A polyocular framework for research on multifunctional farming and rural development

The paradox of multifunctionality is that, on the one hand, the specialized functionalities of agriculture only arise because of the functional differentiation of social systems and scientific disciplines and, on the other hand, multifunctionality can only enter as a way to mediate between conflicts, interests and fragmented knowledge when different functions and observations of functions combine. The aim of this paper is to contribute to a theoretical and methodological platform for multidisciplinary research on multifunctional farming. With the notions of polyocular cognition and polyocular communication we introduce a second order, interdisciplinary communication process that can meet the challenge of creating a shared view on multifunctional farming. Polyocular communication must be based on other rules than the rules of the involved disciplines. Whereas disciplinary communication is about providing consistent, efficient and precise knowledge in the context of a sharply delimited research world, polyocular communication is about extending a multidimensional space of understanding

Exploring the potential of engineering polygalacturonase‐inhibiting protein as an ecological, friendly, and nontoxic pest control agent

In plants, polygalacturonase-inhibiting proteins (PGIPs) play critical roles for resistance to fungal disease by inhibiting the pectin-depolymerizing activity of endopolygalacturonases (PGs), one type of enzyme secreted by pathogens that compromises plant cell walls and leaves the plant susceptible to disease. Here, the interactions between PGIPs from Phaseolus vulgaris (PvPGIP1 and PvPGIP2) and PGs from Aspergillus niger (AnPG2), Botrytis cinerea (BcPG1 and BcPG2), and Fusarium moniliforme (FmPG3) were reconstituted through a yeast two hybrid (Y2H) system to investigate the inhibition efficiency of various PvPGIP1 and 2 truncations and mutants. We found that tPvPGIP2_5-8, which contains LRR5 to LRR8 and is only one-third the size of the full length peptide, exhibits the same level of interactions with AnPG and BcPGs as the full length PvPGIP2 via Y2H. The inhibitory activities of tPvPGIP2_5-8 on the growth of A. niger and B. cinerea were then examined and confirmed on pectin agar. On pectin assays, application of both full length PvPGIP2 and tPvPGIP2_5-8 clearly slows down the growth of A. niger and B. cinerea. Investigation on the sequence-function relationships of PGIP utilizing a combination of site directed mutagenesis and a variety of peptide truncations suggests that LRR5 could have the most essential structural feature for the inhibitory activities, and may be a possible target for the future engineering of PGIP with enhanced activity. This study highlights the potential of plant-derived PGIPs as a candidate for future in planta evaluation as a pest control agent