2 research outputs found
Bioavailability of Ginsenosides from White and Red Ginsengs in the Simulated Digestion Model
This study aims to investigate
the bioavailability of ginsenosides
during simulated digestion of white (WG) and red (RG) ginseng powders.
Stability, bioaccessibility, and permeability of ginsenosides present
in WG and RG were studied in a Caco-2 cell culture model coupled with
oral, gastric, and small intestinal simulated digestion. Most ginsenosides
in WG and RG were stable (>90%) during the simulated digestion.
Bioaccessibilities
of total ginsenosides during in vitro digestion of WG and RG were
similar at approximately 85%. However, the bioaccessibility of protopanaxatriol
type ginsenosides in the early food phase was greater than that of
the protopanaxadiol type. The less polar RG ginsenosides were released
later following the jejunum phase. Ginsenosides had low permeability
(<1 × 10<sup>–6</sup> cm/s) through Caco-2 cell monolayers.
These findings suggest that the WG and RG ginsenoside compositions
affect bioaccessibility during digestion and that ginsenosides are
poorly absorbed in humans
Secretome Profiling Reveals the Signaling Molecules of Apoptotic HCT116 Cells Induced by the Dietary Polyacetylene Gymnasterkoreayne B
Dietary polyacetylenes from various
foods have been receiving attention
as promising cancer chemopreventive agents. However, until now, the
detailed molecular mechanism and the regulatory proteins underlying
these effects have not been elucidated. We investigated the effects
of gymnasterkoreayne B (GKB), a model dietary polyacetylene from wild
vegetables, on the programmed cell death of HCT116 human colorectal
cancer cells. GKB inhibited HCT116 cell proliferation by inducing
apoptotic cell death. GKB treatment resulted in ROS accumulation,
leading to the activation of both intrinsic and extrinsic apoptotic
pathway. We also found that FN1, TGFB1, APP, SERPINE1, HSPD1, SOD1,
TXN, and ACTN4 may act as secretory signaling molecules during GKB-induced
apoptotic cell death using LC–MS/MS identification followed
by spectrum counting, statistical calculation, and gene ontology analysis.
The secretory proteins suggested in this study may be promising candidates
involved in apoptotic cell death of cancer cells induced by GKB that
warrant further functional study