Polymorphism identification and improved genome annotation of Brassica rapa through Deep RNA sequencing.

The mapping and functional analysis of quantitative traits in Brassica rapa can be greatly improved with the availability of physically positioned, gene-based genetic markers and accurate genome annotation. In this study, deep transcriptome RNA sequencing (RNA-Seq) of Brassica rapa was undertaken with two objectives: SNP detection and improved transcriptome annotation. We performed SNP detection on two varieties that are parents of a mapping population to aid in development of a marker system for this population and subsequent development of high-resolution genetic map. An improved Brassica rapa transcriptome was constructed to detect novel transcripts and to improve the current genome annotation. This is useful for accurate mRNA abundance and detection of expression QTL (eQTLs) in mapping populations. Deep RNA-Seq of two Brassica rapa genotypes-R500 (var. trilocularis, Yellow Sarson) and IMB211 (a rapid cycling variety)-using eight different tissues (root, internode, leaf, petiole, apical meristem, floral meristem, silique, and seedling) grown across three different environments (growth chamber, greenhouse and field) and under two different treatments (simulated sun and simulated shade) generated 2.3 billion high-quality Illumina reads. A total of 330,995 SNPs were identified in transcribed regions between the two genotypes with an average frequency of one SNP in every 200 bases. The deep RNA-Seq reassembled Brassica rapa transcriptome identified 44,239 protein-coding genes. Compared with current gene models of B. rapa, we detected 3537 novel transcripts, 23,754 gene models had structural modifications, and 3655 annotated proteins changed. Gaps in the current genome assembly of B. rapa are highlighted by our identification of 780 unmapped transcripts. All the SNPs, annotations, and predicted transcripts can be viewed at http://phytonetworks.ucdavis.edu/

A Scalable QoS Scheduling Architecture For WiMAX Multi-Hop Relay Networks.

WiMAX Mobile Multi-hop Relay (MMR) network has been introduced to increase the capacity and extend the coverage area of a single WiMAX Base Station (BS) by the use of a Relay Station (RS)

Control and Media Sessions: lAX with RSW Control Criteria

This paper discusses technical issues related to delivery and control of IP multimedia services, such as multimedia conferencing, involving heterogeneous end terminals. In particular, it proposes the design of an experimental system for interworking between InterAsterisk eXchange Protocol and RSW session and call control signaling protocols

Technique de lombriculture au Sud Vietnam

Vermicomposting technique in South Vietnam. Earthworms play a major role in organic matter transformation. The vermicomposting allows to combine several advantages: the management of diversified organic wastes, and the production of earthworms and vermicompost. Crop residues and other plant wastes mixed with animal manure from individual farms can be used. In South Vietnam, farmers are rearing some livestock and growing a few number of crop species. From several years, an increasing number of vermicomposting units were set in many farms from the Ho Chi Minh City region. Two kinds of infrastructure materials are used: baked clay blocks or bamboo stems with plastic covers. In South Vietnam, all conditions are pooled to ensure an efficient earthworm production: suitable climate, available organic wastes and materials to build the vermicomposting structures. Both field plot fertility and protein feed for livestock (pigs, poultry, etc.) can be provided by rearing earthworms

Content-based video retrieval: three example systems from TRECVid

The growth in available online video material over the internet is generally combined with user-assigned tags or content description, which is the mechanism by which we then access such video. However, user-assigned tags have limitations for retrieval and often we want access where the content of the video itself is directly matched against a user’s query rather than against some manually assigned surrogate tag. Content-based video retrieval techniques are not yet scalable enough to allow interactive searching on internet-scale, but the techniques are proving robust and effective for smaller collections. In this paper we show 3 exemplar systems which demonstrate the state of the art in interactive, content-based retrieval of video shots, and these three are just three of the more than 20 systems developed for the 2007 iteration of the annual TRECVid benchmarking activity. The contribution of our paper is to show that retrieving from video using content-based methods is now viable, that it works, and that there are many systems which now do this, such as the three outlined herein. These systems, and others can provide effective search on hundreds of hours of video content and are samples of the kind of content-based search functionality we can expect to see on larger video archives when issues of scale are addressed

Preemptive analgesic effect of intrathecal applications of neuroactive steroids in a rodent model of post-surgical pain: Evidence for the role of T-type calcium channels

Preemptive management of post-incisional pain remains challenging. Here, we examined the role of preemptive use of neuroactive steroids with activity on low-voltage activated T-type C

Menstrual pattern and menstrual disorders among adolescents: an update of the Italian data.

Abstract Background The most striking event in the whole process of female puberty is the onset of menstruation. To our knowledge, no large population-based studies have been performed on the topic of menstrual health among Italian adolescents in recent years. The aims of this study were to produce up-to-date information on the menstrual pattern of Italian girls attending secondary school, and to estimate the prevalence of menstrual cycle abnormalities in this population. Methods This was a cross-sectional study on a population-based sample of Italian adolescents aged 13–21 years attending secondary school. Only girls who had already started menstruating were requested to participate. Information was collected by means of a questionnaire that included items on the girls’ demographic details, anthropometrics, smoking and drinking habits, use of contraceptive pills, and socioeconomic status. The questions on the girls’ menstrual pattern concerned their age at menarche, duration of the most recent menstruation intervals (35 days, variable), average days of bleeding (6 days), and any menstrual problems and their frequency. Results A total of 6,924 questionnaires were administered and 4,992 (71%) were returned. One hundred girls failed to report their date of birth, so 4,892 subjects were analyzed. The girls’ mean age was 17.1 years (SD ±1.4); their mean age at menarche was 12.4 (±1.3) years, median 12.4 years (95%CI 12.3–12.5). In our sample population, 3.0% (95%CI 2.5%-3.4%) of the girls had menstruation intervals of less than 21 days, while it was more than 35 days in 3.4% (95%CI 2.9%-3.9%). About 9% of the girls (95%CI 7.7%-9.4%) said the length of their menstruation interval was currently irregular. Short bleeding periods (6 days) in 19% (95%CI 17.9%-20.1%). Menstruation-related abdominal pain was reported by about 56% of our sample. About 6.2% of the girls (95%CI 5.4%-7.0%) were suffering from dysmenorrhea. Conclusions In conclusion, to the best of our knowledge, this is one of the largest studies on menstrual patterns and menstrual disorders among Italian adolescent girls. Adolescent girls referring persistent oligomenorrhoea, in first two years from menarche, had a higher risk for developing a persistent menstrual irregularity. They had longer bleeding periods (>6 days) and this has practical implications because it makes these adolescents potentially more susceptible to iron deficiency anemia. Clinicians need to identify menstrual abnormalities as early as possible in order to minimize their possible consequences and sequelae, and to promote proper health information. We recommend that adolescents should be encouraged to chart their menstrual frequency and regularity prospectively from the menarche onwards.</p