7 research outputs found
Investigation into background levels of small organic samples at the NERC Radiocarbon Laboratory
Recent progress in preparation/combustion of submilligram organic samples at our laboratories is presented. Routine methods had to be modified/refined to achieve acceptable and consistent procedural blanks for organic samples smaller than 1000 g C. A description of the process leading to a modified combustion method for smaller organic samples is given in detail. In addition to analyzing different background materials, the influence of different chemical reagents on the overall radiocarbon background level was investigated, such as carbon contamination arising from copper oxide of different purities and from different suppliers. Using the modified combustion method, small amounts of background materials and known-age standard IAEA-C5 were individually combusted to CO2. Below 1000 g C, organic background levels follow an inverse mass dependency when combusted with the modified method, increasing from 0.13 0.05 pMC up to 1.20 0.04 pMC for 80 g C. Results for a given carbon mass were lower for combustion of etched Iceland spar calcite mineral, indicating that part of the observed background of bituminous coal was probably introduced by handling the material in atmosphere prior to combustion. Using the modified combustion method, the background-corrected activity of IAEA-C5 agreed to within 2 s of the consensus value of 23.05 pMC down to a sample mass of 55 g C
Progress in AMS target production in sub-milligram samples at the NERC Radiocarbon Laboratory
. Recent progress in graphite target production for sub-milligram environmental samples in our facility is presented.
We describe an optimized hydrolysis procedure now routinely used for the preparation of CO2 from inorganic samples,
a new high-vacuum line dedicated to small sample processing (combining sample distillation and graphitization units),
as well as a modified graphitization procedure. Although measurements of graphite targets as small as 35 µg C have been
achieved, system background and measurement uncertainties increase significantly below 150 µg C. As target lifetime can
become critically short for targets <150 µg C, the facility currently only processes inorganic samples down to 150 µg C. All
radiocarbon measurements are made at the Scottish Universities Environmental Research Centre (SUERC) accelerator mass
spectrometry (AMS) facility. Sample processing and analysis are labor-intensive, taking approximately 3 times longer than
samples ≥500 µg C. The technical details of the new system, graphitization yield, fractionation introduced during the process,
and the system blank are discussed in detail
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T-Cell Infiltration and Adaptive Treg Resistance in Response to Androgen Deprivation With or Without Vaccination in Localized Prostate Cancer
Purpose: Previous studies suggest that androgen deprivation therapy (ADT) promotes antitumor immunity in prostate cancer. Whether a vaccine-based approach can augment this effect remains unknown.
Experimental Design: Therefore, we conducted a neoadjuvant, randomized study to quantify the immunologic effects of a granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting allogeneic cellular vaccine in combination with low-dose cyclophosphamide (Cy/GVAX) followed by degarelix versus degarelix alone in patients with high-risk localized prostate adenocarcinoma who were planned for radical prostatectomy.
Results: Both Cy/GVAX plus degarelix and degarelix alone led to significant increases in intratumoral CD8+ T cell infiltration and PD-L1 expression as compared to a cohort of untreated, matched controls. However, the CD8+ T cell infiltrate was accompanied by a proportional increase in regulatory T cells (Treg), suggesting that adaptive Treg resistance may dampen the immunogenicity of ADT. Although Cy/GVAX followed by degarelix was associated with a modest improvement in time-to-PSA progression and time-to-next treatment as well as an increase in PD-L1, there was no difference in the CD8 T-cell infiltrate as compared to degarelix alone. Gene expression profiling demonstrated that CHIT1, a macrophage marker, was differentially upregulated with Cy/GVAX plus degarelix compared to degarelix alone.
Conclusions: Our results highlight that ADT with or without Cy/GVAX induces a complex immune response within the prostate tumor microenvironment. These data have important implications for combining ADT with immunotherapy. In particular, our finding that ADT increases both CD8+ T cells and Tregs, supports the development of regimens combining ADT with Treg-depleting agents in the treatment of prostate cancer
Investigation on the chemical structure of non extractable residues of the fungicide cyprodinil in spring wheat using 13C-C1-phenyl-cyprodinil on 13C-depleted plants û an alternative approach to investigate non-extractable residues
Birnessite-Induced Binding of Phenolic Monomers to Soil Humic Substances and Nature of the Bound Residues
Functions, duplication and evolution of the CYP98 cytochrome P450 family in wheat and Arabidopsis
Stimulation of Tetrabromobisphenol A Binding to Soil Humic Substances by Birnessite and the Chemical Structure of the Bound Residues
Studies
have shown the main fate of the flame retardant tetrabromobisphenol
A (TBBPA) in soils is the formation of bound residues, and mechanisms
on it are less-understood. This study investigated the effect of birnessite
(δ-MnO<sub>2</sub>), a naturally occurring oxidant in soils,
on the formation of bound residues. <sup>14</sup>C-labeled TBBPA was
used to investigate the pH dependency of TBBPA bound-residue formation
to two soil humic acids (HAs), Elliott soil HA and Steinkreuz soil
HA, in the presence of δ-MnO<sub>2</sub>. The binding of TBBPA
and its transformation products to both HAs was markedly increased
(3- to 17-fold) at all pH values in the presence of δ-MnO<sub>2</sub>. More bound residues were formed with the more aromatic Elliott
soil HA than with Steinkreuz soil HA. Gel-permeation chromatography
revealed a uniform distribution of the bound residues within Steinkreuz
soil HA and a nonuniform distribution within Elliott soil HA. <sup>13</sup>C NMR spectroscopy of <sup>13</sup>C-TBBPA residues bound
to <sup>13</sup>C-depleted HA suggested that in the presence of δ-MnO<sub>2</sub>, binding occurred via ester and ether and other types of
covalent bonds besides HA sequestration. The insights gained in this
study contribute to an understanding of the formation of TBBPA bound
residues facilitated by δ-MnO<sub>2</sub>