Penta­carbonyl-2κ5 C-chlorido-1κCl-bis­[1(η5)-cyclo­penta­dien­yl](μ-α-oxido­benzyl­idene-1:2κ2 O:C)titanium(IV)tungsten(0)

The title compound, [TiW(C5H5)2(C7H5O)Cl(CO)5], consists of two metal centres, with a (tungstenpenta­carbon­yl)oxy­phenyl­carbene unit coordinated by a titanocene chloride. The oxycarbene group is nearly planar, with the phenyl ring twisted by an angle of 39.1 (2)° with respect to this plane. One of the cyclo­penta­dienyl rings undergoes an offset face-to-face π–π inter­action [3.544 (6) Å] with the symmetry-related cyclo­penta­dienyl ring of a neighbouring mol­ecule

Development of a simple artificial intelligence method to accurately subtype breast cancers based on gene expression barcodes

>Magister Scientiae - MScINTRODUCTION: Breast cancer is a highly heterogeneous disease. The complexity of achieving an accurate diagnosis and an effective treatment regimen lies within this heterogeneity. Subtypes of the disease are not simply molecular, i.e. hormone receptor over-expression or absence, but the tumour itself is heterogeneous in terms of tissue of origin, metastases, and histopathological variability. Accurate tumour classification vastly improves treatment decisions, patient outcomes and 5-year survival rates. Gene expression studies aided by transcriptomic technologies such as microarrays and next-generation sequencing (e.g. RNA-Sequencing) have aided oncology researcher and clinician understanding of the complex molecular portraits of malignant breast tumours. Mechanisms governing cancers, which include tumorigenesis, gene fusions, gene over-expression and suppression, cellular process and pathway involvementinvolvement, have been elucidated through comprehensive analyses of the cancer transcriptome. Over the past 20 years, gene expression signatures, discovered with both microarray and RNA-Seq have reached clinical and commercial application through the development of tests such as Mammaprint®, OncotypeDX®, and FoundationOne® CDx, all which focus on chemotherapy sensitivity, prediction of cancer recurrence, and tumour mutational level. The Gene Expression Barcode (GExB) algorithm was developed to allow for easy interpretation and integration of microarray data through data normalization with frozen RMA (fRMA) preprocessing and conversion of relative gene expression to a sequence of 1's and 0's. Unfortunately, the algorithm has not yet been developed for RNA-Seq data. However, implementation of the GExB with feature-selection would contribute to a machine-learning based robust breast cancer and subtype classifier. METHODOLOGY: For microarray data, we applied the GExB algorithm to generate barcodes for normal breast and breast tumour samples. A two-class classifier for malignancy was developed through feature-selection on barcoded samples by selecting for genes with 85% stable absence or presence within a tissue type, and differentially stable between tissues. A multi-class feature-selection method was employed to identify genes with variable expression in one subtype, but 80% stable absence or presence in all other subtypes, i.e. 80% in n-1 subtypes. For RNA-Seq data, a barcoding method needed to be developed which could mimic the GExB algorithm for microarray data. A z-score-to-barcode method was implemented and differential gene expression analysis with selection of the top 100 genes as informative features for classification purposes. The accuracy and discriminatory capability of both microarray-based gene signatures and the RNA-Seq-based gene signatures was assessed through unsupervised and supervised machine-learning algorithms, i.e., K-means and Hierarchical clustering, as well as binary and multi-class Support Vector Machine (SVM) implementations. RESULTS: The GExB-FS method for microarray data yielded an 85-probe and 346-probe informative set for two-class and multi-class classifiers, respectively. The two-class classifier predicted samples as either normal or malignant with 100% accuracy and the multi-class classifier predicted molecular subtype with 96.5% accuracy with SVM. Combining RNA-Seq DE analysis for feature-selection with the z-score-to-barcode method, resulted in a two-class classifier for malignancy, and a multi-class classifier for normal-from-healthy, normal-adjacent-tumour (from cancer patients), and breast tumour samples with 100% accuracy. Most notably, a normal-adjacent-tumour gene expression signature emerged, which differentiated it from normal breast tissues in healthy individuals. CONCLUSION: A potentially novel method for microarray and RNA-Seq data transformation, feature selection and classifier development was established. The universal application of the microarray signatures and validity of the z-score-to-barcode method was proven with 95% accurate classification of RNA-Seq barcoded samples with a microarray discovered gene expression signature. The results from this comprehensive study into the discovery of robust gene expression signatures holds immense potential for further R&F towards implementation at the clinical endpoint, and translation to simpler and cost-effective laboratory methods such as qtPCR-based tests

