Interacción genotipo × ambiente y análisis de estabilidad para rendimiento de materia seca y de semilla en guisante (Pisum sativum L.)]

The objectives of this study were to evaluate dry matter (DM) yield and seed yield of six leafed and semi-leafless pea (Pisum sativum L.) genotypes, and to compare them for these traits. Evaluation of genotype x environment (G x E) interaction, stability and cluster analysis were also carried out at eight diverse locations with typical Mediterranean and Mediterranean-type climate during the 2001-2002 and 2002-2003 growing seasons. Significant differences were found among the pea genotypes for DM and seed yield on individual years and combined over years, and in all locations. All interactions which related to G x E interaction showed significance (P>0.001) for DM and seed yield. The highest yield (4789 kg ha(-1)) was obtained from the leafed genotype 'Urunlu'. However, stability analysis indicated that for DM yield, the leafed genotypes 'Golyazi' and 'Urunlu' should be grown in low yielding and high yielding environments, respectively. Cluster analysis, based on grouping locations, showed that P101 was the preferred variety in low yielding environments, and P98, in high yielding ones. It was suggested that the use of both stability and cluster analyses might give better results. Comparison of cluster and stability analyses showed that the stability analysis fails to recommend cultivars to different regions where yield potential showed significant differences. It seems, however, that cluster analysis could be a powerful tool to examine G x E interaction. If the number of environments was sufficient, a separate stability analysis could be run in each cluster.Uludag University Research FundUludag University [2002/76]The authors wish to thank Prof. Dr. Vincent Pantalone, Plant Sciences Dept., University of Tennessee, USA for critical reading of the manuscript and his valuable suggestions. The Bursa location of this study was supported by Uludag University Research Fund (2002/76)

Dry matter accumulation and forage quality characteristics of different soybean genotypes

Three different soybean [Glycine max(L.) Merr.] cultivars (A-3127, Derry, and Yemsoy) were evaluated for dry matter (DM) yield, plant components, and crop growth rate (CGR) at different row spacings and harvesting stages in 3 locations with Mediterranean- type climate in a split-split plot design with 3 replications in 2009 and 2010. In addition, crude protein (CP), crude protein yield (CPY), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN), and relative feed value (RFV) of harvested forage soybeans were determined at the Bursa (Turkey) location. Increased row spacings significantly reduced DM yield at all locations. Combined over other main effects, the 25-cm row spacing produced 35% more DM yield than the 75-cm row spacing. Row spacings greatly affected DM yield at the R5 and particularly the R7 stage, but this effect was minimal at the R1 stage. The forage-type cultivar Derry produced significantly higher DM yield in all locations, particularly at the R5 and R7 stages. The constituent components of DM were similar at different row spacings of soybean cultivars. In general, the CGR decreased from 25-cm row spacing to 75-cm row spacing in all locations, and the CGR of the cultivars varied significantly according to harvest period, row spacing, and location. Derry had a significantly higher CGR than the other cultivars in the Antalya and Bursa locations. Quality characteristics of soybean forage were not significantly affected by row spacing and showed little effect by cultivar. Harvest stage significantly affected CP, ADF, NDF, TDN, and RFV concentrations of soybean forage. It was concluded that in Mediterranean environments forage-type soybeans (i.e. Derry) can be seeded in narrow rows (25 cm) at high plant population (100 kg ha–1) and offer the most DM yield when harvested at later R stages (e.g., R7) rather than at the early reproductive stage.Three different soybean [Glycine max(L.) Merr.] cultivars (A-3127, Derry, and Yemsoy) were evaluated for dry matter (DM) yield, plant components, and crop growth rate (CGR) at different row spacings and harvesting stages in 3 locations with Mediterranean- type climate in a split-split plot design with 3 replications in 2009 and 2010. In addition, crude protein (CP), crude protein yield (CPY), acid detergent fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDN), and relative feed value (RFV) of harvested forage soybeans were determined at the Bursa (Turkey) location. Increased row spacings significantly reduced DM yield at all locations. Combined over other main effects, the 25-cm row spacing produced 35% more DM yield than the 75-cm row spacing. Row spacings greatly affected DM yield at the R5 and particularly the R7 stage, but this effect was minimal at the R1 stage. The forage-type cultivar Derry produced significantly higher DM yield in all locations, particularly at the R5 and R7 stages. The constituent components of DM were similar at different row spacings of soybean cultivars. In general, the CGR decreased from 25-cm row spacing to 75-cm row spacing in all locations, and the CGR of the cultivars varied significantly according to harvest period, row spacing, and location. Derry had a significantly higher CGR than the other cultivars in the Antalya and Bursa locations. Quality characteristics of soybean forage were not significantly affected by row spacing and showed little effect by cultivar. Harvest stage significantly affected CP, ADF, NDF, TDN, and RFV concentrations of soybean forage. It was concluded that in Mediterranean environments forage-type soybeans (i.e. Derry) can be seeded in narrow rows (25 cm) at high plant population (100 kg ha–1) and offer the most DM yield when harvested at later R stages (e.g., R7) rather than at the early reproductive stage

Analysis of ancient DNA from in vitro

Seven ancient seeds, about 1600 years old, were found during an archaeological excavation in Asar Island which is located in south-western Turkey. These seeds were subjected to germination, in vitro callus induction and molecular characterization experiments to test the viability and plant origin of the seeds. Six of the seven seeds had viable seed components (such as cotyledons) and produced callus tissue in Murashige and Skoog medium supplemented with 2mgl(-1) 6-benzylamino purine (BAP), 0.2 mgl(-1) 1-naphthaleneacetic acid (NAA), 20 gl(-1) sucrose, 2mgl(-1) glycine and 7 gl(-1) agar, but the calli from these seeds failed to yield adventitious shoots. DNA samples from callus tissues produced by ancient seeds in vitro were of good quality. The internal transcribed spacer (ITS) region in nuclear DNA (nDNA) of ancient seeds was amplified successfully. The sequences from amplified ITS DNA products of six ancient seeds indicated that their ITS sequences matched those of Anagyris foetida after subjecting them to BLAST searches in international sequence databases (NCBI). A. foetida is a relict species endemic to the Mediterranean region and used as a herbal medicine. We believe that seed characteristics such as the very hard, extremely smooth and shiny testa, toxic anagyrine alkaloid content and their storage in a pot further improved the longevity of these ancient seeds