Integration of environmental assessment in a PLM context: a case study in luxury industry

Nowadays, the environment becomes a major issue in our society. It gives rise to regu-lations, market demand and stakeholder's pressure which are concerning companies. These latter have to reduce the negative impact of their new product by eco-design and adopting a continuous improvement for their existing product portfolio. To do so, environmental assessment system is needed. Life Cycle Assessment (LCA) is the most known and recognized. However, this method is complex, requires significant resources and a large amount of accurate data. We propose a methodology to connect a simplified LCA tool with PLM system and ERP to evaluate an entire product portfolio at any time. This will allow design teams to consider the environmental issues in early design phase and gives the companies a global vision of their product portfolio. This methodology is experimented with packaging products of luxury brand, using the Teamcenter PLM system and a Simplified LCA Too

Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods

Cystic fibrosis (CF) predisposes patients to bacterial colonization and infection of the lower airways. Several species belonging to the genus Burkholderia are potential CF-related pathogens, but microbiological identification may be complicated. This situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic assays is required. A total of 114 geographically diverse bacterial isolates, previously identified in reference laboratories as Burkholderia cepacia (n = 51), B. gladioli (n = 14), Ralstonia pickettii (n = 6), B. multivorans (n = 2), Stenotrophomonas maltophilia (n = 3), and Pseudomonas aeruginosa (n = 11), were collected from environmental, clinical, and reference sources. In addition, 27 clinical isolates putatively identified as Burkholderia spp. were recovered from the sputum of Dutch CF patients. All isolates were used to evaluate the accuracy of two selective growth media, four systems for biochemical identification (API 20NE, Vitek GNI, Vitek NFC, and MicroScan), and three different PCR-based assays. The PCR assays amplify different parts of the ribosomal DNA operon, either alone or in combination with cleavage by various restriction enzymes (PCR-restriction fragment length polymorphism [RFLP] analysis). The best system for the biochemical identification of B. cepacia appeared to be the API 20NE test. None of the biochemical assays successfully grouped the B. gladioli strains. The PCR-RFLP method appeared to be the optimal method for accurate nucleic acid-mediated identification of the different Burkholderia spp. With this method, B. gladioli was also reliably classified in a separate group. For the laboratory diagnosis of B. cepacia, we recommend parallel cultures on blood agar medium and selective agar plates. Further identification of colonies with a Burkholderia phenotype should be performed with the API 20NE test. For final confirmation of species identities, PCR amplification of the small-subunit rRNA gene followed by RFLP analysis with various enzymes is recommended

Gender Differences in Global but Not Targeted Demethylation in iPSC Reprogramming

Global DNA demethylation is an integral part of reprogramming processes in vivo and in vitro, but whether it occurs in the derivation of induced pluripotent stem cells (iPSCs) is not known. Here, we show that iPSC reprogramming involves both global and targeted demethylation, which are separable mechanistically and by their biological outcomes. Cells at intermediate-late stages of reprogramming undergo transient genome-wide demethylation, which is more pronounced in female cells. Global demethylation requires activation-induced cytidine deaminase (AID)-mediated downregulation of UHRF1 protein, and abolishing demethylation leaves thousands of hypermethylated regions in the iPSC genome. Independently of AID and global demethylation, regulatory regions, particularly ESC enhancers and super-enhancers, are specifically targeted for hypomethylation in association with transcription of the pluripotency network. Our results show that global and targeted DNA demethylation are conserved and distinct reprogramming processes, presumably because of their respective roles in epigenetic memory erasure and in the establishment of cell identity.This work was funded by the Wellcome Trust ( 095645/Z/11/Z ), BBSRC ( BB/K010867/1 ), EU NoE Epigenesys, and FEBS (Long-term fellowship to I.M.)

