51 research outputs found
Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365
Lanthipeptides are ribosomally synthesized and post-translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5- and 5.5-fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster
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Minimum Information about a Biosynthetic Gene cluster
A wide variety of enzymatic pathways that produce specialized metabolites in bacteria, fungi and plants are known to be encoded in biosynthetic gene clusters. Information about these clusters, pathways and metabolites is currently dispersed throughout the literature, making it difficult to exploit. To facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters, we propose the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard.Chemistry and Chemical Biolog
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The biosynthetic implications of late-stage condensation domain selectivity during glycopeptide antibiotic biosynthesis
Regulation of teicoplanin biosynthesis: refining the roles of tei cluster-situated regulatory genes
Activation and Identification of a Griseusin Cluster in Streptomyces sp. CA-256286 by Employing Transcriptional Regulators and Multi-Omics Methods
Actinomycetes: The Antibiotics Producers
Actinomycetes are well known as an inexhaustible source for antibiotics [...
An isotopic probe to follow the stereochemical course of dehydratase reactions in polyketide and fatty acid biosynthesis
Diversity of peptidoglycan structure-Modifications and their physiological role in resistance in antibiotic producers
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