Visualization of Amplified DNA of Origin of Oil Palm (Elaeis guineensis Jacq.) Germplasm PT. Socfindo Based on Three SSR Primers

ABSTRACT   The oil palm plant is a one-piece plant included in Palmae's family. Molecular marking applications can be used to improve efficiency in analyzing genetic relationships, gene mapping, and marker-assisted selection (MAS) in oil palm plants. This study aims to determine the visualization of the amplification of DNA of Origin of Oil Palm (Elaeis guineensis Jacq.) Germplasm PT. Socfindo Based on Three SSR (Simple Sequence Repeats) Primers. The material analyzed is the 14 origin of oil palm originating from the germplasm of PT. Socfindo. The results showed the size of DNA bands that varied from 138 bp to 308 bp. The lowest band size was found on the FR-0782 primers, namely 138-211 bp and the polymorphic percentage was 60%

The effect of drought stress on root morphology of several red rice genotypes (Oryza sativa L.) in the vegetative phase

This study aims to determine the effect of drought stress on root morphology, namely root length, root volume, and dry root weight of red rice on the vegetative phase. This research began in July-October 2018 in the Greenhouse area of ​​the Faculty of Agriculture, Universitas Sumatera Utara, Medan. This study used a randomized block design (RBD) with 2 treatment factors, namely, genotype consisting of 5 red rice genotypes (Inpago 7 variety, B11908D-MR-2-2-4 strain, B11423G-MR-17 strain, B11186G-MR-3-1-18-1 strain, and G15175C-TB-13 strain) and watering frequency consisting of 4 levels of treatment (every day, once every 5 days, once every 10 days and 15 days) with 5 replications. The results showed that the genotypes of red rice showed significant differences in root length, root volume, and dry weight of root. B1186G-MR-3-1-18-1 strain has the highest average root length (42.29 cm), root volume (29.63 ml), and dry weight of root  (6.46 g) compared to other strains. Watering frequency of every 15 days shows the highest root length average (42.51 cm), watering frequency of every 5 days shows the highest root height average (24.60 ml), dry weight of root (5.21 g), and interaction between the genotypes of red rice and the frequency of watering does not show a significant effect

Pola Sebaran Karakter - Karakter Pertumbuhan Dan Produksi Kedelai (Glycine max L. Merril) Hasil Persilangan Grobogan Dengan Genotipa Tahan Salin Pada Turunan F2: Distribution pattern of growth and production characters of hybrid soybean (glycine max L. Merrill) varieties of soybean genotypes resistant Grobogan with saline at F2

Pola Sebaran karakter-karakter pertumbuhan dan produksi kedelai (glycine max L. Merril) hasil persilangan grobogan dengan genotipa tahan salin pada turunan F2, dibimbing oleh Rosmayati dan Eva Sartini Bayu.Tujuan penelitian ini adalah untuk mengevaluasi karakter pertumbuhan dan produksi tanaman kedelai hasil persilangan pada tanah salin. Penelitian ini dilakukan di dalam rumah kasa Fakultas Pertanian USU (± 25 meter dpl) pada bulan April sampai bulan Agustus 2015. Benih F2 diperoleh dari penelitian sebelumnya dengan menggunakan benih hasil persilangan varietas grobogan dengan genotipa kedelai tahan salin. Berdasarkan hasil penelitian untuk seluruh hasil persilangan didapatkan bahwa untuk karakter jumlah daun, umur berbunga, jumlah polong, jumlah polong berisi, jumlah polong hampa, bobot biji, jumlah biji, dan umur panen tidak ada yang berdistribusi normal. Semua karakter tersebut menunjukan sebaran yang tidak normal yang dipengaruhi oleh adanya gen aditif epistasis duplikat maupun komplementer   Distribution pattern of growth and production characters of hybrid soybean (glycine max L. Merrill) varieties of soybean genotypes resistant Grobogan with saline at F2, guided by Rosmayati and Eva Sartini Bayu. The purpose of this study was to evaluate the character of the growth and production of soybean plants from F2 in saline soil. This research was carried out in home screen of the Faculty of Agriculture USU (± 25 meters above sea level) in April till August 2015. The F2 seeds obtained from previous studies using Grobogan varieties of seeds from crosses with saline resistant soybean genotypes. The results showed that for plant height, number of leaves, number of branches, flowering dates, number of pods containing the number of empty pods, seed weight, number of seeds, and harvesting is not normally distributed. All of these characters shows the distribution is not normal is influenced by the presence of an additive gene duplicates or complementary epistasis

Penampilan Morfologi Akar Beberapa Hasil Persilangan (F1) Tanaman Jagung pada Media Tanam Tanah Gambut dengan Penambahan Bahan Organik Leguminosa di Rhizotron: Morphological Root Performance of Hybrid Maize (F1) in Peat Soil Medium Applicated with Legume Organic Manure in Rhizotron

