A Novel Approach by SPME-GC/MS for the Determination of gammahydroxybutyric acid (GHB) in Urine Samples after Conversion into gamma-butyrolactone (GBL)

The quantitative determination of gamma-hydroxybutyric acid (GHB) in urine samples is very important to assess illicit intake or administration. To this end we evaluated several analytical methods: headspace gas-chromatography coupled to flame ionization detection (HS-GC/FID), headspace gas-chromatography coupled to mass spectrometry (HS-GC/MS), headspace gas-chromatography coupled to solid phase microextraction and mass spectrometry (HS-SPME-GC/MS). All these methods were endowed with a not sufficient sensitivity, and then we moved to solid phase microextraction coupled to gas-chromatography with mass spectrometry detection (SPME-GC/MS). At first, GHB was extracted from urine with an organic solvent and analyzed after derivatization. Under these conditions, however, there was a partial overlapping between the chromatographic peak of GHB and that of urea, also extracted by the organic solvent. Then we decided to change analytical approach and to convert GHB to gamma-butyrolactone (GBL), which is not an endogenous compound. A SPME method was optimized and validated for the determination of GBL. The limit of detection (LOD) of the method resulted to be 0.25 \u3bcg/mL for GBL, corresponding to 0.5 \u3bcg/mL for GHB. The lower limit of quantification (LLOQ) was 0.4 \u3bcg/mL for GBL and 0.8 \u3bcg/mL for GHB. The LLOQ of the method resulted 10 times lower than the endogenous level, thus allowing to distinguish between physiological conditions and exogenous assumption

Making electromagnetic wavelets

Electromagnetic wavelets are constructed using scalar wavelets as superpotentials, together with an appropriate polarization. It is shown that oblate spheroidal antennas, which are ideal for their production and reception, can be made by deforming and merging two branch cuts. This determines a unique field on the interior of the spheroid which gives the boundary conditions for the surface charge-current density necessary to radiate the wavelets. These sources are computed, including the impulse response of the antenna.Comment: 29 pages, 4 figures; minor corrections and addition

Twisted mass lattice QCD with non-degenerate quark masses

Quantum Chromodynamics on a lattice with Wilson fermions and a chirally twisted mass term is considered in the framework of chiral perturbation theory. For two and three numbers of quark flavours, respectively, with non-degenerate quark masses the pseudoscalar meson masses and decay constants are calculated in next-to-leading order including lattice effects quadratic in the lattice spacing a.Comment: 9 pages, LaTeX2e, reference adde

Chiral perturbation theory for partially quenched twisted mass lattice QCD

Partially quenched Quantum Chromodynamics with Wilson fermions on a lattice is considered in the framework of chiral perturbation theory. Two degenerate quark flavours are associated with a chirally twisted mass term. The pion masses and decay constants are calculated in next-to-leading order including terms linear in the lattice spacing $a$.Comment: 7 pages, LaTeX2e, final published versio

Chiral perturbation theory for lattice QCD with a twisted mass term

Quantum Chromodynamics on a lattice with Wilson fermions and a chirally twisted mass term for two degenerate quark flavours is considered in the framework of chiral perturbation theory. The pion mass and decay constant are calculated in next-to-leading order including terms linear in the lattice spacing $a$.Comment: 5 pages, LaTeX2e, typos corrected, 2 references adde

Further Results on Size and Power of Heteroskedasticity and Autocorrelation Robust Tests, with an Application to Trend Testing

We complement the theory developed in Preinerstorfer and P\"otscher (2016) with further finite sample results on size and power of heteroskedasticity and autocorrelation robust tests. These allows us, in particular, to show that the sufficient conditions for the existence of size-controlling critical values recently obtained in P\"otscher and Preinerstorfer (2018) are often also necessary. We furthermore apply the results obtained to tests for hypotheses on deterministic trends in stationary time series regressions, and find that many tests currently used are strongly size-distorted.Comment: Revised version. Some restructuring, some errors corrected, new results adde

Rhesus monkeys use geometric and non geometric during a reorientation task

Rhesus monkeys (Macaca mulata) were subjected to a place finding task in a rectangular room perfectly homogeneous and without distinctive featural information. Results of Experiment 1 show that monkeys rely on the large-scale geometry of the room to retrieve a food reward. Experiments 2 and 3 indicate that subjects use also nongeometric information (colored wall) to reorient. Data of Experiments 4 and 5 suggest that monkeys do not use small angular cues but that they are sensitive to the size of the cues (Experiments 6, 7, and 8). Our findings strengthen the idea that a mechanism based on the geometry of the environment is at work in several mammalian species. In addition, the present data offer new perspectives on spatial cognition in animals that are phylogenetically close to humans. Specifically, the joint use of both geometric and landmark-based cues by rhesus monkeys tends to demonstrate that spatial processing became more flexible with evolutio