Thermal model of successive dike injections and implications for the development of intraplate volcanoes

Temperatures in the root zones of volcanoes play a critical role in the development and persistence of shallow-level magmatic reservoirs in the crust. Here, we present a 1D thermal model allowing evaluation of the thermal impact of magma travelling in conduits to the surface on the root zone of a volcano. This thermal model has been developed to better understand the formation of a vertical intrusion located in the root zone of a dismembered Miocene volcano on Fuerteventura, Canary Archipelago. This intrusion, named PX1, constitutes an almost pure amalgamation of dikes of either clinopyroxenitic or gabbroic composition. Both types of dikes display cumulate textures and are interpreted as resulting from the protracted crystallization of a mafic magma. The formation of clinopyroxenitic, in contrast to gabbroic dikes, requires that the residual melt was extracted at high temperature (N1050°) to avoid plagioclase crystallization. Simulations of multiple dike injections show that the temperature in the root zone increases significantly with the addition of dikes, but the maximum temperature reached in the system depends on the duration of magma flow in the conduits and the time interval between dike injections (i.e., repose period). Active flow is the critical parameter that distinguishes instantaneous dike injection from a magmatic conduit. Without significant magma flow (N1 month), high-temperature conditions (N1000 °C) cannot be maintained in the pluton unless dikes are very thick and the repose period is extremely small. On the other hand,magma flow times of one to several months, combined with short time intervals between dike injections (b25 years), which are conditions comparable to those recorded for historical eruptions of oceanic island volcanoes, allowthe production and preservation of temperatures above the plagioclase liquidus for significant durations, as required to generate clinopyroxenitic dikes such as those observed in the PX1 pluton. Persistent high temperature in the vicinity of magma conduits limits the differentiation of melts in transit to the surface, providing a potential explanation forwhy lavas ofmafic to intermediate composition predominate in intraplate volcanoes such as Fuerteventura or Fogo Island (Cape Verde Archipelago). In extreme cases, when temperatures over 1000–1050 °C in the central part of the feeding zone are maintained for years, the remaining magma in the conduit does not solidify but is preserved in a mushy state. New pulses of magma would not be able to cross this zone butwould rather amalgamate in the incipientmagma reservoir. The present model differs from previous models of sill intrusion in that magmas do not need to pond at depth to create a reservoir but merely supply heat while travelling to the surface. Depending on the time interval between dike injections and the duration ofmagma flow through the crust, magma rising in vertical conduits could directly feed the volcanic edifice or could lead to the formation of magma reservoirs. This process may explain why some volcanoes erupt mafic or differentiated magmas during distinct periods of activity

The Expanded Kinesin-13 Repertoire of Trypanosomes Contains Only One Mitotic Kinesin Indicating Multiple Extra-Nuclear Roles

BACKGROUND: Kinesin-13 proteins have a critical role in animal cell mitosis, during which they regulate spindle microtubule dynamics through their depolymerisation activity. Much of what is known about Kinesin-13 function emanates from a relatively small sub-family of proteins containing MCAK and Kif2A/B. However, recent work on kinesins from the much more widely distributed, ancestral Kinesin-13 family, which includes human Kif24, have identified a second function in flagellum length regulation that may exist either alongside or instead of the mitotic role. METHODOLOGY/PRINCIPAL FINDINGS: The African trypanosome Trypanosoma brucei encodes 7 distinct Kinesin-13 proteins, allowing scope for extensive specialisation of roles. Here, we show that of all the trypanosomal Kinesin-13 proteins, only one is nuclear. This protein, TbKIN13-1, is present in the nucleoplasm throughout the cell cycle, but associates with the spindle during mitosis, which in trypanosomes is closed. TbKIN13-1 is necessary for the segregation of both large and mini-chromosomes in this organism and reduction in TbKIN13-1 levels mediated by RNA interference causes deflects in spindle disassembly with spindle-like structures persisting in non-mitotic cells. A second Kinesin-13 is localised to the flagellum tip, but the majority of the Kinesin-13 family members are in neither of these cellular locations. CONCLUSIONS/SIGNIFICANCE: These data show that the expanded Kinesin-13 repertoire of trypanosomes is not associated with diversification of spindle-associated roles. TbKIN13-1 is required for correct spindle function, but the extra-nuclear localisation of the remaining paralogues suggests that the biological roles of the Kinesin-13 family is wider than previously thought

