12,766 research outputs found
Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.
DNA strand exchange by serine recombinases has been proposed to occur by a large-scale rotation of halves of the recombinase tetramer. Here we provide the first direct physical evidence for the subunit rotation mechanism for the Hin serine invertase. Single-DNA looping assays using an activated mutant (Hin-H107Y) reveal specific synapses between two hix sites. Two-DNA "braiding" experiments, where separate DNA molecules carrying a single hix are interwound, show that Hin-H107Y cleaves both hix sites and mediates multi-step rotational relaxation of the interwinding. The variable numbers of rotations in the DNA braid experiments are in accord with data from bulk experiments that follow DNA topological changes accompanying recombination by the hyperactive enzyme. The relatively slow Hin rotation rates, combined with pauses, indicate considerable rotary friction between synapsed subunit pairs. A rotational pausing mechanism intrinsic to serine recombinases is likely to be crucial for DNA ligation and for preventing deleterious DNA rearrangements
Discovery of Four High Proper Motion L Dwarfs, Including a 10 pc L Dwarf at the L/T Transition
We discover four high proper motion L dwarfs by comparing the Wide-field
Infrared Survey Explorer (WISE) to the Two Micron All Sky Survey (2MASS). WISE
J140533.32+835030.5 is an L dwarf at the L/T transition with a proper motion of
0.85+/-0.02" yr^-1, previously overlooked due to its proximity to a bright star
(V=12 mag). From optical spectroscopy we find a spectral type of L8, and from
moderate-resolution J band spectroscopy we find a near-infrared spectral type
of L9. We find WISE J140533.32+835030.5 to have a distance of 9.7+/-1.7 pc,
bringing the number of L dwarfs at the L/T transition within 10 pc from six to
seven. WISE J040137.21+284951.7, WISE J040418.01+412735.6, and WISE
J062442.37+662625.6 are all early L dwarfs within 25 pc, and were classified
using optical and low-resolution near-infrared spectra. WISE
J040418.01+412735.6 is an L2 pec (red) dwarf, a member of the class of
unusually red L dwarfs. We use follow-up optical and low-resolution
near-infrared spectroscopy to classify a previously discovered (Castro & Gizis
2012) fifth object WISEP J060738.65+242953.4 as an (L8 Opt/L9 NIR), confirming
it as an L dwarf at the L/T transition within 10 pc. WISEP J060738.65+242953.4
shows tentative CH_4 in the H band, possibly the result of unresolved binarity
with an early T dwarf, a scenario not supported by binary spectral template
fitting. If WISEP J060738.65+242953.4 is a single object, it represents the
earliest onset of CH_4 in the H band of an L/T transition dwarf in the SpeX
Library. As very late L dwarfs within 10 pc, WISE J140533.32+835030.5 and WISEP
J060738.65+242953.4 will play a vital role in resolving outstanding issues at
the L/T transition.Comment: 45 pages, 12 figures, accepted for publication in Ap
Biological Records Centre Annual Report 2005-2006
The period covered by this report is the first year of a new six-year partnership between CEH and JNCC. For this period, there is increased emphasis on targeted survey, on analysis and interpretation and on communications and outreach. These activities were always part of BRC’s work, but they have been given greater prominence as a result of rapid developments in information technology. Data are increasingly reaching BRC in electronic form, so that the effort of data entry and collation is reduced.
The data, collected by many volunteers and then collated and analysed at BRC, document the changing status and distribution of plants and animals in Britain. Distribution maps are published in atlases and are available via the internet through the NBN Gateway. The effects of change or loss of habitats, the influence of climate change and the consequences of changing water quality are all examples of the environmental factors that affect our biodiversity and which BRC aims to document and understand. The results are vital for developing environmental policies, to support conservation, and for fundamental ecological research.
BRC is funded jointly by JNCC and NERC through a partnership based on a Memorandum of Agreement (MoA). The partnership started in 1973 when the Nature Conservancy was divided to form the successor bodies Nature Conservancy Council (NCC) and Institute of Terrestrial Ecology (ITE). NCC was in turn divided further to form JNCC and three Country Agencies, while ITE was merged with other NERC units to form CEH. Through all these changes, the partnership has been maintained. A six-year memorandum of agreement ended on 31 January 2005 (Hill et al. 2005). The present report covers the first full year, 2005-6, of the new agreement for 2005-2010.
Rapid progress in information technology continues to be highly beneficial for BRC, whose data are increasingly used by the UK country conservation agencies, environmental consultants, NGOs, research workers, policy makers and volunteers. It is gratifying to know that, through our ability to display data on the National Biodiversity Network (NBN) Gateway, some of our data suppliers now have immediate access to their own data in a convenient form.
