1 research outputs found
Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis
The parallelization of microfluidic cytometry is expected
to lead
to considerably enhanced throughput enabling point-of-care diagnosis.
In this article, the development of a microfluidic potentiometric
multichannel cytometer is presented. Parallelized microfluidic channels
sharing a fluid path inevitably suffer from interchannel signal crosstalk
that results from electrical coupling within the microfluidic channel
network. By employing three planar electrodes within a single detection
channel, we electrically decoupled each channel unit, thereby enabling
parallel analysis by using a single cytometer microchip with multiple
microfluidic channels. The triple-electrode configuration is validated
by analyzing the size and concentration of polystyrene microbeads
(diameters: 1.99, 2.58, 3, and 3.68 μm; concentration range:
∼2 × 10<sup>5</sup> mL<sup>–1</sup> to ∼1
× 10<sup>7</sup> mL<sup>–1</sup>) and bacterial microdispersion
samples (<i>Bacillus subtilis</i>, concentration range:
∼4 × 10<sup>5</sup> CFU mL<sup>–1</sup> to ∼3
× 10<sup>6</sup> CFU mL<sup>–1</sup>). Crosstalk-free
parallelized analysis is then demonstrated using a 16-channel potentiometric
cytometer (maximum cross-correlation coefficients |<i>r</i>|: < 0.13 in all channel combinations). A detection throughput
of ∼48 000 s<sup>–1</sup> was achieved; the throughout
can be easily increased with the degree of parallelism of a single
microchip without additional technical complexities. Therefore, this
methodology should enable high-throughput and low-cost cytometry