Impaired IL-23-dependent induction of IFN-gamma underlies mycobacterial disease in patients with inherited TYK2 deficiency

Human cells homozygous for rare loss-of-expression (LOE) TYK2 alleles have impaired, but not abolished, cellular responses to IFN-alpha/beta (underlying viral diseases in the patients) and to IL-12 and IL-23 (underlying mycobacterial diseases). Cells homozygous for the common P1104A TYK2 allele have selectively impaired responses to IL-23 (underlying isolated mycobacterial disease). We report three new forms of TYK2 deficiency in six patients from five families homozygous for rare TYK2 alleles (R864C, G996R, G634E, or G1010D) or compound heterozygous for P1104A and a rare allele (A928V). All these missense alleles encode detectable proteins. The R864C and G1010D alleles are hypomorphic and loss-of-function (LOF), respectively, across signaling pathways. By contrast, hypomorphic G996R, G634E, and A928V mutations selectively impair responses to IL-23, like P1104A. Impairment of the IL-23-dependent induction of IFN-gamma is the only mechanism of mycobacterial disease common to patients with complete TYK2 deficiency with or without TYK2 expression, partial TYK2 deficiency across signaling pathways, or rare or common partial TYK2 deficiency specific for IL-23 signaling.ANRS Nord-Sud ; CIBSS ; CODI ; Comité para el Desarrollo de la Investigación ; Fulbright Future Scholarshi

Utilization of ASTER data in lithological and lineament mapping of the southern flank of the Central High Atlas in Morocco

Geological mapping undoubtedly plays an important role in several studies and remote sensing data are of great significance in geological mapping, particularly in poorly mapped areas situated in inaccessible regions. In the present study, Principal Component Analysis (PCA), Band Rationing (BR) and Minimum Noise Fraction (MNF) algorithms are applied to map lithological units and extract lineaments in the Amezri-Amassine area, by using multispectral ASTER image and global digital elevation model (GDEM) data for the first time. Following preprocessing of ASTER images, advanced image algorithms such as PCA, BR and MNF analyses are applied to the 9ASTER bands. Validation of the resultant maps has relied on matching lithological boundaries and faults in the study area and on the basis of pre-existing geological maps. In addition to the PCA image, a new band-ratio image, 4/6–5/8–4/5, as adopted in the present work, provides high accuracy in discriminating lithological units. The MNF transformation reveals improvement over previous enhancement techniques, in detailing most rock units in the area. Hence, results derived from the enhancement techniques show a good correlation with the existing litho-structural map of the study area. In addition, the present results have allowed to update this map by identifying new lithological units and structural lineaments. Consequently, the methodology followed here has provided satisfactory results and has demonstrated the high potential of multispectral ASTER data for improving lithological discrimination and lineament extraction

Lithological mapping and automatic lineament extraction using Aster and Gdem data in the Imini-Ounilla-Asfalou district, South High Atlas of Marrakech, Morocco

Lithological and lineament mapping using remote sensing is a fundamental step in various geological studies, as it forms the basis for the interpretation and validation of the results obtained. There were two objectives for this study, applied in the Imini-Ounilla-Asfalou district, South High Atlas of Marrakech region: first, lithological mapping by satellite image processing techniques such as ASTER L1B (hight spectral and spatial resolution), namely Principal Component Analysis (PCA), Minimum Noise Fraction (MNF), as well as the application of three types of supervised classification, namely Spectral Angle Mapper (SAM), Maximum Likelihood (ML) and Minimum Distance (MD), on the visible/near-infrared (VNIR) and short-wave infrared (SWIR) spectral bands of our ASTER image; second, an analysis of the distribution of lineaments by automatic extraction using a Global Digital Elevation Model (GDEM) and the PC1 image derived from the PCA transformation applied to the satellite image. The best results are highlighted by the delineation of new facies in relation to the existing map; after confirmation in the field, all of these facies, which include Eocene, Triassic and Jurassic formations, are represented on the new map. The results of lineaments showed that each of them systematically shows a similarity in terms of concentration and orientation, with four preferential oriented systems: NE-SW, E-W, NNE-SSW and NW-SE. The lineaments mainly follow those of the major fault zones, with high concentrations in the northeast and southwest parts of the study area