Functional Characterisation and Drug Target Validation of a Mitotic Kinesin-13 in Trypanosoma brucei

Mitotic kinesins are essential for faithful chromosome segregation and cell proliferation. Therefore, in humans, kinesin motor proteins have been identified as anti-cancer drug targets and small molecule inhibitors are now tested in clinical studies. Phylogenetic analyses have assigned five of the approximately fifty kinesin motor proteins coded by Trypanosoma brucei genome to the Kinesin-13 family. Kinesins of this family have unusual biochemical properties because they do not transport cargo along microtubules but are able to depolymerise microtubules at their ends, therefore contributing to the regulation of microtubule length. In other eukaryotic genomes sequenced to date, only between one and three Kinesin-13s are present. We have used immunolocalisation, RNAi-mediated protein depletion, biochemical in vitro assays and a mouse model of infection to study the single mitotic Kinesin-13 in T. brucei. Subcellular localisation of all five T. brucei Kinesin-13s revealed distinct distributions, indicating that the expansion of this kinesin family in kinetoplastids is accompanied by functional diversification. Only a single kinesin (TbKif13-1) has a nuclear localisation. Using active, recombinant TbKif13-1 in in vitro assays we experimentally confirm the depolymerising properties of this kinesin. We analyse the biological function of TbKif13-1 by RNAi-mediated protein depletion and show its central role in regulating spindle assembly during mitosis. Absence of the protein leads to abnormally long and bent mitotic spindles, causing chromosome mis-segregation and cell death. RNAi-depletion in a mouse model of infection completely prevents infection with the parasite. Given its essential role in mitosis, proliferation and survival of the parasite and the availability of a simple in vitro activity assay, TbKif13-1 has been identified as an excellent potential drug target

Gene Discovery in the Threatened Elkhorn Coral: 454 Sequencing of the Acropora palmata Transcriptome

BACKGROUND: Cnidarians, including corals and anemones, offer unique insights into metazoan evolution because they harbor genetic similarities with vertebrates beyond that found in model invertebrates and retain genes known only from non-metazoans. Cataloging genes expressed in Acropora palmata, a foundation-species of reefs in the Caribbean and western Atlantic, will advance our understanding of the genetic basis of ecologically important traits in corals and comes at a time when sequencing efforts in other cnidarians allow for multi-species comparisons. RESULTS: A cDNA library from a sample enriched for symbiont free larval tissue was sequenced on the 454 GS-FLX platform. Over 960,000 reads were obtained and assembled into 42,630 contigs. Annotation data was acquired for 57% of the assembled sequences. Analysis of the assembled sequences indicated that 83-100% of all A. palmata transcripts were tagged, and provided a rough estimate of the total number genes expressed in our samples (~18,000-20,000). The coral annotation data contained many of the same molecular components as in the Bilateria, particularly in pathways associated with oxidative stress and DNA damage repair, and provided evidence that homologs of p53, a key player in DNA repair pathways, has experienced selection along the branch separating Cnidaria and Bilateria. Transcriptome wide screens of paralog groups and transition/transversion ratios highlighted genes including: green fluorescent proteins, carbonic anhydrase, and oxidative stress proteins; and functional groups involved in protein and nucleic acid metabolism, and the formation of structural molecules. These results provide a starting point for study of adaptive evolution in corals. CONCLUSIONS: Currently available transcriptome data now make comparative studies of the mechanisms underlying coral's evolutionary success possible. Here we identified candidate genes that enable corals to maintain genomic integrity despite considerable exposure to genotoxic stress over long life spans, and showed conservation of important physiological pathways between corals and bilaterians