.
The total GSH depletion observed in the substantia nigra (SN)
appears to be responsible for subsequent oxidative stress
(OS), mitochondrial dysfunction, and dopaminergic cell loss in
patients with Parkinson\u2019s disease. A strategy to prevent the
OS of dopaminergic cells in the SN may be the use of
chemopreventive agents as inducers of endogenous GSH,
antioxidant and phase 2 enzymes. In this study, we demonstrated
that treatment of the dopaminergic-like neuroblastoma
SH-SY5Y cell line with sulforaphane (SF), a cruciferous vegetables
inducer, resulted in significant increases of total GSH
level, NAD(P)H : quinone oxidoreductase-1, GSH-transferase
and -reductase, but not GSH-peroxidase, catalase and
superoxide dismutase activities. Further, the elevation of GSH
levels, GSH-transferase and NAD(P)H:quinone oxidoreductase-
1 activities was correlated to an increase of the resistance
of SH-SY5Y cells to toxicity induced by H2O2 or 6-
hydroxydopamine (6-OHDA). The pre-treatment of SH-SY5Y
cells with SF was also shown to prevent various apoptotic
events (mitochondrial depolarization, caspase 9 and 3 activation
and DNA fragmentation) and necrosis elicited by 6-
OHDA. Further, the impairment of antioxidant capacity and
reactive oxygen species formation at intracellular level after
exposure to 6-OHDA was effectively counteracted by pretreatment
with SF. Last, both the cytoprotective and antioxidant
effects of SF were abolished by the addition of buthionine
sulfoximine supporting the main role of GSH in the neuroprotective
effects displayed by SF. These findings show that
SF may play a role in preventing Parkinson\u2019s disease