The maize in indonesia mostly use land that is not fertile. The objective of the research was identified the selection character based on root morphology of hybrid maize (F1) in peat soil medium applicated with legume organic manure in rhizotron. The research was conducted at the greenhouse of Faculty of Agriculture, University of Sumatera Utara, from February until July 2017. The Randomized Block Design was used with two factors, the first factor is the population (V1, V2, V3, V4, V5) and the second factor is the planting medium (peat soil, peat soil + leucena, peat soil + mucuna). The parameters observed were: the number of root, the length of root, the diameter of root dispersed, the fresh weight of the canopy, the fresh weight of root and the dry weight of root. The result showed that the population F1 significantly affected the number of root, the volume of root, the fresh weight of root and the dry weight of root. The planting medium significantly affected the number of root, the length of root, the diameter of root dispersed, the volume of root, the fresh weight of the canopy, the fresh weight of root, the dry weight of root. Interaction between the population F1 NEI9008 x CLA46 (V5) and the planting medium peat soil + mucuna significantly affected the fresh weight of root. Tanaman jagung di Indonesia banyak menggunakan lahan yang tidak subur.Penelitian ini bertujuan untuk menduga karakter seleksi morfologi akar tanaman jagung dari beberapa hasil persilangan (F1) terhadap pemberian bahan organik leguminosa pada media tanam tanah gambut di rhizotron. Penelitian ini dilaksanakan di rumah kaca Fakultas Pertanian Universitas Sumatera Utara Medan, pada bulan Februari sampai dengan Juli 2017. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 2 faktor perlakuan yaitu. Populasi (V1, V2, V3, V4, V5) sebagai faktor pertama dan media tanam (gambut, gambut+bo lamtoro dan gambut+bo mucuna) sebagai faktor kedua. Peubah amatan yang diamati adalah jumlah akar, panjang akar, volume akar, diameter sebaran akar, bobot basah tajuk, bobot kering tajuk, bobot basah akar, dan bobot kering akar. Hasil penelitian menunjukkan populasi F1 berpengaruh nyata pada tinggi tanaman, jumlah akar, volume akar, bobot basah akar, and bobot kering akar. Media tanam berpengaruh sangat nyata pada jumlah akar, panjang akar, diameter sebaran akar, volume akar, bobot basah tajuk, bobot kering tajuk, bobot basah akar, bobot kering akar. Interaksi antara populasi F1 NEI9008 x CLA46 (V5) dengan media tanam gambut + bo mucuna berpengaruh nyata pada bobot basah akar

Application of Chopper Machinery Technology from Oil Palm Fronds in Huta Gondang Rejo Nagori Bandar Tongah Bandar Hands, Regency of Simalungun

This community service activity aims to improve the efficiency of cattle farm business in Huta Gondang Rejo Nagori Bandar Tongah Bandar Huluan Subdistrict Simalungun Regency through the use of pellet complete feed based on palm oil fronds and agricultural waste. This activity is conducted on cattle ranchers who are members of the farming community group Huta Gondang Rejo. Farmers are given counseling and training on the processing of palm oil fronds and agricultural waste into livestock feed and the establishment of a complete ration of palm-based pellet and agricultural waste. To facilitate the transfer of this technology to the breeder, then prepared a complete feed pellet with 5 types of machines. Palm Crusher Counter Machines or Palm Crafter Engines with a capacity of 600 kg/hour. Pellet printing machine (granulator) with capacity of 100 kg/hour. Mixer machine (mixer feed) with a capacity of 50 kg/stir. Dryer (Oven) with capacity of 10 kg/rack. Manual press feed press tool with specification 2 kg/print. Measuring the success of this activity is seen from the level of farmer adoption of pelleting technology and the difference of ration conversion between cattle that get complete feed and conventional or traditional. Performance of palm cropping machine for cattle that get complete pellet feed is better than cattle that get conventional or traditional feed. This Chopper machine can count the palm stem from the tip of the base of the leaf to the stem (80% of the palm stem). Through the activities of plant waste feed technology is expected to achieve some outcomes, namely, improve the productivity of farming through the system integration of livestock combine farming system with synergistic system to form an effective, efficient and environmentally friendly.&nbsp

Growth and Production Response of Varieties of Mung Beans (Vigna radiata L.) to Gibberellin Concentration in the Saline Field

This study aims to determine the growth and production response of some mung bean varieties with the application of gibberellin at different concentrations in the saline field. This research was conducted at Hamparan Perak , District of Deli Serdang North Sumatera, Medan (± 3-9 m asl) from May - August 2017. The tretment was arranged by Randomized Block Design (RBD) with two factors, three mung green varieties (Vima-1, Vima-2, and Vima-3) and GA3 concentration (0 ppm, 100 ppm, 200 ppm). The results showed that there were significant diffrent among varieties on plant height, the number of pods per plant, and the diameter of the seed. Gibberellin concentration gives sinificant effect to the plant height in 3 and 5 week after pllanted, root volume, and seed diameter. The interaction between aplication gibberellins and varieties had a significant effect on the root volume

Respons Pertumbuhan dan Produksi Kedelai (Glycine max (L.) Merril.) Terhadap Aplikasi Pupuk Hayati dan Tepung Cangkang Telur : The Growth Response and Production of Soybean (Glycine max (L.) Merril.) on Biological Fertilizer and Eggshell Powder

