% compliance with 15 MIQE parameters by all 461 publications between 2009 and 2013.

<p>The hatched grey line indicates 50% compliance.</p

Data analysis method.

<p>Eleven steps needed for the analysis of 461 scientific papers related to RT-qPCR.</p

Quality and compliance with MIQE guideline analysed criteria.

<p>Quality and compliance with MIQE guideline analysed criteria.</p

Overall compliance with MIQE guidelines of category A papers (journals with IF<5, n = 402) compared to category B papers (journals with IF>5, n = 59).

<p>Neither dataset passed the D'Agostino & Pearson and Shapiro&Wilks normality tests, hence the nonparametric Mann-Whitney test was used for data analysis).</p

Comparison of online supplement utilization and MIQE compliance between publications with IF<5 (grey) and those ≥5 (black).

<p>Both datasets passed the D'Agostino & Pearson and Shapiro&Wilks normality tests, hence the parametric paired t-test was used for data analysis). The hatched grey line indicates 50% compliance.</p

Docking Studies of Pakistani HCV NS3 Helicase: A Possible Antiviral Drug Target

<div><p>The nonstructural protein 3 (NS3) of hepatitis C virus (HCV) helicase is believed to be essential for viral replication and has become an attractive target for the development of antiviral drugs. The study of helicase is useful for elucidating its involvement in positive sense single-stranded RNA virus replication and to serve as templates for the design of novel antiviral drugs. In recent years, several models have been proposed on the conformational change leading to protein movement and RNA unwinding. Some compounds have been recently reported to inhibit the helicase and these include small molecules, RNA aptamers and antibodies. The current study is designed to help gain insights for the consideration of potential inhibitors for Pakistani HCV NS3 helicase protein. We have cloned, expressed and purified HCV NS3 helicase from Pakistani HCV serum samples and determined its 3D structure and employed it further in computational docking analysis to identify inhibitors against HCV genotype 3a (GT3a),including six antiviral key molecules such as quercetin, beta-carotene, resveratrol, catechins, lycopene and lutein. The conformation obtained after docking showed good hydrogen bond (HBond) interactions with best docking energy for quercetin and catechins followed by resveratrol and lutein. These anti-helicase key molecules will offer an alternative attraction to target the viral helicase, due to the current limitation with the interferon resistance treatment and presences of high rate of resistance in anti-protease inhibitor classes.</p></div

Targeted residues in interaction and their docking score.

<p>Targeted residues in interaction and their docking score.</p

Comparison of active hit compounds docked against modeled HCV NS3 GT3a.

<p>Comparison of active hit compounds docked against modeled HCV NS3 GT3a.</p

20% SDS Gel showing purified protein by ion exchange chromatography.

<p>Lane 2–7, Different fractions with non-specific proteins; Lane 8–10, NS3 helicase GT3a purified and separated fractions after FPLC; Lane 11 & 14, NS3 helicase with low concentration and non-specific proteins; Lane 15, Control Con1 GT1b helicase.</p

Comparing the docking interaction with ten hit compounds with HCV GT3a NS3 helicase such as 1) SID 17513061 2) SID 3716320 3) SID 17513201 4) SID 17401675 5) SID 49666882 6) SID 24818609 7) SID 24827353 8) SID 49732586 9) SID 4257236 10) SID 49817864 obtained from PubChem Bioassay.

<p>Comparing the docking interaction with ten hit compounds with HCV GT3a NS3 helicase such as 1) SID 17513061 2) SID 3716320 3) SID 17513201 4) SID 17401675 5) SID 49666882 6) SID 24818609 7) SID 24827353 8) SID 49732586 9) SID 4257236 10) SID 49817864 obtained from PubChem Bioassay.</p