1 research outputs found
Effectiveness of Periodic Treatment of Quercetin against Influenza A Virus H1N1 through Modulation of Protein Expression
Kimchi, a traditional
fermented food regularly consumed in Korea,
contains various types of antimicrobial compounds. Among the tested
compounds present in common spices used in Kimchi, quercetin showed
the highest selectivity index against influenza A virus (IAV) H1N1.
In this study, the effect of pretreatment and periodic treatment with
quercetin against IAV in Madin–Darby canine kidney cells was
observed. Compared to pretreatment, periodic treatment resulted in
significantly higher cell viability but lower relative expression
of the IAV PA gene and total apoptosis and cell death. To explain
the mechanisms underlying the antiviral effects of quercetin treatment,
a comparative proteomic analysis was performed in four samples (mock,
quercetin-treated, IAV-infected, and quercetin-treated IAV-infected).
Among the 220 proteins, 56 proteins were classified nonhierarchically
into three clusters and were differentially modulated by quercetin
treatment in IAV-infected cells. Post-translational modifications
were identified in 68 proteins. In conclusion, periodic treatment
with quercetin is effective in reducing IAV infection, and differentially
regulates the expression of key proteins, including heat shock proteins,
fibronectin 1, and prohibitin to reduce IAV replication