Double duplication in a nonrotational colon. Study of a case associated with mucinous adenoma

<p>Duplications of gastrointestinal tract are unusual anomalies, especially in the colon. We report a case with two colonic duplications in a 20 year old woman who complained of recurrent backache associated with left lower quadrant abdominal pain. Neither physical examination nor laboratory test alterations were detected. Roentgenographic examination showed organic scolinosis, no intestinal gas and a mass with central hyperdensity and thickened wall in the descending colon area on the CT scan. Laparotomy was performed in which 22 cm of ascending colon and terminal ileum sited on left side, was excised. Pathological study revealed two unrelated and cystic duplications without communication to intestinal lumen. Their epithelial lining were of colonic or gastric types and showed a mucinous adenoma; the remaining layers were normal except for eosinophilic inflammatory infiltrate with Charcot-Leyden like crystaloids. In conclusion, we present a double spherical colonic duplication type I associated to a mucinous adenoma on a non-rotational ascending colon in a patient with organic scoliosis.</p

Ultrastructural distribution of lectin-binding sites on gastric superficial mucus-secreting epithelial cells The role of Golgi apparatus in the initial glycosylation

<p>Normal human gastric epithelial cells were ex-amined by electron microscopy using each of five bi-otinylated lectins [Ulex europaeus agglutinin I (UEA-I), peanut agglutinin (PNA), wheat germ agglutinin (WGA), soybean agglutinin (SBA) and Dolichos biflorus agglutin-in (DBA)] as a probe. We employed 35 gastric surgical specimens removed from complicated peptic disease. The lectin-binding sites were revealed with streptavidin-col-loidal gold complex. All specimens were embedded in Spurr and LR White resins. In superficial foveolar epithe-lial cells, the lectins used were generally positive in all cell types (mainly UEA-1 and PNA) on the Golgi region and mucus cytoplasmic vacuoles, with many variations among cells in the same case. On the other hand, extracel-lular mucus was negative for WGA. Labelling with PNA revealed a biphasic pattern (peripheral positivity) on mu-cous droplets in surface and fove01ar cells. The cis side of the Golgi apparatus was labelled with SBA and PNA and rough endoplasmic reticulum with SBA (only five cases). Lectin-binding variability could be related to het-erogeneous composition of gastric mucus. Our results with SBA suggest initiation of O-glycosylation at the Golgi apparatus; however a role of the rough endoplas-mic reticulum cannot be excluded (N-glycosylation). We propose the following sequence of sugar addition to the carbohydrate side-chains of gastric glycoproteins: (1) GaNAc (Golgi apparatus cis-side), (2) GlcNAc (Golgi ap-paratus intermediate face), (3) GalNac or Gal, C~-L-fucose (Golgi apparatus trans-side).</p

Mixed cloacogenic carcinoma of male urethra

<p>Diagnostic features of poorly differentiated carcinoma with cloacogenic features of the male urethra.</p

Cutaneous ganglion cell choristoma. Report of a case

<p>We describe the histological, immunohistochemical, and ultrastructural findings of a cutaneous tumour composed of ganglion cells, without any other proliferating component. As ganglion cells are not normal components of the skin, we propose the term “ganglion cell choristoma” for this lesion. The differential diagnosis of related lesions such as cutaneous ganglioneuroma, well-differentiated metastases from neuroblastoma, autonomic ganglia entrapped by neuroflbroma, and reactive processes, and the possible histogenesis of ganglion cell choristoma are discussed.</p