132 research outputs found
Relationship between survival and yield related traits in Solanum pimpinellifolium under salt stress
Development of Molecular Markers Tightly Linked to Pvr4 Gene in Pepper Using Next-Generation Sequencing
It is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. Potyvirus resistance 4 (Pvr4) locus in pepper confers resistance to three pathotypes of potato virus Y and to pepper mottle virus. We describe the use of next-generation sequencing technology to generate molecular markers tightly linked to Pvr4. Initially, comparative genomics was carried out, and a syntenic region of tomato on
chromosome ten was used to generate PCR-based markers and map Pvr4. Subsequently, the genomic sequence of pepper was used, and more than 5000 single-nucleotide variants (SNVs) were identified within the interval. In addition, we identified nucleotide binding siteâleucine-rich repeat-type disease resistance genes within the interval. Several of these SNVs were converted to molecular markers desirable
for large-scale molecular breeding programmes
Estimação dos componentes da variĂąncia fenotĂpica em feijoeiro utilizando o mĂ©todo genealĂłgico
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