Map Resources – ECO Farms in the Czech Republic

The present paper deals with the creation of maps for the sake of a special map portal using the database of eco farms in the Czech Republic. The map output is being developed and implemented within the framework of the Research Program in mutual cooperation of the Department of Information Technologies, Information and Consulting Centre, other departments of the Faculty of Economics and Management CULS Prague with external partners, especially with the Ministry of Agriculture (Environment and Ecological Agriculture Section, Department of Ecological Agriculture) and the Institute of Agricultural Economics and Information (UZEI) - Division of Agro-environmental Policy in Brno. The maps are visualized by means of MPPR 1.0 system and have been processed – in the pilot stage - for the South Bohemian Region eco farms. The results represent initial data from 2009 (as at 31st December 2009) that have been processed, verified and complemented in the course of 2010

Organic farms in the Czech Republic – Map Portal presentation opportunities

The paper is aimed at presenting the map portal of organic farms in the Czech Republic. The pilot project is concerned with the South Bohemia Region. Extensive map data and resources are displayed by means of a purpose-developed universal software solution called Regional Development Map Portal (RDMP) version 1.0. The database was generated and updated on the basis of detailed content validation and strives for maximum accuracy of map object location. The software solution – apart from supporting all standard functions – represents qualitatively a brand new perspective of map data creation and entails many original elements and functionalities

Map Resources – ECO Farms in the Czech Republic

The present paper deals with the creation of maps for the sake of a special map portal using the database of eco farms in the Czech Republic. The map output is being developed and implemented within the framework of the Research Program in mutual cooperation of the Department of Information Technologies, Information and Consulting Centre, other departments of the Faculty of Economics and Management CULS Prague with external partners, especially with the Ministry of Agriculture (Environment and Ecological Agriculture Section, Department of Ecological Agriculture) and the Institute of Agricultural Economics and Information (UZEI) - Division of Agro-environmental Policy in Brno. The maps are visualized by means of MPPR 1.0 system and have been processed – in the pilot stage - for the South Bohemian Region eco farms. The results represent initial data from 2009 (as at 31st December 2009) that have been processed, verified and complemented in the course of 2010.Map portal, web browser, Google Maps, eco farm, ecological agriculture, Act No. 242/2000 Coll, Agricultural and Food Policy, Community/Rural/Urban Development, Farm Management, GA, IN,

Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

<p>Abstract</p> <p>Background</p> <p>Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification of proteins resembling the tumor biology can improve the diagnosis, prediction, treatment selection, and targeting of therapy. Since the beginning of the post-genomic era, the focus of molecular biology gradually moved from genomes to proteins and proteomes and to their functionality. Proteomics can potentially capture dynamic changes in protein expression integrating both genetic and epigenetic influences.</p> <p>Methods</p> <p>We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis. Seven patients developed distant metastases within three-year follow-up. These samples were included into a metastase-positive group, the others formed a metastase-negative group. Two-dimensional electrophoretical (2-DE) gels in pH range 4–7 were prepared. Spot densities in 2-DE protein maps were subjected to statistical analyses (R/maanova package) and data-mining analysis (GUHA). For identification of proteins in selected spots, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.</p> <p>Results</p> <p>Three protein spots were significantly altered between the metastatic and non-metastatic groups. The correlations were proven at the 0.05 significance level. Nucleophosmin was increased in the group with metastases. The levels of 2,3-trans-enoyl-CoA isomerase and glutathione peroxidase 1 were decreased.</p> <p>Conclusion</p> <p>We have performed an extensive proteomic study of mammary epithelial cells from breast cancer patients. We have found differentially expressed proteins between the samples from metastase-positive and metastase-negative patient groups.</p

Sex-specific pathways in early cardiac response to pressure overload in mice

Pressure overload (PO) first causes cardiac hypertrophy and then heart failure (HF), which are associated with sex differences in cardiac morphology and function. We aimed to identify genes that may cause HF-related sex differences. We used a transverse aortic constriction (TAC) mouse model leading to hypertrophy without sex differences in cardiac function after 2 weeks, but with sex differences in hypertrophy 6 and 9 weeks after TAC. Cardiac gene expression was analyzed 2 weeks after surgery. Deregulated genes were classified into functional gene ontology (GO) categories and used for pathway analysis. Classical marker genes of hypertrophy were similarly upregulated in both sexes (α-actin, ANP, BNP, CTGF). Thirty-five genes controlling mitochondrial function (PGC-1, cytochrome oxidase, carnitine palmitoyl transferase, acyl-CoA dehydrogenase, pyruvate dehydrogenase kinase) had lower expression in males compared to females after TAC. Genes encoding ribosomal proteins and genes associated with extracellular matrix remodeling exhibited relative higher expression in males (collagen 3, matrix metalloproteinase 2, TIMP2, and TGFβ2, all about twofold) after TAC. We confirmed 87% of the gene expression by real-time polymerase chain reaction. By GO classification, female-specific genes were related to mitochondria and metabolism and males to matrix and biosynthesis. Promoter studies confirmed the upregulation of PGC-1 by E2. Less downregulation of metabolic genes in female hearts and increased protein synthesis capacity and deregulation of matrix remodeling in male hearts characterize the sex-specific early response to PO. These differences could contribute to subsequent sex differences in cardiac function and HF

Hidden socio-environmental costs of free trade : the case of shrimp pre-processing industry in Kerala, India

This thesis examines the relation between free trade and socio-environmental costs, which are due to their less visible character and difficulty to be captured excluded from accounting. On the background of emerging trade liberalization, this paper reviews how the concept of costs that are related to damages to the environment and decreased human well-being evolved during the last century, and how different frameworks made attempt to capture them. Kapp’s framework that strictly distinguishes between social and environmental costs is applied to the case study of shrimp pre-processing industry in Ambalappuzha, small fishery village in Kerala. Using both qualitative methods of interviews and field observations, and quantitative methods for collecting data by questionnaires, the research uncovers the main socio-environmental costs that are born mainly by poor women from low social strata with limited access to information. These women lack power to avoid health problems and damages of air and water pollution caused by the industry. The direction of the shifted costs points at developed countries that have been taking advantage on the less developed ones by systematically incorporating these failures in their economies. The economic system itself is being suspected for its rigidity, the market failure may not necessary be accidental, but intentional as the entire economic system relies on them.M-IE