Locus of control and online learning.

The integration of online learning in university courses is considered to be both inevitable and necessary. Thus there is an increasing need to raise awareness among educators and course designers about the critical issues impacting on online learning. The aim of this study, therefore, was to assess the differences between two groups of first-year Business Sciences learners (online and conventional learners) in terms of biographic and demographic characteristics and locus of control. The study population consisted of 586 first-year learners of whom 185 completed the Locus of Control Inventory (LCI). The results show that the two groups of learners do not differ statistically significantly from each other with respect to locus of control. The findings and their implications are also discussed

Revitalisation of a historic building through performance evaluative research into workplace environmental conditions

Following the centennial celebrations of the University of the Free State in 2004, the need was identified to initiate a program according to which the image and future development of the university as a whole could be assessed. This included optimising the use of existing physical resources and prioritising on proposed new development. As part of this program, the historic Main Building was identified for revitalisation through an adaptive reuse program into office space for the executive management of the university. Completed in July 2005, the refurbished facility now provides an updated approach to the concept of workplace by providing a mix of private and open plan offices. In an attempt to introduce the concept of performance evaluative research in workplace environments to students of architecture at the University of the Free State, the revitalised Main Building workplace environment was regarded as a suitable example. Performance evaluative research in workplace environments is a form of evidence-based research which aims to assess the functionality and serviceability levels of a specific facility on the basis of its ability to support and facilitate diverse work styles, as well as the user-needs and -requirements associated with it. The evaluation process was conducted according to the principles of an Investigative-level Post-Occupancy Evaluation (POE) study, and aimed to assess the impact of stimuli from the workplace environment on the ability of users to optimally perform and accomplish work-related tasks. The methodology according to which information for this POE study was collected consisted of various surveys. This included an activity survey, an activity duration survey, a communication survey, and a movement survey. The POE study lasted one working day, with the various mapping surveys undertaken simultaneously in 15-minute periods with 5-minute rest and preparation intervals between them. Data collected in this manner thus provides a comprehensive view of all aspects pertaining to work performance and accomplishment in the specific office environment. The survey also included questionnaires distributed to all occupants of the workplace with a return rate of 86%. Conclusions drawn from results of this study have indicated the response of users to the new workplace environment in terms of their perception of the impact of the overall work environment on their ability to concentrate and perform work-related tasks. In this regard problems related to limited or inconvenient access to office resources and problems related to poor thermal comfort (specifically with regards to the penetration of direct sunlight and lack of control over air-conditioning) occurred with a similar frequency (N = 48; f = 0.15). Results of this study will:• serve as feedback for the continued alignment of the University’s physical resources with its dynamic resource management strategy — ; and• contribute to a database on work performance characteristics in South Africa

Die drenkeling in die werk van D.J. Opperman, met spesiale verwysing na Joernaal van Jorik

Bibliography: pages 154-155.In discussing the drowning figure in the work of DJ Opperman, it is assumed that the themes of decline and resurgence are central to the work of the author. With respect to these dual motifs the following became apparent: (a) that the decline inevitably leads to new life, and of necessity, must precede it. (b) This universal paradox of decline and resurgance is characterised by an endless repetition in time. (c) Man as an individual is inextricably caught between the dichotomous forces of birth and death. The individual is recognisable throughout Opperman's work. Through man's intimate connection with water as a medium of birth and death, the individual or "enkeling" is also seen as the drowned figure or "drenkelingfiguur". Reference to this drowned figure carries the joint connotations of drowning or submergence and decline. The condition of decline indicates the disappearance of the drowning figure below the water-line or surface. Such alienation can also be brought about by means of other elements which can become the metaphorical equivalent of water as the medium of submergence

