Morphological and Quantitative traits of phylogenetic relationships of some barley (Hordeum vulgare L.) accessions in Egypt

phylogenetic relationships of eleven accessions of (Hordeum vulgare L.) collected from different region of Egypt were assessed. Fifteen quantitative morphological traits were used, the measured data were evaluated statistically using ANOVA, phylogenetic tree were constructed using UPGMA. Also Inter simple sequence repeat (ISSR) molecular marker technique were used for DNA fingerprinting and assessing genetic diversity and phylogenetic relationships in barley germplasm. The results showed that ISSR primers produced 140 bands their size ranged between 110-1600 bp with 39.8% polymorphism percentage. Polymorphic information content PIC was 0.74 for ISSR. UPGMA dendrogram was divided into two clusters by morphological traits and ISSR analysis. Genetic similarity matrix was examined with Jacard’s coefficient, maximum similarity was found between B8 and B7 (98%) with morphological analysis both from (North Sinai) and between B9 and B10 (96%) with ISSR analysis. Determination of genetic diversity between barley is of major importance for characterization of barley germplasm, breeding programs and conservation purposes. Morphological traits and ISSR analysis are effective tools for detecting genetic variations. The results showed that H. vulgare have high ratio of variation. This study may be considered as reference study for further studies on H. vulgare and may contribute to species concept and breeding programs

RT-PCR FOR ANTIOXIDANT GENES FROM EGYPTIAN GRAY MANGROVE Avicennia marina UNDER SALT STRESS TO NABQ PROTECTED AREA

RT-PCR was conducted for four genes implicated for salt tolerance, oxidative and osmotic stresses in Egyptian gray mangroves within Nabq protected area in South Sinai Governorate. The results showed over-expression of the mRNA of ferritin (amFer1) gene as very high expression, followed by increase in  mRNA of superoxide dismutase (amSOD1) and ubiquitin conjugation2 (amUBC2).  At the same time gene expression of catalase (amCAT1) decreased

Influence of Methomyl (Copter 90%) on certain biochemical activities and histological structures of land snails Monacha cartusiana

This manuscript was conducted to spotlight the toxic effect of two sub-lethal concentrations of Methomyl (Copter) LC20 (0.075 g/L) and LC40 (0.180 g/L) on some biochemical parameters and histological alterations for land snail Monacha Cartusiana (Muller, 1774). Land snails belong to the class Gastropoda and Phylum Mollusca. This study cleared that both the used concentrations (of Copter) caused a significant increase for activities of three enzymes: alkaline phosphatase (ALP), alanine amino transaminase (ALT), and Aspartate amino transaminase (AST) after 24, 48, and 72 h from exposure starting. In contrast, a total protein (TP) activity decreased at exposure for two concentrations at all lethality periods. Both concentrations of Copter (0.0.75 g/L and 0.180 g/L) have shown histological changes for land snail tissues after 96 h of exposure; digestive gland, hermaphrodite gland, foot, and mantle. Degeneration, rupture, and vacuolization for digestive cells have been shown; furthermore, hemolytic infiltration in connective tissue will be recognized for the digestive gland. The Oocyte and sperm show degenerated with deformation in the connective tissue of the hermaphrodite gland. Likewise, deformation in the muscle fiber layer of the foot in the land snail distorts the epidermis and mucus gland suffering from necrosis. Moreover, mantle shows rapture in epidermis layer, deformed in muscle fiber layer, and vacuolization and necrosis take place in mucus gland. © 2021 The Author(s

Oxygenated Cembrene Diterpenes from Sarcophyton convolutum: Cytotoxic Sarcoconvolutum A–E

The soft coral genus Sarcophyton contains the enzymatic machinery to synthesize a multitude of cembrene-type diterpenes. Herein, highly oxygenated cembrenoids, sarcoconvolutum A–E (1–5) were purified and characterized from an ethyl acetate extract of the red sea soft coral, Sarcophyton convolutum. Compounds were assemblies according to spectroscopic methods including FTIR, 1D- and 2D-NMR as well as HRMS. Metabolite cytotoxicity was tested against lung adenocarcinoma, cervical cancer, and oral-cavity carcinoma (A549, HeLa and HSC-2, respectively). The most cytotoxic compound, (4) was observed to be active against cell lines A549 and HSC-2 with IC50 values of 49.70 and 53.17 μM, respectively

Sarcoconvolutums F and G: Polyoxygenated Cembrane-Type Diterpenoids from <i>Sarcophyton convolutum</i>, a Red Sea Soft Coral

Natural products and chemical analogues are widely used in drug discovery, notably in cancer and infectious disease pharmacotherapy. Sarcophyton convolutum (Alcyoniidae) a Red Sea–derived soft coral has been shown to be a rich source of macrocyclic diterpenes and cyclized derivatives. Two previously undescribed polyoxygenated cembrane-type diterpenoids, sarcoconvolutums F (1) and G (2), as well as four identified analogues (3–6) together with a furan derivate (7) were isolated from a solvent extract. Compounds were identified by spectroscopic techniques, including NMR, HREIMS, and CD, together with close spectral comparisons of previously published data. Sarcoconvolutum F (1) contains a rare 1-peroxid-15-hydroxy-10-ene functionality. Isolated metabolites (1–7) were screened against lung adenocarcinoma (A549), cervical cancer (HeLa) and oral cavity carcinoma (HSC-2) lines. Compound 4 exhibited an IC50 56 µM and 55 µM against A549 and HSC-2 cells, respectively