Plasma FGF23, urinary excretion of P and renal NaPiIIa.

<p><b>(A)</b> Circulating concentrations of FGF23 in rats (n = 8 per group) fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP). (B) Urinary excretion of P (mg/day) in rats (n = 8 per group) fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP). (C) Expression of NaPiIIa in the kidneys of rats (n = 8 per group) fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP). The original gel from which blots have been extracted is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198481#pone.0198481.s001" target="_blank">S1 Fig</a>. ^ap<0.05 vs NF-NP, ^bp<0.05 vs HF-NP.</p

Body weight.

<p>Increase in body weight after the 7 months that lasted the experiments in rats (n = 8 per group) fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP). ^ap<0.05 vs NF-NP.</p

Blood biochemistry.

<p>Plasma concentrations of parameters related to energy metabolism, mineral metabolism, renal function, and inflammation in rats fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP).</p

Renal histopathology.

<p>Glomerular retraction and sclerosis (Glomerular lesions); tubular atrophy, hyperplasia and thickening of basement membrane (Tubular lesions); and interstitial edema and infiltrate (Interstitial lesions) in rats fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP).</p

Diet vs urine & plasma P and FGF23.

<p>Correlation between P ingested and (A) P urine (r² = 0.663, p<0.001), (B) P plasma (r² = 0.004, p = 0.806), and (C) FGF23 plasma (r² = 0.130, p = 0.187) in rats fed high fat diets with normal and low phosphorus content.</p

Renal klotho.

<p>Klotho mRNA (A) and protein (B) in the kidneys of rats (n = 8 per group) fed diets with normal fat and normal phosphorus (NF-NP), high fat and normal phosphorus (HF-NP) and high fat and low phosphorus (HF-LP). The original gel from which blots have been extracted is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0198481#pone.0198481.s002" target="_blank">S2 Fig</a>. ^ap<0.05 vs NF-NP.</p

Haematological and biochemical parameters of 28 dogs with leishmaniasis and CKD before (at day 0) and after treatment (at day 28).

<p>Haematological and biochemical parameters of 28 dogs with leishmaniasis and CKD before (at day 0) and after treatment (at day 28).</p

Individual renal function biomarkers of the dogs under study before (at day 0) and after (at day 28) treatment with marbofloxacin.

<p>Individual renal function biomarkers of the dogs under study before (at day 0) and after (at day 28) treatment with marbofloxacin.</p

Principal components analysis plot.

<p><b>Interrelation between the main parameters studied at day 0 (A) and at day 28 (B)</b>. Alb: albumin; AbTiter: antibody titer; Crea: creatinine; CScore: clinical score; DBP: diastolic blood pressure; Glob: globulins; P: phosphate; ParLoad: parasitic load; PCV: packed cell volume; SBP: systolic blood pressure; UCystatin: urine cystatin C; UFerritin: urine ferritin; UPC: urine protein to creatinine ratio.</p

Concentrations of plasma creatinine (A), plasma urea (B) and urine protein to creatinine (UPC) ratio (C) on days 0, 7, 14, 21 and 28 of treatment with marbofloxacin in the dogs under study.

<p>Values are expressed as the mean±standard deviation.</p