Filament identification and dominance of Eikelboom Type 0092 in activated sludge from wastewater treatment facilities in Cape Town, South Africa

Routine characterisation of activated sludge and identification of the filamentous population by microscopic and/or other non-culture dependent techniques can assist in diagnosing the aetiology of poor performance of wastewater treatment works (WWTWs). In South Africa, most facilities rely solely on physicochemical indicators, treating reactors as ‘black-boxes’, with the result that process adjustments are often delayed, to the detriment of the environment. This study was performed in order to gain insight into the filamentous population found in activated sludge in Cape Town WWTWs, to compare these with other global and local literature findings, and to build capacity in this science. Physicochemical and plant performance parameters, in terms of nutrient removal and settling, were obtained from routine operational data and assessed in conjunction with the microscopic analyses of activated sludge samples taken over a 6-month period. Hypotheses on the links between filament types and/or plant  configurations and/or operational parameters were formulated using  existing literature. In order of prevalence, the five most common dominant filament species in 96 activated sludge samples were: Eikelboom Type  0092, Eikelboom Type 1851, nocardioforms, Microthrix parvicella and  Eikelboom Type 021N. In order to compile a statistically significant  database, it is recommended that an extensive nationwide study is  conducted to link filament types with plant configurations, operational parameters and geographical locations.Keywords: activated sludge, bulking, identification, filament, Type 009

Share price reaction to Financial Mail’s “Top Companies” announcements

Responsible Investment considers environmental, social and corporate governance criteria. These criteria, as an investment strategy, aim to have a positive impact on society as well as maximize financial returns. The concept of Responsible Investment is becoming more prominent and important to investors, both internationally and locally, with evidence from the negative reaction of share prices to recent events such as the BP oil spill. The Johannesburg Stock Exchange, in collaboration with FTSE4Good, has developed Responsible Investment criteria. The aim of the criteria is to ensure global alignment, with regards to environmental criteria, and also ensure local relevance, with criteria that deals with issues such as black economic empowerment, skills development and HIV/Aids. This research examines the share price behaviour of companies that are recommended by analysts as ‘Top Companies’ on the basis of their compliance to set Responsible Investment criteria, with specific reference to the annual ‘Top 20 Companies’ as recommended by the Financial Mail magazine. Using event study methodology, the short- and long-term behaviour is studied for the 140 companies mentioned in the list from 2003 up until 2009. Positive, significant abnormal returns of around 2% are observed in the first 10 days following the announcement for the companies mentioned in the list for the first time. No positive abnormal returns are however observed for longer-term holding periods of up to 200 days following the announcement. The result suggests that the Financial Mail analysts’ annual recommendation is of value only to low transaction cost, short-term traders. Longer-term investors, who buy the recommended shares, generally receive returns similar to the market rate of return. CopyrightDissertation (MBA)--University of Pretoria, 2010.Gordon Institute of Business Science (GIBS)unrestricte

Let the Ancestors Speak: an archaeological excavation and re-evaluation of events prior and pertaining to the 1854 siege of Mugombane, Limpopo Province, South Africa

