活性炭処理豚卵胞液連続投与に伴う家兎卵巣の組織学的変化について

活性炭で処理した豚卵胞液(CTPFF)の投与後からの時間経過に伴ってhCGを投与し、その時間経過が家兎の卵巣組織に及ぼす影響について検討した 1. CTPFFを4mℓずつ12時間おきに10回避統投与し、その直後に開腹して得た卵巣を組織学的に観察したところ、閉鎖進行中の卵胞が目立ち、胞状卵胞数中に占める正常卵胞数の割合は対照の生理的食塩水投与区に比べ有意に低かった 2. 前項1と同様にCTPFFを投与し、投与終了直後(0F-区)、12時間後(12-区)、36時間後(36-区)、96時間後(96-区)にhCG15IUを静注し、4日目に開腹して得た卵巣を組織学的に観察した。0F-区は対照区(生理的食塩水投与直後にhCGを静注して4日目に開腹;0S-区)に比べて胞状卵胞数中に占める正常卵胞数の漁合はわずかに低かったが有意差は認められず、その他の区と比べると有意に低い値を示した。間質細胞の空胞化が全区でところどころで観察されたが、特に0F-区では、脂肪変性様の変性、核変性、細胞崩壊を呈する著しい変化が認められ、卵巣間質全体に散在する空胞化のものが著しく多く、その他に細胞質の染色性が劣って白色化した間質細胞が塊を形成して出現している2種類が確認された。しかし排卵させなかった(黄体形成がない)0F-区の卵巣ではこのような変化は見られなかった。発達した黄体細胞数は、0F、12、36-区では 0S-区に比べて有意に少なく、0F、12-区では黄体組織内に多くの繊維芽細胞様細胞の侵入が認められ、血管の形成不全も観察された。 以上の結果から、CTPFFの連続投与が、家兎卵厳の胞状卵胞の発達を抑制すること、hCGの静注による黄体形成に伴い間質細胞に著しい変性をもたらすこと、黄体形成不全を誘起することが示唆され、これらの影響はCTPFF投与後36時間までに回復しているものと推察された。Mature rabbits were investigated to examine the changes in ovarian histology after successive administrations of charcoal-treated porcine follicular fluid (CTPFF). In experiment 1, treatment groups were injected with CTPFF 10 times at 12-hour intervals. Control group received saline in the same manner as the treatment group. In experiment 2, four groups were injected with CTPFF in the same manner as in experiment 1, and each group received hCG 0 (simultaneously with final injection of CTPFF), 12, 36 and 96 hours after the final injection of CTPFF, respectively. One group received saline and hCG simultaneously with the final injection of saline. All does were laparotomized 4 days after hCG injection. In ovaries of does which had received CTPFF treatment, the mean ratio of number of healthy large antral follicles to total number of all large antral follicles (H/T) was significantly lower than in controls. In ovaries of does which received hCG simultanuously with the final injection of CTPFF (experiment 2), the H/T was lower than that of any other groups, and was significantly lower than the H/T in groups which received hCG 12, 36, and 96 hours after the final administration of CTPFF. Interstitial cells with cytoplasmic vacuolation were found in all ovulated does to some degree. However, degeneration and destruction of interstitial cells were markedly prominent only in does receiving hCG simultanously with the final administration of CTPFF (Group 0F). No vacuolation in interstitial cells was evident in non-ovulatory does. I11 does receiving hCG simultanously with or 12 and 36 hours after CTPFF treatment, number of luteal cells was significantly fewer than in controls. In does which received hCG simultanously with or 12 hours after CTPFF treatment, fibroblast-like cells appeared frequently among luteal tissues. These results indicate that successive administrations of CTPFF give rise to defects in ovarian morphology directly or indirectly in rabbits. It is suggested that these suppressive effects have been restored within 36 hours after the final injection of CTPFF

