Stochastic Ratchet Mechanisms for Replacement of Proteins Bound to DNA

Experiments indicate that unbinding rates of proteins from DNA can depend on the concentration of proteins in nearby solution. Here we present a theory of multi-step replacement of DNA-bound proteins by solution-phase proteins. For four different kinetic scenarios we calculate the depen- dence of protein unbinding and replacement rates on solution protein concentration. We find (1) strong effects of progressive 'rezipping' of the solution-phase protein onto DNA sites liberated by 'unzipping' of the originally bound protein; (2) that a model in which solution-phase proteins bind non-specifically to DNA can describe experiments on exchanges between the non specific DNA- binding proteins Fis-Fis and Fis-HU; (3) that a binding specific model describes experiments on the exchange of CueR proteins on specific binding sites.Comment: \`a paraitre en PHys. Rev. Lett. june 201

Slow nucleic acid unzipping kinetics from sequence-defined barriers

Recent experiments on unzipping of RNA helix-loop structures by force have shown that about 40-base molecules can undergo kinetic transitions between two well-defined `open' and `closed' states, on a timescale = 1 sec [Liphardt et al., Science 297, 733-737 (2001)]. Using a simple dynamical model, we show that these phenomena result from the slow kinetics of crossing large free energy barriers which separate the open and closed conformations. The dependence of barriers on sequence along the helix, and on the size of the loop(s) is analyzed. Some DNAs and RNAs sequences that could show dynamics on different time scales, or three(or more)-state unzipping, are proposed.Comment: 8 pages Revtex, including 4 figure

How Do House Prices Affect Consumption? Evidence From Micro Data

Housing is a major component of wealth. Since house prices fluctuate considerably over time, it is important to understand how these fluctuations affect households' consumption decisions. Rising house prices may stimulate consumption by increasing households' perceived wealth, or by relaxing borrowing constraints. This paper investigates the response of household consumption to house prices using UK micro data. We estimate the largest effect of house prices on consumption for older homeowners, and the smallest effect, insignificantly different from zero, for younger renters. This finding is consistent with heterogeneity in the wealth effect across these groups. In addition, we find that regional house prices affect regional consumption growth. Predictable changes in house prices are correlated with predictable changes in consumption, particularly for households that are more likely to be borrowing constrained, but this effect is driven by national rather than regional house prices and is important for renters as well as homeowners, suggesting that UK house prices are correlated with aggregate financial market conditions.

Household Risk Management and Optimal Mortgage Choice

Home mortgages are the most significant financial contract for many households. The form of this contract is correspondingly important. This paper studies the choice between fixed-rate (FRM) and adjustable-rate (ARM) mortgages. In an environment with uncertain inflation, nominal FRMs have risky real capital value whereas ARMs have safe capital value. However ARMs can greatly increase the short-term variability of required real interest payments. This is a serious disadvantage of ARMs for households who face borrowing constraints and have only a small buffer stock of financial assets. The paper uses numerical methods to solve a life-cycle model with risky labor income and borrowing constraints, under alternative assumptions about available mortgage contracts. Households with large mortgages, risky labor income, high risk aversion, and a low probability of moving are more likely to prefer nominal FRMs. The paper also considers inflation-indexed FRMs. These mortgages remove the wealth risk of nominal FRMs without incurring the income risk of ARMs, and therefore are a superior vehicle for household risk management. The paper finds that the welfare gains of mortgage indexation can be very large.

Household Risk Management and Optimal Mortgage Choice

A typical household has a home mortgage as its most significant financial contract. The form of this contract is correspondingly important. This paper studies the choice between a fixed-rate (FRM) and an adjustable-rate (ARM) mortgage. In an environment with uncertain inflation, a nominal FRM has risky real capital value whereas an ARM has a stable real capital value. However an ARM can increase the short-term variability of required real interest payments. This is a disadvantage of the ARM for a household that faces borrowing constraints and has only a small buffer stock of financial assets. The paper uses numerical methods to solve a life-cycle model with risky labor income and borrowing constraints, under alternative assumptions about available mortgage contracts. While an ARM is generally an attractive form of mortgage, a household with a large mortgage, risky labor income, high risk aversion, a high cost of default, and a low probability of moving is less likely to prefer an ARM. The paper also considers an inflation-indexed FRM, which removes the wealth risk of the nominal FRM without incurring the income risk of the ARM, and is therefore a superior vehicle for household risk management. The welfare gain from mortgage indexation can be very large.

How Do House Prices Affect Consumption? Evidence From Micro F. Data

Housing is a major component of wealth. Since house prices fluctuate considerably over time, it is important to understand how these fluctuations affect households’ consumption decisions. Rising house prices may stimulate consumption by increasing households’ perceived wealth, or by relaxing borrowing constraints. This paper investigates the response of household consumption to house prices using UK micro data. We estimate the largest effect of house prices on consumption for older homeowners, and the smallest effect, insignificantly different from zero, for younger renters. This finding is consistent with heterogeneity in the wealth effect across these groups. It suggests that as the population ages and becomes more concentrated in the old homeowners group, aggregate consumption may become more responsive to house prices. In addition, we find that regional house prices affect regional consumption growth. Predictable changes in house prices are correlated with predictable changes in consumption, particularly for households that are more likely to be borrowing constrained, but this effect is driven by national rather than regional house prices and is important for renters as well as homeowners, suggesting that UK house prices are correlated with aggregate financial market conditions.