Student Number : 8534741 - PhD thesis - School of Geography, Archaeology and Environmental Studies - Faculty of ScienceDuring the 19th century the present Makapan Valley World Heritage site was a place of repeated refuge from the conflicts arising from shifting authority, acquisition and loss of power, and competition over the control of resources in the northern regions of Republic of South Africa. During 1854 growing resistance amongst the northern AmaNdebele against the frontier of colonial expansion erupted in the murder of a number of trekboers who were encroaching on their territory. Historic Cave, one of the caves in the Valley, became written into the Afrikaner Nationalist narratives of the 20th century as the place where the Boers avenged the treacherous murder of their fellow trekkers, by suppressing the savage forces of chief Mugombane and his Kekana chiefdom. The events surrounding the siege and the scale of the massacre became blurred in the playing out of these political agendas, while the Kekana oral histories remained silent on the matter. The excavation of Historic Cave, prompted by the contradictions in the historical narrative and the silence in the oral record, provided a means to detect the boundary between what happened and what was said to have happened. This thesis presents the results of a survey of the Valley, the excavation of Historic Cave and the analysis of the remains of the siege of 1854. The archaeological survey and excavation indicates that a number of scurmishes took place in the valley, but that Historic Cave was occupied only on one occasion for any length of time, during the siege of 1854. The lime enriched deposits and dry conditions within the cave have allowed for the exceptional preservation of plant, animal and human remains. This enduring evidence chronicled the activities and steps taken by the Kekana to survive. The spatial layout of the cave together with cultural remains echo the structure and hierarchy of the society trapped within it, which like many African societies of this time comprised a heterogeneous ‘royal’ core and other ‘foreign’ subordinate groups. Evidence for divination reflects the final attempts to divine the cause of misfortune and protect the group against maleficent forces. However, it is argued that the social and religious forces that operated to keep the chiefdom together may have begun to loosen under the pressure of the siege, especially as the polluting forces of death became stronger and the group began to succumb. The remains of young and old people, and the desiccated bodies of a child and a young woman speak of untold suffering and provide a glimpse of the horror within the cave. From this it is reasoned that following the devastation of the siege the core of the chiefdom was challenged; the chief was ‘dealt with’ and the political power base shifted. The real reason for the ‘silence’ then lies at the point of rupture, at the stage when the surviving statesmen contrived a suitable account of ensuing events to give the new chief legitimacy and the lineage continuity

Orem's theory applied in the community health practice

Summaries in Afrikaans and EnglishDie doelwitte van hierdie studie is om een van die teoriee wat by opleidingsinstellings gedoseer word, in die gemeenskapgesondheidpraktyk op tienergesinne toe te pas en om 'n strategie te ontwikkel wat sodanige toepassing vir verpleegkundiges aanvaarbaar maak. Orem se selfsorgtekortteorie is gevolglik op gevallestudies by 'n Gemeenskapgesondheidskliniek van 'n stadsgesondheidsafdeling toegepas. Persoonlike onderhoudvoering is as navorsingstegniek gebruik om probleme te identif iseer, behoeftes te bepaal en selfsorgtekorte aan te spreek. Data is gemeet aan die hand van UNICEF se GOBI FFFF en die PKK se vyf konsepte, die verpleegproses en OREM SE KONSTRUKTE. Evaluasie toon dat Orem se selfsorgteorie 'n instrument bied om selfsorgvermoens te evalueer, die verpleegkundige te rig om tekorte te identifiseer en verpleegsorg te beplan sodat interdissiplinere konsultasie verminder en professionele verpleegkundiges beter aangewend word, hulpbronne en fasiliteite maksimaal benut word en elke indiwidu as selfsorgagent ontwikkel word.The objective of this study is to apply one of the theories taught at training institutions to teenage families in the community health practice and to develop a strategy to make such application acceptable to nurses. Orem' s self-care deficiency theory was consequently applied to case studies at a community clinic of a Municipal Health Department. Personal interviews were mainly used as research technique to identify problems, determine needs and address self-care deficiencies. Data was evaluated according to UNICEF' s GOBI FFFF, the "PKK" 's five concepts, the nursing process and OREM's Constructs. Evaluation has proved that Orem' s theory offers an instrument to evaluate self-care abilities and guide the nurse in identifying deficiencies and planning nursing care in order to reduce multidisciplinary consultation, which results in professional nurses being employed appropriately, resources being utilised to the maximum and each individual developing into a complete self-care agent.Health StudiesM.A. (Verpleegkunde