活性炭処理豚卵胞液投与後の時間経過が, 家兎の排卵数及びプロジェステロン濃度に及ぼす影響

活性炭で処理した豚卵胞液(CTPFF)を家兎に授与し、投与終了時より時間を追ってhCGによる排卵の誘起を試み、それが家兎の排卵数および血漿プロジェステロン濃度に与える影響について検討した 1. CTPFFを4mℓずづ12時間おきに10回連続皮下投与し、その直後に開腹して卵巣表面を観察すると、大型卵胞(≥2.Omm直径)が全く存在しなかったが、対照の生理的食塩水投与区には多数存在していた。 2. 前項1と同様にCTPFFを投与し、投与終了直後(0F-区)、12時間後(12-区)、24時間後(24-区)、36時間後(36-区)、48時間後(48-区)、72時間後(72-区)、96時間後(96-区)の各期にhCG15IUを投与し、4日目に開腹して卵巣表面を観察した。0F-区の排卵数(2.3個)はその他の処理区(6.7～12.0個)及び0S-区(10.2個)と比較して有意に少なかった。平均排卵数は12-区(6.7個)で0S-区(10.2個)より有意に少なかったのに対し、36-区(12.0個)で最も多く(最高18個)、その他の区ではほとんどかわらなかった。また、12-区には大型卵胞(1.5mm≥2.0mm直径)が多く存在していた。末梢血漿中のプロジェステロン濃度(RIA法)はCTPFF処理中は対照区よりも僅かに高かった。12-区はhCG投与後からプロジェステロン濃度が高くなったが、その他の区ではhCG投与後に一度低下し、以後増加した。また、24～72-区はhCG投与4時間後に高い値を示した。外陰部の腫脹・潮紅の度合にはCTPFF投与翌日から低下し、投与終了後に少し回復した。 以上の結果、CTPFFの連続投与が家兎の排卵に対する抑制的影響は比較的早く失われ、CTPFF投与終了後36時間までにFSH濃度にリバウンドが起きていることが推測された。また、プロジェステロン濃度は処理区で高く、ステロイドの生合成に何らかの変化が起きているものと推測された。Effects of treatments for induced ovulation with hCG during 96 hours after successive administrations of charcoal-treated porcine follicular fluid (CTPFF) on the ovulation rate and plasma concentration of progesterone (P) in rabbits were examined. In experiment 1, four does or three does which were injected with 4 ml of CTPFF or saline (control), respectively, at 12-hour intervals for 5 days (10 times) showed no difference in the total number of follices. No large follicles (≥ 2.0 mm in diameter) appeared in ovaries of the treated group, in comparison with their presence in those of the control. In experiment 2, forty-two does were injected with 4 ml of CTPFF in the same manner as in experiment 1. Thirty-six of them were divided into 6 groups according to the number of hours from the final administration of CTPFF to hCG injection-12, 24, 36, 48, 72 and 96 hours. The number of ovulations in six other does which were ovulated by injection of hCG simultaneously with the final administration of CTPFF (group 0F) were significantly lower than those of any other group, and the number increased with interval up to 36 hours from the end of CTPFF treatment to hCG injection. The number of follicles of large size (≥ 2.0 mm in diamter) in ovaries of does in group 12 (12-hour interval) at 4 days after hCG injection was larger than in any other treatment group. In does of groups 24, 36, 48 and 72, plasma P levels 4 hours after hCG injection were higher than those of the other groups of does. In group 12, P levels at 4 days after hCG injection were higher than those of any other group. Vulval swelling and coloration receded 1 day after the beginning of CTPFF treatment, and recovered slightly after the end of CTPFF treatment until hCG injection. These results indicate that successive treatments of CTPFF for 5 days during the follicular phase decrease ovulation rate, and the inhibitory effect on ovulation is lost within a short time. Follicle-stimulating hormone levels may rebound within 36 hours after the final administration of CTPFF treatment, because the ovulation rate in group 36 was the highest