Heat shock factor 1 mediates the longevity conferred by inhibition of TOR and insulin/IGF-1 signaling pathways in C. elegans

Target of rapamycin (TOR) signaling is an evolutionarily well-conserved pathway that regulates various physiologic processes, including aging and metabolism. One of the key downstream components of TOR signaling is ribosomal protein S6 kinase (S6K) whose inhibition extends the lifespan of yeast, Caenorhabditis elegans, Drosophila, and mice. Here, we demonstrate that the activation of heat shock factor 1 (HSF-1), a crucial longevity transcription factor known to act downstream of the insulin/IGF-1 signaling (IIS) pathway, mediates the prolonged lifespan conferred by mutations in C.elegans S6K (rsks-1). We found that hsf-1 is required for the longevity caused by down-regulation of components in TOR signaling pathways, including TOR and S6K. The induction of a small heat-shock protein hsp-16, a transcriptional target of HSF-1, mediates the long lifespan of rsks-1 mutants. Moreover, we show that synergistic activation of HSF-1 is required for the further enhanced longevity caused by simultaneous down-regulation of TOR and IIS pathways. Our findings suggest that HSF-1 acts as an essential longevity factor that intersects both IIS and TOR signaling pathways.X1144sciescopu

Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish.

Although atherosclerosis is a multifactorial disease, the role of hemodynamic information has become more important. Low and oscillating wall shear stress (WSS) that changes its direction is associated with the early stage of atherosclerosis. Several in vitro and in vivo models were proposed to reveal the relation between the WSS and the early atherosclerosis. However, these models possess technical limitations in mimicking real physiological conditions and monitoring the developmental course of the early atherosclerosis. In this study, a hypercholesterolaemic zebrafish model is proposed as a novel experimental model to resolve these limitations. Zebrafish larvae are optically transparent, which enables temporal observation of pathological variations under in vivo condition. WSS in blood vessels of 15 days post-fertilisation zebrafish was measured using a micro particle image velocimetry (PIV) technique, and spatial distribution of lipid deposition inside the model was quantitatively investigated after feeding high cholesterol diet for 10 days. Lipids were mainly deposited in blood vessel of low WSS. The oscillating WSS was not induced by the blood flows in zebrafish models. The present hypercholesterolaemic zebrafish would be used as a potentially useful model for in vivo study about the effects of low WSS in the early atherosclerosis

Effects of surface morphological structure of a brown alga miyeok (Undaria pinnatifida) on sustainable drag reduction

Liquid-infused surfaces are a promising passive technique for frictional drag reduction. However, their practical application is limited by the easy depletion of the lubricant infused in the surface by external forces. Miyeok contains mucus on its surface, which induces a sustainable slippery surface under flow conditions. In this study, the effect of the surface morphological structures of miyeok on slippery characteristics was examined. The surface of miyeok has bump-like microstructures and tiny pores of mucilage gland cells with a re-entrant inlet configuration. A replica model of the miyeok surface was fabricated by adopting a molding process using polydimethylsiloxane. The miyeok replica model was dipped into a silicone oil solution to obtain a slippery surface property. The surface structures of fresh miyeok and the replica were checked by comparing their scanning electron microscopy images. A replica of a kelp surface was also fabricated by employing the same method. The heights of the surface microstructures of the miyeok replica were more uniform than those of the kelp replica. The sustainable drag reduction effect of the replica models' surfaces was assessed by a sessile drop method. The liquid-infused miyeok replica surface (LIMRS) showed better sustainability than other surfaces. The slippery properties of the LIMRS were examined by measuring slip length and pressure drop. The drag reduction rates measured by the two methods were approximately 24% and 19%. The present results would be helpful for understanding the effects of the surface structure of natural miyeok on sustainable drag reduction and developing a biomimetic sustainable drag-reducing surface.11Ysciescopu

지속 가능한 항력 저감을 위한 해조류 미역의 형태학적 미끄럼 구조

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미역의 형태학적 표면 구조가 지속가능한 항력감소에 미치는 효과 연구

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A nature-inspired lubricant-infused surface for sustainable drag reduction (vol 15, pg 8459, 2019)

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