Penapisan Virtual Senyawa Turunan Metil Sinamat Pada Enzim Siklooksigenase-2 (Cox-2

Inflammation is the response of living tissue to vascular injury. Non-Steroid Anti-Inflammatory Drugs(NSAIDs) could control pain caused by inflammation. Therapeutic effects of NSAIDs are related to themechanism of the cyclooxygenase-1 (COX-1) enzyme and the cyclooxygenase-2 (COX-2) enzyme in inhibiting theproduction of prostaglandins. In this paper, we report the virtual screening of methyl cinnamate derivativecompounds against the COX-2. All methyl cinnamate derivative compounds were molecular docking simulatedusing the Protein-Ligand ANT System (PLANTS) software with ZINC03814717 as the reference compound. Fromthe results of virtual screening, it was obtained that derivative compounds of methyl cinnamate were predicted asactive COX-2 inhibitors because they have ChemPLP score better than that of the reference compoundsZINC03814717. The compound of acid 3- (4-benzoylphenyl) propionate was predicted as having the best score ofChemPLP

Bioaktivitas Turunan Metil Sinamat Terhadap Pertumbuhan Bakteri Escherichia Coli, Staphylococcus Aureus, Bacillus Subtilis, Pseudomonas Aureugenosa Dan Jamur Candida Albicans

Methyl cinnamic is a compound isolated from Alpinia malaccensis included in the family Zingiberaceae. A. malaccensis in Indonesia known as galangal forest. Some studies inform that ginger has anti-bacterial and pharmacologically galangal act as an antifungal. In this study the bioactivity of the compound methyl cinnamate and methyl cinnamic derivative which results cinnamic methyl esterification compound on the growth of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aureugenosa and fungus Candida albicans. Methyl cinnamic derivative compounds tested are; cinnamic acid, cinnamic ethyl, butyl and 2-butyl cinnamic cinnamic. Anti-microbial test results showed that of the samples tested, cinnamic acid is able to inhibit microbial growth of S. aureus, B. subtilis, E. coli and P. aureugenosa and fungi C.albicans.DOI :http://dx.doi.org/10.15408/jkv.v0i0.317

Standardization of Pegagan Extract, Centella Asiatica as Hepatoprotectiveherbal Medicine

Herbal medicinal products would be affected by the quality of raw materials. In turn, the quality of raw material will also be influenced by various factors such as soil conditions, cultivation, post-harvest processing, and the processing of raw materials into crude drug or extract. Therefore, in order to make good herbal medicines, it is necessary to make standardization of herbal extracts that produced herbal medicines that have the same quality and functions of effectiveness in each process. From preliminary studies that have been done, Centella asiatica is one of the potential plants as a source of hepatoprotective compounds. Test in vivo and in vitro against Centella asiatica extracts have shown very good results. Ethyl acetate extract with 17.5 mg/kg of doses body weight and butanol 228.8 mg/kgof doses body weight has been applied for in vivo test using mice induced by CCl4; theydemonstrated hepatoprotective effects. Ethyl acetate extracts were able to reduce levels of the enzyme alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by 56 % and 44 % respectively while butanol extract can reduce the enzymes AST levels by 3%. Standardizationof Centella asiatica extract performed in this study was the characterization of the extract in the form of non-specific and specific parameters corresponding to the reference of PPOMN (Ministry of health Republic of Indonesia, 2000) such as levels of drying shrinkage, ash content, total plate count microbial contamination, levels of water-soluble compounds, levels of compounds that are soluble in ethanol, phytochemical test, total phenolic content, total flavonoid content and the determination of Pb and Cd weight.The results showed that non-specific parameters for the ethanol extract of Centella asiatica were requirements based on Herbal Pharmacopoeia in 2008 which includes parameters such as determination of shrinkage on drying ≤ 10%, ash content ≤ 16.6% and negative microbial contamination. Specific parameters for the ethanol extract of Centella asiatica have met the requirements of Herbal pharmacopeia in 2008

Study of Total Phenolic, Total Flavonoid, Scopoletin Contents and Antioxidant Activity of Extract of Ripened Noni Juice

Extraction of ripened noni juice has been carried out using ethanol and water. The purpose of this study was to determine the total phenolic, total flavonoids, scopoletin, content and antioxidant activity of the ripened noni juice extract. The activity test was carried out on the ripened noni juice extract without and with the addition of ethanol and water solvent. The phytochemical assays of ethanol extract of the ripened noni juice showed that thetotal phenolic content was 3,94 mg gallic acid equivalent/g extract, the total flavonoids was 0,59 mg quercetin equivalent /g extract, the IC50 antioxidant activity was 24,92 mg/L, scopoletin content was 2,45 mg/gram

The Synthesis of Quinidine Salicylate Ester Compound

Quinidine a compound isolated from quinine plants, one of the species of quinine plants is (Chincona ledgereriana) From PT SIL Lembang. The purpose of this study was obtain quinidine salicylate ester through esterification reaction. In this study, the synthesis of quinidine ester compound by esterification reaction was conducted. Esterification reaction was conducted by using DCC activator and DMAP catalyst with one carboxylic acid namely salicylate acid producing new compound namely quinidine salicylate, Subsequent Quinidine salicylate was obtained in the form of oil with 97% yield. The compound obtained from the synthesis was then identified using Thin Layer Chromatography continue analyzed using with Spectrophotometer, LC-ESI-MS spectroscopy. Results show that the target compound has been successfully synthesized