Oil and Gas Map of Warren County, KY

Oil and gas map of Warren County, Kentucky / prepared under the direction of W.R. Jillson by W.C. Eyl

Reproducible, Ultra High-Throughput Formation of Multicellular Organization from Single Cell Suspension-Derived Human Embryonic Stem Cell Aggregates

Background: Human embryonic stem cells (hESC) should enable novel insights into early human development and provide a renewable source of cells for regenerative medicine. However, because the three-dimensional hESC aggregates [embryoid bodies (hEB)] typically employed to reveal hESC developmental potential are heterogeneous and exhibit disorganized differentiation, progress in hESC technology development has been hindered. Methodology/Principal Findings: Using a centrifugal forced-aggregation strategy in combination with a novel centrifugalextraction approach as a foundation, we demonstrated that hESC input composition and inductive environment could be manipulated to form large numbers of well-defined aggregates exhibiting multi-lineage differentiation and substantially improved self-organization from single-cell suspensions. These aggregates exhibited coordinated bi-domain structures including contiguous regions of extraembryonic endoderm- and epiblast-like tissue. A silicon wafer-based microfabrication technology was used to generate surfaces that permit the production of hundreds to thousands of hEB per cm 2. Conclusions/Significance: The mechanisms of early human embryogenesis are poorly understood. We report an ultra high throughput (UHTP) approach for generating spatially and temporally synchronised hEB. Aggregates generated in this manner exhibited aspects of peri-implantation tissue-level morphogenesis. These results should advance fundamental studies into early human developmental processes, enable high-throughput screening strategies to identify conditions tha

Measurement of the neutron electric form factor GE,n in the quasifree 2H(e→,e′n→)p reaction

The electric form factor of the neutron G E , n has been measured in the quasifree 2 H ( → e , e ′ → n ) p reaction using the 855 MeV polarized cw electron beam of the Mainz Microtron MAMI. The polarization of the scattered neutrons was analyzed in a polarimeter consisting of two walls of plastic scintillators. The precession of the neutron spin in a magnetic field was used for the first time to circumvent the measurement of the effective analyzing power of the polarimeter and the beam polarization. In this way G E , n could be determined with little model dependence and experimental uncertainties. The result G E , n ( 0.34 GeV 2 / c 2 ) = 0.0611 ± 0.0069 stat ( { + 0.0069 } { − 0.0055 } ) syst is larger than previously assumed

Determination of the neutron electric form factor in the D(e⃗,e′n⃗)pD(\vec{e},e^{\prime}\vec{n})p reaction and the influence of nuclear binding

The electric form factor of the neutron $G_{E,n}$ has been determined at the Mainz Microtron MAMI at the low momentum transfer $Q^{2}=0.15 \,{(GeV/c)}^{2}$ in a measurement of the recoil polarisation ratio $P_{x}/P_{z}$ in the quasifree reaction $D(\vec{e},e^{\prime}\vec{n})p$. At this $Q^{2}$ the influence of the nuclear binding is strong. A purely kinematical model is used to get some insight into the effect of the initial Fermi momentum distribution of the neutron. The influence of the final state interaction is determined quantitatively by a model of Arenhövel et al.. After the corresponding corrections a value of $G_{E,n}(0.15\,{(GeV/c)}^{2}) = 0.0481 \pm0.0065_{stat} \pm0.0053_{syst}$ is obtained

Determination of the neutron electric form factor in the D(e,e′n)p reaction and the influence of nuclear binding

The electric form factor of the neutron GE,n has been determined at the Mainz Microtron MAMI at the low momentum transfer Q2= 0.15 (GeV/c)2 in a measurement of the recoil polarisation ratio Px/Pz in the quasifree reaction D(e,e′n)p. At this Q2 the influence of the nuclear binding is strong. A purely kinematical model is used to get some insight into the effect of the initial Fermi momentum distribution of the neutron. The influence of the final state interaction is determined quantitatively by a model of Arenhövel et al.. After the corresponding corrections a value of GE,n(0.15 (GeV/c)2) = 0.0481±0.0065stat±0.0053syst is obtained