29 research outputs found
Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout
Driven Assembly of Lignin into Microcapsules for Storage and Delivery of Hydrophobic Molecules
Oil-filled microcapsules of kraft lignin were synthe-
sized by first creating an oil in water emulsion followed by a high-
intensity, ultrasound-assisted cross-linking of lignin at the water/oil
interface. The rationale behind our approach is based on promoting
documented lignin hydrophobic interactions within the oil phase,
followed by locking the resulting spherical microsystems by covalent
cross-linking using a high intensity ultrasound treatment. As further
evidence in support of our rationale, confocal and optical
microscopies demonstrated the uniformly spherical morphology of
the created lignin microparticles. The detailed elucidation of the
cross-linking processes was carried out using gel permeation
chromatography (GPC) and quantitative 31P NMR analyses. The
ability of lignin microcapsules to incorporate and release Coumarin-6
was evaluated in detail. In vitro studies and confocal laser scanning microscopy analysis were carried out to assess the internalization of capsules into Chinese hamster ovary (CHO) cells. This part of our work demonstrated that the lignin microcapsules are not cytotoxic and readily incorporated in the CHO cells
mRNA expression of genes involved in inflammation and haemostasis in equine fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen and thrombin
Supercritical fluid extraction-high performance liquid chromatography on-line coupling: extraction of some model aromatic compounds
An SFE-HPLC system has been tested with a simple model aromatic mixture on spiked silica. The spiked silica was extracted with pure CO2 and the extract retained in a trap packed with C18 material. Quantitative separation and reproducity have been investigated and are reported
Supercritical fluid extraction-high performance liquid chromatography on-line coupling: Extraction of some model aromatic compounds
Supercritical fluid extraction-high performance liquid chromatography on-line coupling: Extraction of some model aromatic compounds
Preconcentration of environmental trace elements on organic support for neutron activation and high resolution photon spectrometry
Insulin-like growth factor II mRNA binding protein 3 (IMP3) is overexpressed in prostate cancer and correlates with higher Gleason scores
BACKGROUND: The oncofetal protein insulin-like growth factor II mRNA binding protein 3 (IMP3) is an important factor for cell-migration and adhesion in malignancies. Recent studies have shown a remarkable overexpression of IMP3 in different human malignant neoplasms and also revealed it as an important prognostic marker in some tumor entities. To our knowledge, IMP3 expression has not been investigated in prostate carcinomas so far. METHODS: Immunohistochemical stainings for IMP3 were performed on tissue microarray (TMA) organized samples from 507 patients: 31 normal prostate tissues, 425 primary carcinomas and 51 prostate cancer metastases or castration-resistant prostate cancers (CRPC). IMP3 immunoreactivity was semiquantitatively scored and correlated with clinical-pathologic parameters including survival. RESULTS: IMP3 is significantly stronger expressed in prostate carcinomas compared to normal prostate tissues (p < 0.0001), but did not show significant correlation with the pT-stage, the proliferation index (MIB1), preoperative serum PSA level and the margin status. Only a weak and slightly significant correlation was found with the Gleason score and IMP3 expression failed to show prognostic significance in clinico-pathological correlation-analyses. CONCLUSIONS: Although IMP3 is overexpressed in a significant proportion of prostate cancer cases, which might be of importance for novel therapeutic approaches, it does not appear to possess any immediate diagnostic or prognostic value, limiting its potential as a tissue biomarker for prostate cancer. These results might be corroborated by the fact, that two independent tumor cohorts were separately reviewed