Activity of Nitrifying Bacteria (Ammonia Oxidizer and Nitrite Oxidizer) in Brackishwater Ponds (Tambak) in Bengkalis Island, Riau Province

A research study was carried out from April to July 1999, on brackishwater ponds in Bengkalis Island, Riau Province, Indonesia. Observations and samplings were taken from three stations. Six nitrifying bacteria were isolated from ammonium oxidizer and four from nitrite oxidizer. The nitrifying bacteria activity varied from 0,024 to 0,092 ppm/day for ammonia oxidizer, and from 0,032 to 0,052 ppm/day for nitrite oxidizer. These nitrifying bacteria can reduce the ammonia concentration; in the brackish water ponds. In the absence of nitrifying bacteria, the ammonia concentration was so toxic, it was killed the shrimp at 31 days after samplin

Antagonism Test of Probiotics Bacteria Isolated From Black Tiger Shrimp (Penaeus Monodon) Against Pathogens (Aeromonas Hydrophila, Pseudomonas SP and Vibrio Alginolyticus)

Probiotics is one effective strategy in an effort to cope with the disease by pathogenic bacteria. The study aims to determine the level of pathogenic bacteria were isolated from the candidate probiotic intestinal tiger shrimp against pathogenic bacteria A. hydrophila, Pseudomonas sp and V. alginolyticus. Expected candidate probiotics can be used to combat the disease in fish and shrimp farming. Antagonism using disc diffusion test. Four isolates of candidate probiotic bacteria more dominant round, milky white color and yellow, smooth or scalloped edge. Four candidate probiotic bacteria can inhibit pathogenic bacteria A. hydrophila, Pseudomonas sp and V. alginolyticus. The highest inhibition zone on the candidate probiotics P1 against A. hydrophila, Pseudomonas sp of 11.7 mm and 11.1 mm. The highest inhibition zone on V.alginolyticus the candidate probiotic bacteria P2 of 11.4 mm

The Bacteriocin Antimicrobial Test Activity of Probiotic Bacteria Isolated From Giant Prawns (Macrobrachium Rosenbergii)

This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH4)2SO4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the paper disc

Effectivity of Nipah (Nypa Fruticans) Extract on (Aedes Aegypti) Larvae in Brackish Water

The most commonly used method of vektor control is temephos (the synthetic larvicide). Currently in some areas there has been resistance of aedes aegypti larvae to temephos, so that natural larvicide is required as an alternative. Nypa exctract contains flavonoid compounds that are respiratory toxins that can kill aedes aegypty larvae.This research was conducted in January - February 2019. The purpose of this study was to determine the inhibitory power of nipah extract against larvae in brackhish water. The results of this study are expected to increase knowledge and provide information about the pontential of nipah as a soursce of bioactive compounds. The method used is the experimental method, where the location of the research sampel was obtained at one sampling location point in the Sungai Kayu Ara village, Siak Riau province. The results showed that the average mortality of larvae in nipah laeves in the 60th minute was 55, 80, 100 and 100%. While root extracts have larvae deaths as many as 60 minutes, namely 100, 90, 90, and 100%. For the average mortality of larvae at the root of nipah there are 60 minutes, which are 95, 100, 100 and 100%. As wel as positive and negative controls as a comparison. So it can be compared to 3 administration of nipah extract both leaves, roots and midrib so that the three conncentrations used are better than the use of midrib extract compared to leaf and root extract. It can be concluded that the more concentration used the higher the mortality of larvae is the concentration of 12%. For the concentration used the percentage of larvae mortality in the test media is as much as 6%, 9% and 12%. Every death of larvae has reristance to different bodies so that at the 30th minute there is a difference in mortality of larvae wich a given concentration. The larvae species used are aedes aegyti larvae. The highest mortality of dead es was found in midrib extract and also positive controls. So that the death of larvae is very effective against the given solution has the same ability. The mortality of A. aegypti larvae were then analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. The results of the probit analysis of leaf, root and midrib extracts are the estimation of LC50 1.493, 2.618, 0.005. while leaf , root and midrib extracts at the LT50 estimate were 2.841, 1.579, 2.22

Efektivitas Bakteri Probiotik Dari Udang Galah (Macrobrachium Rosenbergii, De Man) Terhadap Bakteri Patogen Aeromonas Hydrophila, Pseudomonas Stutzeri Dan Vibrio Alginolyticus

