11 research outputs found
Data_Sheet_1_Altered Expression of CD300a Inhibitory Receptor on CD4+ T Cells From Human Immunodeficiency Virus-1-Infected Patients: Association With Disease Progression Markers.PDF
<p>The ability of the CD300a inhibitory receptor to modulate immune cell functions and its involvement in the pathogenesis of many diseases has aroused a great interest in this molecule. Within human CD4+ T lymphocytes from healthy donors, the inhibitory receptor CD300a is differentially expressed among different T helper subsets. However, there are no data about the expression and regulation of CD300a receptor on CD4+ T cells from human immunodeficiency virus (HIV)-1-infected patients. The objective of this study was to investigate the expression of CD300a on CD4+ T cells from HIV-infected patients on suppressive combined antiretroviral therapy (cART) and cART naĂŻve patients. Our results have demonstrated that the expression levels of this inhibitory receptor were higher on CD4+ T cells from HIV-1 infected subjects compared with healthy donors, and that cART did not reverse the altered expression of CD300a receptor in these patients. We have observed an increase of CD300a expression on both PD1+CD4+ and CD38+CD4+ T cells from HIV-1 infected people. Interestingly, a triple positive (CD300a+PD1+CD38+) subset was expanded in naĂŻve HIV-1 infected patients, while it was very rare in healthy donors and patients on cART. Finally, we found a negative correlation of CD300a expression on CD4+ T lymphocytes and some markers associated with HIV-1 disease progression. Thus, our results show that HIV-1 infection has an impact in the regulation of CD300a inhibitory receptor expression levels, and further studies will shed light into the role of this cell surface receptor in the pathogenesis of HIV infection.</p
Additional file 7: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Immunological variables (in CD4 and CD8 T-cell subsets) with significant differences between EC cases and EC controls (Mann–Whitney U test). (DOC 52 kb
Additional file 2: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Monoclonal antibodies and fluorochromes used in the study. (DOC 49 kb
Additional file 4: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Partial least-squares–class modeling (PLS-CM). (DOC 33 kb
Additional file 1: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Flow diagram showing the inclusion criteria and the sequential strategy for selecting the patients included in the study. Numbers inside the boxes indicate the number of patients selected after each step of the selection process. (DOC 200 kb
Additional file 5: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Immunological variables (in CD4 and CD8 T-cell subsets) with significant differences between EC patients and healthy subjects (HCs) (Mann–Whitney U test). (DOC 119 kb
Additional file 3: of Class-modeling analysis reveals T-cell homeostasis disturbances involved in loss of immune control in elite controllers
Staining conditions for immunophenotypic analysis and definitions of T-cell subsets analyzed. (DOC 29 kb
Immunologic evolution during the last 5 years of HIV control according to the event ending virological control (in 794 HIV controllers with both CD4 and CD8 measurements during this period).
<p>Immunologic evolution during the last 5 years of HIV control according to the event ending virological control (in 794 HIV controllers with both CD4 and CD8 measurements during this period).</p
Cumulative incidence functions for loss of virological control, ART initiation and death since confirmation of HIV controller status.
<p>Cumulative incidence functions for loss of virological control, ART initiation and death since confirmation of HIV controller status.</p
Characteristics according to the outcome of virological control in 1067 HIV controllers from COHERE Collaboration.
<p>Characteristics according to the outcome of virological control in 1067 HIV controllers from COHERE Collaboration.</p