Communication Strategy: Does the Two-Step Still Work?

Much of our work in cooperative extension deals with the transfer of information or technology to clientele. Therefore, it is extremely important that we use the most effective and efficient means possible in carrying out this task

Genomic evidence of functional diversity in DPANN archaea, from oxic species to anoxic vampiristic consortia

DPANN archaea account for half of the archaeal diversity of the biosphere, but with few cultivated representatives, their metabolic potential and environmental functions are poorly understood. The extreme geochemical and environmental conditions in meromictic ice-capped Lake A, in the Canadian High Arctic, provided an isolated, stratified model ecosystem to resolve the distribution and metabolism of uncultured aquatic DPANN archaea living across extreme redox and salinity gradients, from freshwater oxygenated conditions, to saline, anoxic, sulfidic waters. We recovered 28 metagenome-assembled genomes (MAGs) of DPANN archaea that provided genetic insights into their ecological function. Thiosulfate oxidation potential was detected in aerobic Woesearchaeota, whereas diverse metabolic functions were identified in anaerobic DPANN archaea, including degradation and fermentation of cellular compounds, and sulfide and polysulfide reduction. We also found evidence for “vampiristic” metabolism in several MAGs, with genes coding for pore-forming toxins, peptidoglycan degradation, and RNA scavenging. The vampiristic MAGs co-occurred with other DPANNs having complementary metabolic capacities, leading to the possibility that DPANN form interspecific consortia that recycle microbial carbon, nutrients and complex molecules through a DPANN archaeal shunt, adding hidden novel complexity to anaerobic microbial food webs

Reinterpreting aircraft measurements in anisotropic scaling turbulence

Due to both systematic and turbulent induced vertical fluctuations, the interpretation of atmospheric aircraft measurements requires a theory of turbulence. Until now virtually all the relevant theories have been isotropic or "quasi isotropic" in the sense that their exponents are the same in all directions. However almost all the available data on the vertical structure shows that it is scaling but with exponents different from the horizontal: the turbulence is scaling but anisotropic. In this paper, we show how such turbulence can lead to spurious breaks in the scaling and to the spurious appearance of the vertical scaling exponent at large horizontal lags. <br></br> We demonstrate this using 16 legs of Gulfstream 4 aircraft near the top of the troposphere following isobars each between 500 and 3200 km in length. First we show that over wide ranges of scale, the horizontal spectra of the aircraft altitude are nearly <i>k</i><sup>-5/3</sup>. In addition, we show that the altitude and pressure fluctuations along these fractal trajectories have a high degree of coherence with the measured wind (especially with its longitudinal component). There is also a strong phase relation between the altitude, pressure and wind fluctuations; for scales less than ≈40 km (on average) the wind fluctuations lead the pressure and altitude, whereas for larger scales, the pressure fluctuations leads the wind. At the same transition scale, there is a break in the wind spectrum which we argue is caused by the aircraft starting to accurately follow isobars at the larger scales. In comparison, the temperature and humidity have low coherencies and phases and there are no apparent scale breaks, reinforcing the hypothesis that it is the aircraft trajectory that is causally linked to the scale breaks in the wind measurements. <br></br> Using spectra and structure functions for the wind, we then estimate their exponents (β, <i>H</i>) at small (5/3, 1/3) and large scales (2.4, 0.73). The latter being very close to those estimated by drop sondes (2.4, 0.75) in the vertical direction. In addition, for each leg we estimate the energy flux, the sphero-scale and the critical transition scale. The latter varies quite widely from scales of kilometers to greater than several hundred kilometers. The overall conclusion is that up to the critical scale, the aircraft follows a fractal trajectory which may increase the intermittency of the measurements, but doesn't strongly affect the scaling exponents whereas for scales larger than the critical scale, the aircraft follows isobars whose exponents are different from those along isoheights (and equal to the vertical exponent perpendicular to the isoheights). We bolster this interpretation by considering the absolute slopes (|Δ<i>z</i>/Δ<i>x</i>|) of the aircraft as a function of lag Δ<i>x</i> and of scale invariant lag Δ<i>x</i>/Δ<i>z<sup>1/H<sub>z</sub></sup></i>. <br></br> We then revisit four earlier aircraft campaigns including GASP and MOZAIC showing that they all have nearly identical transitions and can thus be easily explained by the proposed combination of altitude/wind in an anisotropic but scaling turbulence. Finally, we argue that this reinterpretation in terms of wide range anisotropic scaling is compatible with atmospheric phenomenology including convection

A Study of the Problems of Cost Analysis in the Petroleum Industry

The significance of the subject of the present study-the cost of finding, developing and producing petroleum-can be better understood by placing it against a summary view of the supply situation in the postwar world. The most striking fact is the appearance of vast new reserves of oil in various parts of the world. Earlier concern over conservation was aroused by the fear of depletion of the available supply. Taking a long view, this possibility is not to be dismissed. But in recent years the practical problems of the industry, and of public policy toward it, are of a different sort. The capacity of the industry to produce in the countries of the free world greatly exceeds the current rates of consumption. From the sellers\u27 point of view, at least, it seems self-evident that too much oil exists today

Gradient-limited surfaces

A simple scenario of the formation of geological landscapes is suggested and the respective lattice model is derived. Numerical analysis shows that the arising non-Gaussian surfaces are characterized by the scale-dependent Hurst exponent, which varies from 0.7 to 1, in agreement with experimental data.Comment: 4 pages, 5 figure

Size-fractionated microbiome structure in subarctic rivers and a coastal plume across DOC and salinity gradients

Little is known about the microbial diversity of rivers that flow across the changing subarctic landscape. Using amplicon sequencing (rRNA and rRNA genes) combined with HPLC pigment analysis and physicochemical measurements, we investigated the diversity of two size fractions of planktonic Bacteria, Archaea and microbial eukaryotes along environmental gradients in the Great Whale River (GWR), Canada. This large subarctic river drains an extensive watershed that includes areas of thawing permafrost, and discharges into southeastern Hudson Bay as an extensive plume that gradually mixes with the coastal marine waters. The microbial communities differed by size-fraction (separated with a 3-μm filter), and clustered into three distinct environmental groups: (1) the GWR sites throughout a 150-km sampling transect; (2) the GWR plume in Hudson Bay; and (3) small rivers that flow through degraded permafrost landscapes. There was a downstream increase in taxonomic richness along the GWR, suggesting that sub-catchment inputs influence microbial community structure in the absence of sharp environmental gradients. Microbial community structure shifted across the salinity gradient within the plume, with changes in taxonomic composition and diversity. Rivers flowing through degraded permafrost had distinct physicochemical and microbiome characteristics, with allochthonous dissolved organic carbon explaining part of the variation in community structure. Finally, our analyses of the core microbiome indicated that while a substantial part of all communities consisted of generalists, most taxa had a more limited environmental range and may therefore be sensitive to ongoing change