The year 2005-6 has been one of steady progress, with new datasets added to BRC, substantial additions to existing data, and improved communication with the NBN Gateway. The most high profile activity of the year has been the Harlequin Ladybird Survey, which has enabled us to observe the early stages of colonization by a mobile insect in greater detail than has been possible in any previous case
High Resolution Infrared Imaging and Spectroscopy of the Pistol Nebula: Evidence for Ejection
We present new NICMOS/HST infrared images and CGS4/UKIRT Br-alpha (4.05 um)
spectroscopy of the Pistol Star and its associated nebula, finding strong
evidence to support the hypothesis that the Pistol Nebula was ejected from the
Pistol Star. The Pa-alpha NICMOS image shows that the nebula completely
surrounds the Pistol Star, although the line intensity is much stronger on its
northern and western edges. The Br-alpha spectra show the classical ring-like
signature of quasi-spherical expansion, with weak blueshifted emission (V_max
approx -60 km/s) and strong redshifted emission (V_max approx +10 km/s), where
the velocities are with respect to the velocity of the Pistol Star; further,
the redshifted emission appears to be "flattened" in the position-velocity
diagram. These data suggest that the nebula was ejected from the star several
thousand years ago, with a velocity between the current terminal velocity of
the stellar wind (95 km/s) and the present expansion velocity of gas in the
outer shell of the nebula (60 km/s). The Pa-alpha image reveals several
emission-line stars in the region, including two newly-identified emission-line
stars north of the Pistol Star with spectral types earlier than WC8 (T_eff >
50,000 K). The presence of these stars, the morphology of the Pa-alpha
emission, and the velocity field in the gas suggest that the side of the nebula
furthest from us is approaching, and being ionized by, the hot stars of the
Quintuplet, and that the highest velocity redshifted gas has been decelerated
by winds from the Quintuplet stars. We also discuss the possibility that the
nebular gas might be magnetically confined by the ambient magnetic field
delineated by the nearby nonthermal filaments.Comment: Figure 1 is included as a JPG file. Figure 1 and 2 also available at
ftp://quintup.astro.ucla.edu/pistol2
Recent Advances in Smellscape Research for the Built Environment
The interrelationships between humans, smells and the built environment have been the focus of increasing numbers of research studies in the past ten years. This paper reviews these trends and identifies the challenges in smellscape research from three aspects: methodological approaches, artistic design interventions and museum practices, and odour policy making. In response to the gaps and challenges identified, three areas of future research have also been identified for this field: smell archives and databases, social justice within odour control and management, and research into advanced building materials
Acute Respiratory Infections In Civilian Adults II. Clinical And Serologic Observations In A Vaccine Study Group
The Evolution of the Stellar Hosts of Radio Galaxies
We present new near-infrared images of z>0.8 radio galaxies from the
flux-limited 7C-III sample of radio sources for which we have recently obtained
almost complete spectroscopic redshifts. The 7C objects have radio luminosities
about 20 times fainter than 3C radio galaxies at a given redshift. The absolute
magnitudes of the underlying host galaxies and their scale sizes are only
weakly dependent on radio luminosity. Radio galaxy hosts at z~2 are
significantly brighter than the hosts of radio-quiet quasars at similar
redshifts and the model AGN hosts of Kauffmann & Haehnelt (2000). There is no
evidence for strong evolution in scale size, which shows a large scatter at all
redshifts. The hosts brighten significantly with redshift, consistent with the
passive evolution of a stellar population that formed at z>~3. This scenario is
consistent with studies of host galaxy morphology and submillimeter continuum
emission, both of which show strong evolution at z>~2.5. The lack of a strong
``redshift cutoff'' in the radio luminosity function to z>4 suggests that the
formation epoch of the radio galaxy host population lasts >~1Gyr from z>~5 to
z~3. We suggest these facts are best explained by models in which the most
massive galaxies and their associated AGN form early due to high baryon
densities in the centres of their dark matter haloes.Comment: To appear in A
Strong Nebular Line Ratios in the Spectra of z~2-3 Star-forming Galaxies: First Results from KBSS-MOSFIRE
We present initial results of a deep near-IR spectroscopic survey covering
the 15 fields of the Keck Baryonic Structure Survey (KBSS) using MOSFIRE on the
Keck 1 telescope, focusing on a sample of 251 galaxies with redshifts 2.0< z <
2.6, star-formation rates 2 < SFR < 200 M_sun/yr, and stellar masses 8.6 <
log(M*/M_sun) < 11.4, with high-quality spectra in both H- and K-band
atmospheric windows. We show unambiguously that the locus of z~2.3 galaxies in
the "BPT" nebular diagnostic diagram exhibits a disjoint, yet similarly tight,
relationship between the ratios [NII]6585/Halpha and [OIII]/Hbeta as compared
to local galaxies. Using photoionization models, we argue that the offset of
the z~2.3 locus relative to z~ 0 is explained by a combination of harder
ionizing radiation field, higher ionization parameter, and higher N/O at a
given O/H than applies to most local galaxies, and that the position of a
galaxy along the z~2.3 star-forming BPT locus is surprisingly insensitive to
gas-phase oxygen abundance. The observed nebular emission line ratios are most
easily reproduced by models in which the net ionizing radiation field resembles
a blackbody with effective temperature T_eff = 50000-60000 K and N/O close to
the solar value at all O/H. We critically assess the applicability of
commonly-used strong line indices for estimating gas-phase metallicities, and
consider the implications of the small intrinsic scatter in the empirical
relationship between excitation-sensitive line indices and stellar mass (i.e.,
the "mass-metallicity" relation), at z~2.3.Comment: 41 pages, 25 figures, accepted for publication in the Astrophysical
Journal. Version with full-resolution figures available at
http://www.astro.caltech.edu/~ccs/mos_bpt_submit.pd
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