Decrease in antibiotic resistance among invasive pneumococcal disease (IPD) isolates in France from 2003 to 2007; On-going survey of the French Pneumococcus network (ORP)

Date du colloque&nbsp;: 10/2008</p

Epidemiology and Antimicrobial Resistance of Streptococcus pneumoniae in France in 2007: Data from the Pneumococcus Surveillance Network

Antimicrobial resistance of Streptococcus pneumoniae in France is closely monitored by the pneumococcus surveillance network, founded in 1995, which collects data from regional observatories (Observatoire Régionaux du Pneumocoque [ORP]). In 2007, 23 ORPs analyzed the antibiotic susceptibility of 5,302 isolates of S. pneumoniae recovered in France from cerebrospinal fluid, blood, middle ear fluid, and pleural fluid, as well as from adult respiratory samples. The study showed that 38.2% of the strains were nonsusceptible to penicillin, 19.3% nonsusceptible to amoxicillin, and 10.5% nonsusceptible to cefotaxime. The percentage of pneumococcus nonsusceptible to penicillin varied according to both the sample and the age of the patient (child/adult): blood (27.8%/32.5%), cerebrospinal fluid (33.7%/34.6%), middle ear fluid (60.2%/27.5%), and pleural fluid (50.0%/31.0%). Between 2003 and 2007, the frequency of penicillin resistance in invasive pneumococcal disease gradually decreased from 46.4% to 29.0% in children and from 43.8% to 32.7% in adults. This decrease coincided with the introduction of a seven-valent pneumococcal conjugate vaccine into immunization programs and with a general reduction in levels of antibiotic consumption in France

Polycomb repressive complex PRC1 spatially constrains the mouse embryonic stem cell genome.

The Polycomb repressive complexes PRC1 and PRC2 maintain embryonic stem cell (ESC) pluripotency by silencing lineage-specifying developmental regulator genes. Emerging evidence suggests that Polycomb complexes act through controlling spatial genome organization. We show that PRC1 functions as a master regulator of mouse ESC genome architecture by organizing genes in three-dimensional interaction networks. The strongest spatial network is composed of the four Hox gene clusters and early developmental transcription factor genes, the majority of which contact poised enhancers. Removal of Polycomb repression leads to disruption of promoter-promoter contacts in the Hox gene network. In contrast, promoter-enhancer contacts are maintained in the absence of Polycomb repression, with accompanying widespread acquisition of active chromatin signatures at network enhancers and pronounced transcriptional upregulation of network genes. Thus, PRC1 physically constrains developmental transcription factor genes and their enhancers in a silenced but poised spatial network. We propose that the selective release of genes from this spatial network underlies cell fate specification during early embryonic development

PLM Functionalities in the Fashion Industry. Preliminary Results of a Classification Framework

Part 10: Maturity Implementation and AdoptionInternational audienceAs widely known, Product Lifecycle Management (PLM) is a set of business solutions and tools for the management of the entire lifecycle of a product, from its conception to its disposal. Despite PLM was born in traditional contexts, recently it is increasingly used in other sectors such as the fashion industry, even if it shows several features that distances it from the traditional approach to PLM deployment. Request for customized products, rapid changes in customer’s preference and the consequent shorter product lifecycles make the fashion environment very complex. Within this scenario, PLM has the potential to enable fashion industry to reduce time to market and to increase competitiveness in the global scenario, but quite often clear guidelines are not available. Literature is not exhaustive, as we can’t find articles covering the whole set of functionalities. Quite often only few features have been mentioned and the same functionalities have been differently called. To overcome this gap, the purpose of this research is the definition of a framework on PLM functionalities in fashion, classified according to the macro-processes where they are involved. The result is an overview of the PLM functionalities organized in macro-processes, moving from the development of the creative idea to the arrival in the stores, validated by experts of PLM software houses working within the fashion industry. From a managerial point of view, it represents a clear guideline to support companies and vendors to identify the whole range of PLM functionalities and the processes positively involved during their implementation

Sensitivity analysis of the electrostatic force distance curve using Sobol’s method and design of experiments

International audienc