Probiotic bacteria capable of producing substances like antibiotic sandin dispensable for being able to suppressthe growth of pathogenic bacteria into disease and parasites in aquaculture. The research purposeof this study to find probiotic isolates were able to controlthe disease in aquaculture. The candidate probiotic bacteria isolated from prawn (Macrobrachium rosenbergii, de Man) by the method of scratch on TSA and NA media. Anti-bacterial activity using media discs on Zobell and TSA. 4 isolates were found which consisted of coccus, white and orange colonies. All isolates had inhibitory effect onthe growth of pathogenic bacteria. The highest sensitivitytest is indicated by P4 DUG isolates of A.hydrophilla at13,30, 13,20 and14,10 of P.stutzeri in V.alginolyticu

Isolation and Antagonism of Bacteria Test of Biota in the Mangrove Ecosystem Kayu Ara River Siak Regency

The General area is the mangrove biodiversity and marine life that have biological activity that likely has the same pharmacological potential as an antimicrobial compound. The purpose of this research is to know the bacterial antagonism in isolation from mangrove, mangrove crabs, snails and fish tembakul against pathogenic bacteria (Aeromonas hydrophila, Aeromonas salmonicida, Edwardsiella ictaluri). This research dimula from May – June 2019. From the results of research that has been done shows that isolates that have the capability of antagonism against pathogenic bacteria test three (A. salmonicida, A. hydrophila and E. ictaluri) were isolate CS7 with category stronger, isolate CS6, CS5, CS4, AI4, AI5, CS9 AI2, with weak category, whereas isolates CS1, B22, B33, B44, AI6 AI7 AI8, was only able to inhibit pathogenic test of 2 with a weak category. The results of the DNA analysis of the 16S rRNA sequence with the use and analysis of BLAST obtained that isolates the Bacillus cereus species are included

Using of Bacteria Heterotrophic as an Anti-Bacterial Againts Pathogenic Bacteria Isolated From Sea Water in Dumai City , Riau Province

Heterotrophic bacteria have a role as decomposer of organic compounds (mineralization) and inorganic nitrogen (ammonia, nitrite, and nitrate) derived from industrial waste, decomposition of uneaten feed, faecal, excretion of fish and have the ability to inhibit the growth of pathogenic bacteria. This research was conducted in November untill December 2016. The results of antagonism test showed isolated bacteria have the ability to inhibit the growth of pathogenic bacteria (Vibrio algynolyticus, Aeromonas hydrophila and Pseudomonas sp.) Zone of inhibition in D5, D6 and D4 isolates classified in the strong category with zone of inhibition average of 10.5 to 11.8 mm and zone of inhibition in D1, D2, D3, D5, D7 isolates classified in the medium category with zone of inhibition average of 5.3 to 8.5 mm and each of the isolates are also classified in the weak category. The results of DNA analysis of heterotrophic bacteria isolated from marine waters of industrial area and low salinity of estuarine waters by using the PCR method technique 16S sequences found three strains of bacteria from the highest level of homology Bacillus toyonensis, Bacillus cereus and Bacillus pseudomycoides. D1 isolates has similarity to Bacillus cereus strain ASK16, D2 isolates to Bacillus toyonensis strain AC OPR1150Xg, D3 isolates to Bacillus pseudomycoides strain F1-8, D4 isolates to Bacillus cereus strain SBFW51, D5 isolates to Bacillus cereus strain OPP5 3-2, D6 to Bacillus cereus strain SBFW5S, D7 isolates to Bacillus cereus strain B4. Most of isolated bacteria obtained from the waters of industrial area because it receives much of nutrients that are very influential in the growth of bacteria

Test of Bacteriocin Antimicrobial Activity of Probiotic Bacteria Isolated From Tiger Shrimp (Penaeus Monodon Fabricus)

Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR) without precipitation was 365,65mm2/ml at UWH10 isolate toward bacteria test P. stutzeri

Antagonis Bakteri Probiotik yang Diisolasi dari Usus dan Lambung Ikan Kerapu Bebek (Cromileptes Altivelis) terhadap Bakteri Patogen

Grouper fish has a fairly high economic value, especially in Asiancountries, namely Hong Kong, Singapore, Taiwan and China. High marketdemand. On aquaculture, is one important component of fish feed that plays animportant role in the cultivation. Application of probiotics in aquaculture fish feedhas not been done yet in the Fisheries sector. The research was conducted inOctober 2008, Grouper is used as the intestines and stomach samples were takenas a source of probiotics, whereas pathogenic bacteria obtained from thecollection of marine microbiology laboratory of Faculty of Fisheries and MarineRiau University. From the isolation of bacterial strains found 11 strains that havethe potential as probiotics, then proceed to the molecular test by using rDNA16 s.Which is obtained two strains of probiotic bacteria Bacillus cereus and the speciesof bacteria Strenotrophomonas maltophilia. The test results found that theantagonist Bacillus cereus isolates to inhibit the growth of pathogenic bacteriaAeromonas sp and two species of Vibrio sp. While the bacteriaStrenotrophomonas maltophilia showed no antagonism against pathogens