Fungal diseases of trees and shrubs growing in Siberian Square in Warsaw

A good condition of trees and shrubs growing in urban green areas is one of the key aspects of management. Plant monitoring, including recognition of symptoms of diseases caused by pathogenic fungi and prevention of their spread, is one of the ways of supporting the process of maintenance of green areas. The aim of the study was to conduct preliminary investigations of plants growing in Siberian Square in Warsaw infected by phytopathogenic fungi. The research carried out in 2017-2019 in Siberian Square in Warsaw revealed that the most common diseases observed on the trees and shrubs were caused by powdery mildew fungi infecting maples, poplars, apple trees, and barberry shrubs. Sawadaea tulasnei, Erysiphe adunca, Erysiphe berberidis, and Podosphaera leucotricha (Erysiphales) were identified as the causal agents of powdery mildew. Pathogens causing leaf spots and leaf lesions were identified as Venturia inaequalis on Malus x purpurea ’Ola’ and Rhytisma acerinum on Acer platanoides. Rust caused by Melampsora laricis-populina (Pucciniales) and basidiomata of Pleurotus ostreatus were detected on infected Populus nigra ‘Italica’ tree

Phylogenetic Relationships between Phlebiopsis gigantea and Selected Basidiomycota Species Inferred from Partial DNA Sequence of Elongation Factor 1-Alpha Gene

Phlebiopsis gigantea (Fr.) Jülich has been successfully used as a biological control fungus for Heterobasidion annosum (Fr.) Bref., an important pathogen of pine and spruce trees. The P. gigantea species has been known for many years, but our understanding of the relationship between various isolates of this fungus has been substantially improved through the application of DNA sequence comparisons. In this study, relationships between P. gigantea and selected Basidiomycota species was determined, based on elongation factor 1-alpha (EF1α) partial DNA sequence and in silico data. A total of 12 isolates, representing the most representatives of P. gigantea, with diverse geographic distributions and hosts, were included in this study. Phylogenetic trees generated for sequences obtained in this research, grouped the European taxa of P. gigantea and partial sequence of the genome deposed in NCBI database, in a strongly supported clade, basal to the rest of the strains included in the study. P. gigantea isolates originating from Poland, Finland, Sweden, Great Britain and partial sequence of genome formed a monophyletic group. Within this group, isolates of P. gigantea constituted two subclades, showing their partial difference like the two SNPs (single nucleotide polymorphisms) between one and the rest of isolates. The intron and exon relationships among P. gigantea isolates were moreover resolved. The results obtained using the EF1α region should be useful in the selection of more efficient P. gigantea isolates for limiting forest tree root pathogens

Influence of Endosperm Starch Composition on Maize Response to Fusarium temperatum Scaufl. & Munaut

Fusarium temperatum Scaufl. & Munaut is a newly described taxon belonging to the Fusarium fujikuroi species complex (FFSC) and a frequent causative factor of maize ear rot. The aim of the present study was to determine the responses to the disease in maize populations differing in endosperm features that were classified to flint, dent, and a group of plants with intermediate kernel characteristics. In inoculation studies, substantial variation of host response to the fungus was found among the tested maize types. The dent-type kernels contained significantly less amylose (28.27%) and exhibited significantly higher rates of infection (IFER = 2.10) and contamination by beauvericin (7.40 mg kg−1) than plants of the flint maize subpopulation. The study documents a significant positive correlation between the Fusarium ear rot intensity (IFER) and ergosterol content (the R value ranged from 0.396 in 2015 to 0.735 in 2018) and between IFER and the presence of beauvericin (the R value ranged from 0.364 in 2015 to 0.785 in 2017). The negative correlation between (IFER) and amylose content (ranging from R = −0.303 to R= −0.180) stresses the role of the endosperm starch composition in the kernel resistance to Fusarium ear rot. The conducted study indicated that the risk of kernel infection and contamination with fungal metabolites (beauvericin and ergosterol) was associated with the maize type kernels

Antiviral Activity of Selected Essential Oils against Cucumber Mosaic Virus

The aim of the study was to assess the antiviral activity of selected essential oils (EOs) against Cucumber mosaic virus (CMV), both in vitro and in vivo. The observations were made using Chenopodium quinoa as a local host. The EOs were obtained from Greek oregano, thyme, and costmary. Their chemical composition was determined using GC/FID followed by GC/MS. The dominant compound in oregano EO was carvacrol (59.41%), in thyme EO—thymol (59.34%), and in costmary EO—β-thujone (90.60%). Among the analysed EOs, thyme EO exhibited the most promising effects against CMV. However, its activity was influenced by the time of application. In an in vivo experiment, thyme EO showed protective (pre-inoculation) rather than curative (post-inoculation) activity