Evaluation of cytotoxicity and wound healing activity of Avicennia schaueriana in cream

Abstract:Avicennia schaueriana is found in Brazilian mangrove coast. The cytotoxicity in vitro of the Aqueous Extract of Leaves of Avicennia schaueriana (AELAs) and the healing activity of the plant in cream on mice skin wounds were evaluated in this study. The cytotoxic evaluation was performed on Vero cells. The healing activity was evaluated on mice treated during 5, 10 and 15 days with cream at 5%, solution of sodium chloride at 0.9% and dexpanthenol in cream at 5%. The extract did not show cytotoxicity, but showed mitogenic activity (100μg/ml). In morphometric analysis, the percentage of wound contraction after 10 days was higher in dexpanthenol group (93.41%). In 15 days, the lowest percentage of contraction was observed in the dexpanthenol group (94.41%) and the highest in the AELAs cream group (98.50%). In histomorphometry the dexpanthenol showed the lowest length of re-epithelialization in 10 days. In 15 days, the AELAs cream group showed 100% of re-epithelialization. The number of fibroblasts found in AELAs cream group was higher than the saline solution in 10 days. In 15 days, AELAs cream group maintained a higher amount of fibroblasts when compared to the others. A. schaueriana did not show cytotoxicity. Furthermore, topical application of AELAs cream decreased the wound area, stimulated the re-epithelialization and increased the number of fibroblasts. The species A. schaueriana could become a topical treatment in tissue repair process

Healing activity and pre-clinical toxicological study of phytotherapic sanativo®

A atividade cicatrizante de ferida do Sanativo® (SAN) e os possíveis efeitos tóxicos da sua administração subcrônica foram avaliados em ratos. O SAN reduziu significativamente a área das feridas abertas no dorso dos ratos. Na toxicidade subcrônica, o SAN na dose de 1,675 g/kg reduziu significativamente o ganho de massa corporal das ratas apenas na primeira semana de administração. O SAN produziu alterações significativas pontuais nos parâmetros hematológicos e bioquímicos, embora dentro da faixa de referência para espécie. A análise histológica dos tecidos não mostrou alteração. Em conclusão, o Sanativo® possui significativa propriedade cicatrizante no modelo avaliado e baixa toxicidade em ratos Wistar.The wound healing activity of phytotherapic Sanativo®(SAN) and the possible hazard effects of the subchronic administration of SAN were evaluated in rats. SAN induced significant decrease in the area of the open wound in the rat dorsum. In the sub-chronic toxicity studies, SAN in doses of 1,675 g/kg, decreased the gain in body mass, but this was significant only in the first week of administration. SAN produced only a few significant alterations in the hematological and biochemical parameters, although without dose-response correlation and were within the parameters of this species. The histological analysis of the tissues did not show changes. In conclusion, Sanativo ® possesses significant healing property in the open wound model and low toxicity in Wistar rats.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Healing activity and pre-clinical toxicological study of phytotherapic sanativo®

A atividade cicatrizante de ferida do Sanativo® (SAN) e os possíveis efeitos tóxicos da sua administração subcrônica foram avaliados em ratos. O SAN reduziu significativamente a área das feridas abertas no dorso dos ratos. Na toxicidade subcrônica, o SAN na dose de 1,675 g/kg reduziu significativamente o ganho de massa corporal das ratas apenas na primeira semana de administração. O SAN produziu alterações significativas pontuais nos parâmetros hematológicos e bioquímicos, embora dentro da faixa de referência para espécie. A análise histológica dos tecidos não mostrou alteração. Em conclusão, o Sanativo® possui significativa propriedade cicatrizante no modelo avaliado e baixa toxicidade em ratos Wistar.The wound healing activity of phytotherapic Sanativo®(SAN) and the possible hazard effects of the subchronic administration of SAN were evaluated in rats. SAN induced significant decrease in the area of the open wound in the rat dorsum. In the sub-chronic toxicity studies, SAN in doses of 1,675 g/kg, decreased the gain in body mass, but this was significant only in the first week of administration. SAN produced only a few significant alterations in the hematological and biochemical parameters, although without dose-response correlation and were within the parameters of this species. The histological analysis of the tissues did not show changes. In conclusion, Sanativo ® possesses significant healing property in the open wound model and low toxicity in Wistar rats.Colegio de Farmacéuticos de la Provincia de Buenos Aire