342 research outputs found
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Tales of the unexpected: research discoveries that occurred by accident
Data from four experimental research projects are presented which have in common that unexpected results caused a change in direction of the research. A plant growth accelerator caused the appearance of white black bean aphids, a synthetic pyrethroid suspected of enhancing aphid reproduction proved to enhance plant
growth, a chance conversation with a colleague initiated a search for fungal DNA in aphids, and the accidental
invasion of aphid cultures by a parasitoid reversed the aphid population ranking of two Brussels sprout cultivars. This last result led to a whole series of studies on the plant odour preferences of emerging parasitoids which in turn revealed the unexpected phenomenon that chemical cues to the maternal host plant are left with the eggs at oviposition. It is pointed out that, too often, researchers fail to follow up unexpected results because they resist accepting flaws in their hypotheses; also that current application criteria for research funding make it hard to accommodate unexpected findings
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Integrated pest management of aphids and introduction to IPM case studies
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Plastic films for polytunnels can prolong the effective residual life of cypermethrin to over 6 months
Synthetic pyrethroid insecticides are degraded almost entirely by ultraviolet (UV)-catalysed oxidation. A bioassay using the beetle Tribolium confusum duVal caged on bandages soaked in 0.04% a.i. cypermethrin showed large differences in residual insecticide-life under three plastic films available for cladding polytunnels. Cypermethrin exposed to a UV film that transmitted 70% of UVB and 80% of UVA killed all beetles for 8 weeks, compared to only 3 weeks for cypermethrin exposed in a clear plastic envelope. Cypermethrin under a UV-absorbing film that reduced the transmission of UVB and UVA to 14% and 50%, respectively, gave a complete kill for 17 weeks. Reducing the transmission of UVB to virtually zero, and that of UVA to only 3%, using a UV-opaque film prolonged the effective life of the cypermethrin residue to 26 weeks, and some beetles were still killed for a further 11 weeks. Even after this time, beetles exposed to cypermethrin from the UV-opaque treatment were still affected by the insecticide, and only showed near-normal mobility after 24 months of pesticide exposure to the UV-opaque film. These results have implications for the recommended intervals between cypermethrin treatment and crop harvest, and on the time of introduction of insect-based biological control agents, when UV-opaque films are used in commercial horticulture
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Mechanisms of partial plant resistance to diamondback moth (Plutella xylostella) in brassicas
Artificial diet studies were used to differentiate among physical and chemical mechanisms affecting the suitability to diamondback moth (Plutella xylostella L.), of 16 food substrates obtained by growing four different brassicas in the glasshouse or field and measuring the pest's performance on either leaf discs or a diet incorporating leaf powders. Leaves of Chinese cabbage and the cabbage cultivar 'Minicole' were, respectively, the most and least suitable leaves for the insect, but this ranking was reversed on artificial diet. Leaves of glasshouse-grown plants were more suitable than those of plants grown in the fields. Differences in the suitability of leaves to diamondback moth appeared to be largely determined by leaf toughness and surface wax load. Concentrations of individual glucosinolates in the brassicas probably acted as phagostimulants, so increasing their intrinsic susceptibility to diamondback moth, but the effect of the physical factors appeared more important
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A fully defined artificial diet for Myzus persicae – the detailed technical manual
We have been able to maintain a continuous culture of Myzus persicae (Hemiptera: Aphididae) on a fully defined artificial diet at Reading, UK, for over 30 years, without any return to plants. Following multiple requests from aphid researchers, here we provide the details of our culturing method. Detailed instructions are given for rearing M. persicae on the diet. An improved recipe is included as well as the construction and changing of diet sachets
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Poor survival on an artificial diet of two genotypes of the aphid Myzus persicae: a fitness cost of insecticide resistance?
A fully susceptible genotype (4106A) of Myzus persicae survived the longest on an artificial diet and, in several of the eight replicates, monitoring was terminated when the culture was still thriving. A genotype with elevated carboxylesterase FE4 at the R3 level (800F) had a mean survival of only 98.13 days, whereas 794J, which combines R3 E4 carboxylesterase with target-site resistance (knockdown resistance), survived for the even shorter mean time of 84.38 days.
The poorer survival of the two genotypes with extremely elevated carboxylesterase-resistance was not the result of a reluctance to transfer to new diet at each diet change.
Although available for only two replicates, a revertant clone of 794J (794Jrev), which has the same genotype as 794J but the amplified E4 genes are not expressed leading to a fully susceptible phenotype, did not appear to survive any better than this clone. This suggests that the poor survival on an artificial diet of the extreme-carboxylesterase genotypes is not the result of the cost of over-producing the enzyme.
The frequency of insecticide-resistant genotypes is low in the population until insecticide is applied, indicating that they have reduced fitness, although this does not necessarily reflect a direct cost of expressing the resistance mechanism
Effects of sowing date and insecticides on cereal aphid populations and barley yellow dwarf virus on barley in Kenya
The effects of the date of sowing and insecticide sprays on aphid populations and barley yellow dwarf virus (BYDV) incidence in barley was studied in Mau Narok, Kenya. Rhopalosiphum padi (L.) and Metopolophium dirhodum (WLK.) were common aphid species, but other cereal aphids present were Rhopalosiphum maidis (Fitch), Stiobion avenae (F.), Schizaphis grammum (Rond.) and Hysteroneura setaria Thom. The incidence of BYDV was significantly decreased in plots sown with seed that had been treated with imidacloprid (NTN‐33893, Gaucho) and subsequently sprayed with foliar insecticide (Cypermethrin). Yield loss due to BYDV was also significantly different between the treatments and between the earlyplanted and the late‐planted crop (P < 0.05). Grain yield and 1000‐grain weight were not significantly different among insecticide treatments in the early‐planted crop. In the late‐planted crop, the yield increase with seed treatment alone was highly significant (P < 0.001), with a yield increase of 36‐43%, more than that of the untreated control. Grain yield was significantly (P < 0.05) negatively correlated with the total number of cereal aphids. as well as with the numbers of R. padi alone
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