Additional file 1 of A systematic literature review on the health-related quality of life and economic burden of Fabry disease

Additional file 1. Supplementary Tables

Primers and pDONR vectors used in the generation of pENTR vectors.

<p>Primers and pDONR vectors used in the generation of pENTR vectors.</p

Generation of pMULTIrec.

<p><b>A.</b> Four multisite Gateway compatible fragments (eGFPCre; IRES; puro^R and pA) were PCR amplified and cloned into the appropriate pDONR vector to make four pENTR vectors. <b>B.</b> The four pENTR clones were combined into one four-fragment Gateway clone. <b>C.</b> The four-fragment cassette was moved to pDONR/Zeo to change antibiotic resistance and subsequently sub-cloned to a vector carrying a Gateway ENTR cassette upstream of a dual selection (Neo/Kan) cassette. <b>D.</b> The resulting pMULTIrec vector.</p

The <i>Six2</i>^+/GCiP allele based on the pMULTIrec system.

<p><b>A.</b> Retrival of the <i>Six2</i>-GCIP targeting vector. <b>B.</b> Targeting of the <i>Six2</i> locus. <b>C.</b> Confirmation of correct targeting of the <i>Six2</i> locus (restriction enzyme and probe indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062054#pone-0062054-g004" target="_blank">figure 4B</a>). <b>D.. </b><i>Six2</i>^+/GCiP<i>Rosa26</i>^tdRFP kidney in culture showing GFP and RFP signals. <b>E.. </b><i>Six2</i>^+/GCiP<i>Rosa26</i>^tdRFP kidney in culture showing GFP and RFP signals and Cdh1 antibody staining. CM: cap mesenchyme; UB: ureteric bud; N: nephron.</p

The <i>Six2</i>-GCiP BAC construct made using pMULTIrec.

<p><b>A.</b> The four-fragment and dual selection cassette from pMULTIrec was cloned in a <i>Six2</i> containing BAC replacing the start codon. <b>B.</b> GFP imaging of a E13.5 embryo carrying the <i>Six2</i>-GCiP BAC. <b>C.</b> GFP imaging of whole mount E13.5 kidneys. <b>D.</b> E13.5 <i>Six2</i>-GCiP BAC kidneys cultured for four days showing GFP expression in the cap mesenchyme and Cdh1 expression in the ureteric bud.</p

Efficiency of the Gateway reactions used.

<p>Efficiency of the Gateway reactions used.</p

The <i>Nanog</i>-KiP BAC made from a modified pMULTIrec vector.

<p><b>A.</b> Generation of the <i>Nanog</i>-KiP BAC. <b>B, C.</b> mKate2 (B) and brightfield signals of iPS clones derived from <i>Nanog</i>-KiP BAC MEFs. <b>D, E.</b> mKate2 (B) and brightfield signals of iPS clones derived from wild type MEFs.</p

Efficiency of the recombineering reactions used.

<p>Efficiency of the recombineering reactions used.</p