Penelitian ini dilakukan di lahan percobaan Fakultas Pertanian Universitas Sumatera Utara yang berada ± 25 m dpl mulai Mei sampai Agustus 2015. Tujuan penelitian ini yaitu untuk mengetahui kemampuan pupuk hayati dan tepung cangkang telur dalam meningkatkan pertumbuhan dan produksi tanaman kedelai. Penelitian menggunakan Rancangan Acak Kelompok dengan 2 faktor perlakuan. Faktor pertama adalah pemberian pupuk hayati yaitu 0 ; 5 dan 10 g/kg benih dan faktor kedua tepung cangkang telur yaitu 0 ; 25 ; 50 dan 75 g/polibeg. Hasil penelitian menunjukkan bahwa pemberian pupuk hayati meningkatkan jumlah dan berat bintil akar efektif dan bobot biji kering per tanaman. Sedangkan perlakuan tepung cangkang telur nyata dalam meningkatkan jumlah dan bobot bintil akar efektif. Sedangkan interaksi antara pupuk hayati dan tepung cangkang telur cenderung meningkatkan jumlah dan berat bintil akar efektif dan bobot biji kering per tanaman.   This research was conducted at the experimental field of Faculty of Agriculture, University of Sumatera Utara located ± 25 m above sea level on May to August 2015. The objective of the research was to determine the ability of biological fertilizer and eggshell powder on growth and production of soybean. This research using Randomized Block Design with 2 factors. The first factor was application of biological fertilizer ie (0 ; 5 and 10 g/kg seeds ) and the second factors was eggshell powder ie (0 ; 25 ; 50 and 75 g/plant ). This research showed that application of eggshell powder increased the number and weight of effective nodule and dry seeds weight per plant. While the biological fertilizer treatment significantly increased the number and weight of effective nodule. The interaction among eggshell powder and biological fertilizer tended to increase number and weight of effective nodule and dry seeds weight per plant

Identifikasi Karakter Morfologis Dan Hubungan Kekerabatan Bawang Merah (Allium Ascalonicuml) Di Kabupaten Samosir : Identification of morphological character of shallot (Allium asscalonicum L) in Samosir district

This reserach aimed to identify the morphological chracteristics and phylogenetic relationship of shallot in five region of Samosir distric from September to April 2017 by survey method. Shallot samples founded in five districs were identified and characterzed based on International Plant Genetic Resources Institute (IPGRI) standard. Sampling technique using accidental sampling. Parameters observed were plant height, number of leaves, foliage cracking, foliage colour, foliage attitude,number of tillers, number of bulbs, pupulation of bulbs, shape of bulbs, colour of bulbs and bulbs widht. The result showed that there were 18 accession of shallot found in five districts. Morphologycal characteristics of shallot are foliage colours (green and grey-green), folliage attitude (intermediate and erect), folliage cracking (weak,mediu and strong) and shape of bulbs (globe, broad elliptic, broad oval and spindle). The closest relationship is B6 and B17, B3 and B5, B1 and B2 with a coefecient dissimilarity 0,00 and farthest relationship is B16 and G13 with coefecient dissimilarity 25.95%.   Identifikasi Karakter Morfologis dan Hubungan Kekerabatan Bawang Merah (Allium ascalonicum L.)) di Beberapa Kabupaten Samosir. Dibimbing oleh Ir. Eva Sartini Bayu, MP. dan Ir. Mbue Kata Bangun M.S. Penelitian ini bertujuan untuk mengidentifikasi karakter morfologis dan hubungan kekerabatan tanaman bawang merah yang ada di Kabupaten Samosir. Penelitian ini dilakukan di 5 kecamatan di kabupaten samosir pada bulan SeptemberApril 2017 dengan metode survei menggunakan panduan deskriptor bawang merah IPGRI. Teknik pengambilan sampel secara accidental sampling. Parameter yang diamati adalah tinggi tanaman, Jumlah daun, tekstur daun, warna daun, posisi daun, jumlah anakan, jumlah umbi, populasi umbi, bentuk umbi, warna umbi dan diameter umbi. Hasil penelitian menunjukkan bahwa di Kabupaten Samosir terdapat 18 aksesi bawang merah yang diidentifikasi. Hasil karakteristik morfologis untuk warna daun (hijau dan hijau tua), posisi daun (tengah dan tegak lurus), tekstur daun (lemah,sedang,kuat), bentuk umbi (bundar, lonjong melebar, Oval melebar, lonjong). Hubungan kekerabatan terdekat pada aksesi B6 dan B17, B3 dan B5, B1 dan B2 dengan koefisien ketidaksamaan sebesar 0,00 dan hubungan kekerabatan terjauh pada aksesi B16 dan B13 dengan koefisien ketidaksamaan sebesar 25,9%.

Keragaan Morfologi dan Kandungan Antosianin Padi Beras Merah (Oryza sativa L.) di Kecamatan Munte dan Kecamatan Payung, Kabupaten Karo

Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g