Application of repetitively pulsed X-ray radiation in experimental oncology

Development of new technologies in the field of radiation required new approaches and strategies for their application. Power radiation when one continued pulsed divided to serial pulses with different specific repetition rate could provide more complicated and expressed reaction of the biological objects. We used different normal and tumor cell lines in vivo and in vitro to compare efficacy of different pulse repetition rate of X-ray radiation when the total absorbed dose didn’t exceed 1 Gy. We observed strong dependent of tumor cell reaction to repetition rate. Using this parameter we can stimulate or inhibit tumor growth up to 90% compare to control group. Irradiation of tumor-bearing mice inhibited growth of primary tumor up to 60% with the total absorbed dose 0.4 Gy. Moreover same experimental conditions allowed to reduce number of metastasis in mouse lung at 70%. That resulted in longer survival of experimental animals compare to control group. Thus we can conclude that pulsed radiation with nanosecond pulse duration has a potential for application in oncology

Effect of atmospheric-pressure plasma jet on normal and tumor cells in vitro

The purpose of this work is to investigate the effect of low-temperature plasma on tumor and normal cells. As a result of in vitro experiments, plasma-exposed tumor and normal cells demonstrate several effects such as cell detachment, apoptosis or necrosis according to cell type and exposure parameters (power, time of exposure, frequency). In experiments, the inhibition of tumor cell growth was observed up to 70% on the 5th day after exposure. The effect of gas discharge plasma on normal cells was the opposite, and by 5 days there was a stimulation of cell proliferation. The obtained data demonstrate the prospects of using this atmospheric-pressure plasma jet in biomedical research aimed at the treatment of cancer

Different sensitivity of normal and tumor cells to pulsed radiofrequency exposure

The effect of nanosecond radiofrequency pulses (nsRF) on tumor and normal cells has been studied. To determine the viability of cells, an MTT test was used, as well as a real time system for analyzing cell cultures-iCELLigence. It has been shown that ns RF pulses under certain combinations of operating conditions reduce cell proliferation of both tumor and normal cells. Double exposure to 1000 pulses leads to the most effective inhibition of tumor cell proliferation and was 40% after 5 days. Inhibition of the proliferative activity of normal cells was 10% and was maximum after 3 days, then cell growth resumed. The results obtained allow to consider ns RF pulses with different parameters as a promising effective factor for controlling cellular processes for biomedical purposes