Affinity and selectivity of plant proteins for red wine components relevant to color and aroma traits

The effects of fining with various plant proteins were assessed on Aglianico red wine, using both the young wine and wine aged for twelve and twenty-four months, and including wine unfined or fined with gelatin as controls. Color traits and fining efficiency were considered, along with the content of various types of phenolics and of aroma-related compounds of either varietal or fermentative origin. All agents had comparable fining efficiency, although with distinct kinetics, and had similar effects on wine color. Individual plant proteins and enzymatic hydrolyzates differed in their ability to interact with some anthocyanins, with specific proanthocyanidins complexes, and with some aroma components of fermentative origin. Changes in varietal aroma components upon fining were very limited or absent. Effects of all the fining agents tested in this study on the anthocyanidin components were most noticeable in young red wine, and decreased markedly with increasing wine ageing

Automation in public sector jobs and services: a framework to analyze public digital transformation's impact in a data-constrained environment

PurposeThis study aims to estimate the value of the impact from digital transformation (DX) focusing on its automation effect, looking at the time and cost savings coming from the substitution effect with an adoption of digital technologies. For example, cloud and artificial intelligence technologies such as ChatGPT have the potential to change ways of working, substituting and replacing several of the tasks that are currently carried out by public administration (PA) employees and labor processes underpinning PA services.Design/methodology/approachThe paper outlines a new framework to estimate the potential impact of DX on the public sector. The authors apply this framework to estimate the value of the impact of DX on the Italian PA, defining the latter by the collection of the value of its labor (i.e. PA workforce salaries) and by the collection of the value of its outputs (i.e. public services' costs).FindingsThis study ultimately maps out the magnitude and trends of how likely the PA occupations and services could be substituted in a wider process of DX. To do this, the authors apply their framework to the Italian PA, and they triangulate secondary data collection, from official accounts of the Italian Ministry of Economics and the National Statistical Institute, with methodological antecedents from the UK Office for National Statistics and experts' insights. Results provide a snapshot on the type and magnitude of PA jobs and services projected to be affected by automation over the next 10 years.Originality/valueTo the best of the authors' knowledge, this paper provides for the first time an approach to estimate the value of the impact of DX on the public sector in a data-constrained environment - or in the lack of the required primary data. Once applied to the Italian PA, this approach provides a granular map of the automatability of each of the PA occupations and of the PA services. Finally, this paper mentions preliminary insights on potential challenges related to equity in public sector jobs and implications on recruitment processes

TrustShadow: Secure Execution of Unmodified Applications with ARM TrustZone

The rapid evolution of Internet-of-Things (IoT) technologies has led to an emerging need to make it smarter. A variety of applications now run simultaneously on an ARM-based processor. For example, devices on the edge of the Internet are provided with higher horsepower to be entrusted with storing, processing and analyzing data collected from IoT devices. This significantly improves efficiency and reduces the amount of data that needs to be transported to the cloud for data processing, analysis and storage. However, commodity OSes are prone to compromise. Once they are exploited, attackers can access the data on these devices. Since the data stored and processed on the devices can be sensitive, left untackled, this is particularly disconcerting. In this paper, we propose a new system, TrustShadow that shields legacy applications from untrusted OSes. TrustShadow takes advantage of ARM TrustZone technology and partitions resources into the secure and normal worlds. In the secure world, TrustShadow constructs a trusted execution environment for security-critical applications. This trusted environment is maintained by a lightweight runtime system that coordinates the communication between applications and the ordinary OS running in the normal world. The runtime system does not provide system services itself. Rather, it forwards requests for system services to the ordinary OS, and verifies the correctness of the responses. To demonstrate the efficiency of this design, we prototyped TrustShadow on a real chip board with ARM TrustZone support, and evaluated its performance using both microbenchmarks and real-world applications. We showed TrustShadow introduces only negligible overhead to real-world applications.Comment: MobiSys 201

Studies on the mechanism of catalysis of iron-sulfur cluster transfer from IscU[2Fe2S] by HscA/HscB chaperones

The HscA/HscB chaperone/cochaperone system accelerates transfer of iron-sulfur clusters from the FeS-scaffold protein IscU (IscU(2)[2Fe2S], holo-IscU) to acceptor proteins in an ATP-dependent manner. We have employed visible region circular dichroism (CD) measurements to monitor chaperone-catalyzed cluster transfer from holo-IscU to apoferredoxin and to investigate chaperone-induced changes in properties of the IscU(2)[2Fe2S] cluster. HscA-mediated acceleration of [2Fe2S] cluster transfer exhibited an absolute requirement for both HscB and ATP. A mutant form of HscA lacking ATPase activity, HscA(T212V), was unable to accelerate cluster transfer, suggesting that ATP hydrolysis and conformational changes accompanying the ATP (T-state) to ADP (R-state) transition in the HscA chaperone are required for catalysis. Addition of HscA and HscB to IscU(2)[2Fe2S] did not affect the properties of the [2Fe2S] cluster, but subsequent addition of ATP was found to cause a transient change of the visible region CD spectrum, indicating distortion of the IscU-bound cluster. The dependence of the rate of decay of the observed CD change on ATP concentration and the lack of an effect of the HscA(T212V) mutant were consistent with conformational changes in the cluster coupled to ATP hydrolysis by HscA. Experiments carried out under conditions with limiting concentrations of HscA, HscB, and ATP further showed that formation of a 1:1:1 HscA-HscB-IscU(2)[2Fe2S] complex and a single ATP hydrolysis step are sufficient to elicit the full effect of the chaperones on the [2Fe2S] cluster. These results suggest that acceleration of iron-sulfur cluster transfer involves a structural change in the IscU(2)[2Fe2S] complex during the T --> R transition of HscA accompanying ATP hydrolysis