Cytotoxic effect of a new endodontic cement and mineral trioxide aggregate on L929 line culture

Introduction: The aim of this study was to compare the cytotoxicity of Mineral Trioxide Aggregate (MTA) and a New Endodontic Cement (NEC) on L929 mouse fibroblasts. Materials and Methods: Different dilutions (Neat, 1/2, 1/10, 1/100) of fresh and set materials placed adjacent flasks of L929 in DMEM medium. Cellular viability was assessed using MTT assay in three time intervals (24, 48, and 72 h after mixing). Differences in mean cell viability values between materials were assessed by using the One-way ANOVA and Bonferoni post-test. Optical microscopic analysis of morphology of the untreated control and the cement-treated cell cultures were carried out in all experimental periods. Results: It was indicated that there was not a significant difference in cytotoxicity among the materials of test and between them and the control group. However, there was a statistically significant difference between different time intervals within each group (P< 0.05) and between different concentration of test materials (P<0.05). In all samples, set materials showed better viability than fresh ones. Conclusion: According to results of this study, NEC and MTA have similar cytotoxic effect on L929 cell culture

The effect of Tranexamic acid on cardiac surgery bleeding

Serious bleeding in cardiac surgery leads to re-exploration, blood transfusion and increases the risks of mortality and morbidity. Using the lysine analogous of antifibrionlytic agents are the preferred strategy to suppress the need for transfusion procedures and blood products. Although tranexamic acid has been very influential in reducing the transfusion requirement after operation, tranexamic acid induced seizures is one of the common side effects of this drug. Due to inhibiting the fibrinolysis, thrombotic events are other possible side effects of using tranexamic acid. There are no certain results regarding decreasing the mortality rate by using the drug but it is identified that tranexamic acid does not increase the mortality. In this article, we aimed to review the literature on using tranexamic acid in cardiac surgeries

The Effect of Hydroalcoholic Extract of Tamarix dioica Flower on Thioacetamide - Induced Histomorphological Changes in Rats

Background and Objectives: The present experimental study was aimed to investigate the effect of T. dioica flower hydroalcoholic extract on thioacetamide induced histopathological changes.   Methods: Thirty adult male Wistar rats, were randomly divided into three groups of control (normal saline); negative control (thioacetamide 50 mg/kg) and group that received thioacetamide and T. dioica flower extract (200 mg/kg). The third group received the T. dioica flowers extract two weeks before the injection of thioacetamide and two weeks after the injection of thioacetamide. The healthy control group and the negative control group received normal saline. At the end of the experiment, blood samples, were obtained from the heart to determine lever enzymes aspartate aminotransferase (AST), alanine aminotransaminase (ALT), blood urea nitrogen (BUN), and creatinine. After euthanasia, tissue samples, were examined using a light microscope after staining with haematoxylin-eosin. Data were analyzed by one-way ANOVA statistical test. Statistical significance was considered as p < 0.05.   Results: The levels of serum liver enzyme, were significantly increased after the administration of thioacetamide. The administration of T. dioica extract significantly decreased serum ALT and AST levels compared to the negative control group (p < 0.05). Moreover, treatment with T. dioica extract significantly reduced serum BUN and creatinine levels compared to the negative control group (p < 0.05). In the histomorphometric investigation of liver, the fatty change and necrosis was lower in the T. dioica – treated group compared to the negative control group (p < 0.05). Renal histopathological examination also revealed lower kidney damage compared to the group treated with thioacetamide.   Conclusion: hydro-alcoholic extract of T. dioica flowers can reduce thioacetamide induced liver and kidney injury