How to enhance crop production and nitrogen fluxes? A result-oriented scheme to evaluate best agri-environmental measures in Veneto Region, Italy

The cost-effectiveness of adopting agri-environmental measures (AEMs) in Europe, which combine agricultural productions with reduced N losses, is debated due to poorly targeted site-specific funding that is allocated regardless of local variability. An integrated DAYCENT model-GIS platform was developed combining pedo-climatic and agricultural systems information. The aim was to evaluate best strategies to improve N fluxes of agro-ecosystems within a perspective of sustainable intensification. Indicators of agronomic efficiency and environmental quality were considered. The results showed that agronomic benefits were observed with a continuous soil cover (conservation agriculture and cover crops), which enhanced nitrogen use efficiency (+17%) and crop yields (+34%), although in some cases these might be overestimated due to modelling limitations. An overall environmental improvement was found with continuous soil cover and long-term change from mineral to organic inputs (NLeach 45 Mg ha 121), which were effective in the sandy soils of western and eastern Veneto with low SOM, improving the soil-water balance and nutrients availability over time. Results suggest that AEM subsidies should be allocated at a site-specific level that includes pedo-climatic variability, following a result-oriented approach

VGF changes during the estrous cycle: a novel endocrine role for TLQP peptides?

Although the VGF derived peptide TLQP-21 stimulates gonadotropin-releasing hormone (GnRH) and gonadotropin secretion, available data on VGF peptides and reproduction are limited. We used antibodies specific for the two ends of the VGF precursor, and for two VGF derived peptides namely TLQP and PGH, to be used in immunohistochemistry and enzyme-linked immunosorbent assay complemented with gel chromatography. In cycling female rats, VGF C-/N-terminus and PGH peptide antibodies selectively labelled neurones containing either GnRH, or kisspeptin (VGF N-terminus only), pituitary gonadotrophs and lactotrophs, or oocytes (PGH peptides only). Conversely, TLQP peptides were restricted to somatostatin neurones, gonadotrophs, and ovarian granulosa, interstitial and theca cells. TLQP levels were highest, especially in plasma and ovary, with several molecular forms shown in chromatography including one compatible with TLQP-21. Among the cycle phases, TLQP levels were higher during metestrus-diestrus in median eminence and pituitary, while increased in the ovary and decreased in plasma during proestrus. VGF N- and C-terminus peptides also showed modulations over the estrous cycle, in median eminence, pituitary and plasma, while PGH peptides did not. In ovariectomised rats, plasmatic TLQP peptide levels showed distinct reduction suggestive of a major origin from the ovary, while the estrogen-progesterone treatment modulated VGF C-terminus and TLQP peptides in the hypothalamus-pituitary complex. In in vitro hypothalamus, TLQP-21 stimulated release of growth hormone releasing hormone but not of somatostatin. In conclusion, various VGF peptides may regulate the hypothalamus-pituitary complex via specific neuroendocrine mechanisms while TLQP peptides may act at further, multiple levels via endocrine mechanisms involving the ovary

Mechanical influence of inherited folds in thrust development: A case study from the variscan fold-and-thrust belt in SW sardinia (Italy)

Fold-and-thrust belts have a high variability of structural styles, whose investigation provides continuous updates of the predictive models that try to better approximate the geometries recognized in the field. The majority of studies are focused on the geometry and development of folds and thrust surfaces and the amount of displacement, taking into account the role played by the involved stratigraphic succession assumed as a layer cake. We present a case study from the external zone of the Variscan fold-and-thrust belt in SW Sardinia, where it was possible to investigate the lateral and vertical variations of the mechanical properties of the involved succession, how they related to previous folding, control thrust geometry, and kinematics. In this case, the superposition of two fold systems acted as a buttress that induced extensive back-thrusting. We found that there is a close connection between the attitude of the bedding and the geometry of back thrust surfaces, shear strength during thrust propagation, and variation in the shortening amount, de-pending on which part of the folds were cut across. The folding-related mechanical anisotropy also seems to have induced a ductile deformation in the footwall of back-thrusts. Although the case study considers the development of back-thrust, the relations between thrust and not-layer cake geometries could also be applied to fore-thrust development

Monitoring pit and fissures using transparent sealant and fluorescence intraoral camera, 12 months follow up

The aim of this in vivo study is to report on the combined use of a fluorescence intraoral camera and transparent sealant for the clinical monitoring of pits and fissures. 96 permanent molars with a ICDAS II code 0, 1, or 2, (in 48 patients aged 12–14) were registered at the First Observation Unit (Oral and Maxillofacial Sciences Department), Sapienza University, Rome. Clinically selected teeth were double-checked using a VistaCam iX Proof (Durr Dental AG) and sealed with a transparent sealant (ControlSeal, VOCO GmbH), following the established indications for use if a pit and fissure condition was confirmed within the camera’s internal cutoff point of 1.5 (“early enamel demineralization”). Clinical followup was performed using VistaCam at 6 and 12 months to assess sealant retention and any demineralization trend. At baseline, 57.4% of the registered teeth were sound, both visually and when using the fluorescence camera, 42.6% presented an early demineralization (<1.5 with VistaCam and ICDAS II 1- 2). Subsequent VistaCam assessment of surfaces underlying the transparent sealant totally confirmed initial evaluations. Complete sealant retention rated 95% at 6 months, and 91% at 12 months. No case of complete detachment was observed. At the 12-month follow-up, VistaCam measurements resulted stable in the whole sample, except for one permanent molar, which presented a demineralization increment and partial sealant retention. Visual and fluorescence assessments were consistent and feasible. Incomplete sealant retention occurred in 5% of cases at 6 months and 9% of cases at 12 months and was probably due to procedure imperfections. The combined use of transparent sealant and a fluorescence camera shows clinical effectiveness and diagnostic efficacy for occlusal surface monitoring