3 research outputs found
Heterofunctionalized Carbosilane Dendritic Systems: Bifunctionalized Dendrons as Building Blocks versus Statistically Decorated Dendrimers
“Click”
chemistry based on thiol–ene synthetic
protocols has been used to successfully bifunctionalize anionic carbosilane
dendritic structures in two effective ways. The first consists of
the formation of statistically heterofunctionalized dendrimers, the
second being the synthesis of heterofunctionalized dendrons. Regarding
the latter approach, a versatile and simple procedure has been developed
for the synthesis of a library of anionic carbosilane dendrons. Taking
into account the chemical diversity at the focal point for both neutral-
and anionic-terminated dendrons, such as azide, alcohol, amine, bromine,
carboxylic acid, and alkyne, these dendritic systems may act as building
blocks to give more original dendritic architectures
Thiol-Ene Synthesis of Cationic Carbosilane Dendrons: a New Family of Synthons
A variety
of neutral and cationic carbosilane dendrons containing
a wide range of active groups, such as −N<sub>3</sub>, −OH,
−NH<sub>2</sub>, and −SH, at the focal points were synthesized
from carbosilane vinyl dendrons BrG<sub><i>n</i></sub>V<sub><i>m</i></sub> from generations 1–3 by substitution
of the bromine atom at the focal point and functionalization of the
vinyl groups via thiol-ene click chemistry with HS(CH<sub>2</sub>)<sub>2</sub>NMe<sub>2</sub>·HCl. These dendritic wedges were characterized
by NMR, MS, and elemental analysis
Carbosilane Dendrimer 2G-NN16 Represses Tc17 Differentiation in Primary T CD8+ Lymphocytes
We studied changes in gene expression induced by the
carbosilane
dendrimer 2G-NN16 to evaluate their potential as a vehicle for gene
therapy and as medication. Global gene expression profiles on CD8+
T lymphocytes reveal that ribosomal proteins are induced in the presence
of 2G-NN16. IL17A and IL17F, the principal interleukins secreted by
Tc17 cells, a subset of CD8+ T lymphocytes, were down-regulated when
cultured in the presence of this dendrimer. Microarray results were
confirmed by real time quantitative reverse transcriptase polymerase
chain reaction (qRT-PCR). 2G-NN16 also showed a high potential for
in vitro inhibition of Tc17 differentiation of CD8+ T lymphocytes
in the presence of the Tc17 differentiation molecules IL6 and TGF-B1.
These findings suggest that 2G-NN16 could facilitate drug delivery
and may be used to treat inflammatory processes driven by Tc17 cells