Microsatellite alteration and immunohistochemical expression profile of chromosome 9p21 in patients with sporadic renal cell carcinoma following surgical resection.

BACKGROUND: Long-term prognostic significance of loss of heterozygosity on chromosome 9p21 for localized renal cell carcinoma following surgery remains unreported. The study assessed the frequency of deletions of different loci of chromosome 9p along with immunohistochemical profile of proteins in surgically resected renal cancer tissue and correlated this with long-term outcomes. METHODS: DNA was extracted from renal tumours and corresponding normal kidney tissues in prospectively collected samples of 108 patients who underwent surgical resection for clinically localized disease between January 2001 and December 2005, providing a minimum of 9 years follow-up for each participant. After checking quality of DNA, amplified by PCR, loss of heterozygosity (LOH) on chromosome 9p was assessed using 6 microsatellite markers in 77 clear cell carcinoma. Only 5 of the markers showed LOH (D9S1814, D9S916, D9S974, D9S942, and D9S171). Protein expression of p15(INK4b), p16(INK4a), p14(ARF), CAIX, and adipose related protein (ADFP) were demonstrated by immunostaining in normal and cancer tissues. Loss of heterozygosity for microsatellite analysis was correlated with tumour characteristics, recurrence free, cancer specific, and overall survival, including significance of immunohistochemical profile of protein expressions. RESULTS: The main deletion was found at loci telomeric to CDKN2A region at D9S916. There was a significant correlation between frequency of LOH stage (p = 0.005) and metastases (p = 0.006) suggesting a higher LOH for advanced and aggressive renal cell carcinoma. Most commonly observed LOH in the 3 markers: D9S916, D9S974, and D9S942 were associated with poor survival, and were statistically significant on multivariate analysis. Immunohistochemical expression of p14, p15, and p16 proteins were either low or absent in cancer tissue compared to normal. CONCLUSIONS: Loss of heterozygosity of p921 chromosome is associated with aggressive tumours, and predicts cancer specific or recurrence free survival on long-term follow-up. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12885-016-2514-8) contains supplementary material, which is available to authorized users

Characterization and therapeutic application of canine adipose mesenchymal stem cells to treat elbow osteoarthritis

Visceral adipose tissue (AT) obtained from surgical waste during routine ovariectomies was used as a source for isolating canine mesenchymal stem cells (MSCs). As determined by cytofluorimetry, passage 2 cells expressed MSC markers CD44 and CD90 and were negative for lineage-specific markers CD34 and CD45. The cells differentiated toward osteogenic, adipogenic, and chondrogenic directions. With therapeutic aims, 30 dogs (39 joints) suffering from elbow dysplasia (ED) and osteoarthritis (OA) were intra-articularly transplanted with allogeneic MSCs suspended in 0.5% hyaluronic acid (HA). A highly significant improvement was achieved without any medication as demonstrated by the degree of lameness during the follow-up period of 1 y. Control arthroscopy of 1 transplanted dog indicated that the cartilage had regenerated. Histological analysis of the cartilage biopsy confirmed that the regenerated cartilage was of hyaline type. These results demonstrate that transplantation of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) is a novel, noninvasive, and highly effective therapeutic tool in treating canine elbow dysplasia

6 alpha particle emission in the reaction N-13+B-11

The six-ct decay of Mg-24 has been studied via the reaction N-13+ B-11 at two beam energies, 29.5 MeV and if 45 MeV. Large detector arrays allowed this reaction to be studied with low intensity radioactive N-13 beam. The excitation energy in the Mg-24* was in the region of the 46 MeV resonance in C-12+C-12 scattering. From the six-a particles, events of interest were selected by associating two of the a particle pairs as the decay products of Be-8. The c.m. energy spectra for two Be-8 + two alpha events have been extracted. The analysis revealed that most of these events originate from C-12(31)- + C-12(31)-. The total cross section for these six-alpha events has been extracted at the two beam energies. [S0556-2813(99)06902-2]

Different aspects of nuclear structure and reaction mechanisms in the collision N-13+B-11

The reaction N-13 + B-11 has been investigated at two 13N beam energies: 29.5 and 45 MeV, Charged particles and gamma rays emitted in the reactions have been detected using a large array of silicon strip detectors and BaF2 scintillators, respectively. Several reaction channels have been analysed with different aims. The proton transfer reaction B-11(N-13, C-12)C-12 to various excited states of C-12 has been studied to obtain information on the N-13(g.s.) structure. The excitation energy of the Mg-24 compound nucleus was in the region where several resonances occur in the C-12 + C-12 system. The six-alpha decay of the Mg-24 intermediate system has been studied. Some of these events have been identified as coming from the break-up of two C-12* in the 3(1)(-)-3(1)(-) and 3(1)(-)-0(2)(+) states. The total fusion cross section has also been extracted from the data. In order to extract information on the isospin purity/mixing in Mg-24 at high excitation energy (E-x similar to 47 MeV), the GDR gamma emission from this self-conjugate nucleus has been studied. A comparison of gamma spectra has been undertaken for the reactions N-13 + B-11 and N-14 + B-10. (C) 2001 Elsevier Science B.V, Ail rights reserved

The transfer reaction B-11(N-13,C-12)C-12

The transfer reaction B-11(N-13,C-12)C-12* has been investigated at the two laboratory energies E-lab = 45 MeV and E-lab = 29.5 MeV. Particle-gamma and particle-particle coincidences have been measured. The coincidence between a heavy ion and the 15.1 MeV gamma-ray emitted from C-12* shows transition to the C-12(gs) + C-12*(15.1) and C-12*(4.44) + C-12*(15.1) exit channels. These indicate the mixed ground-state structure of N-13. DWBA calculations have also been performed. Analysis of particle-particle coincidence data revealed the presence of transfer channels also to unbound states of C-12 such as C-12(gs) + C-12* (E* > 7.64 MeV) and C-12*(4.44) + C-12* (E* >7.64 MeV). The angular distributions have been extracted for these two channels. Six-alpha-particle events have also been analyzed. Some of these events have been identified as coming from B-11(N-13,C-12*)C-12* reaction. An enhancement in the six-alpha cross-section is observed at an excitation energy for the compound nucleus Mg-24 close to the 46 MeV resonances observed in C-12+C-12 inelastic scattering. These six-alphas events are generated mostly from the break-up of C-12(3(1)(-))+(12) C(3(1)(-)) and therefore seems not to be associated with the six-alphas chain state

New limits for the Ne-19(p,gamma)Na-20 astrophysical reaction rate from direct measurements using radioactive beams

Inexplosive stellar hydrogen burning, the hot CNO cycles and the rp-process are mainly linked by the reaction sequence O-15(alpha,gamma) Ne-19(p,gamma)Na-20. Using intense Ne-19 radioactive beams, both the Ne-19(p,gamma) and the Ne-19(d,n) reaction have been studied. Upper and lower limits for the Ne-19(p,gamma) reaction rate have been deduced, allowing to conclude that the O-15(alpha,gamma) reaction is most likely the bottleneck reaction